FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-69-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-69-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178626
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA36082.0198627299497276No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT23901.3379911099168094No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA22121.2383415628184027No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA19471.0899869000033588No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG18161.0166493119702618No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC15310.8570980708295545No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG14740.825187822601413No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA14720.8240681647688467No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC13760.770324588805661No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT13700.7669656153079619No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA11940.6684357260421215No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT11810.6611579501304401No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC11710.6555596609676083No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG11050.6186109524929181No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT10990.6152519789952191No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT10680.5978972825904404No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC10280.575504125939113No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT9320.5217605499759274No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG8600.48145286800353815No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG8260.4624186848499099No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA7730.4327477522869011No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT7660.4288289498729188No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT7460.4176323715472552No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT7400.41427339804955604No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG7370.4125939113007065No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT7290.40811527997044106No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG7150.4002776751424765No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC7020.3929998992307951No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC6940.3885212679005296No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT6930.38796143898424645No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC6860.38404263657026416No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC6610.37004691366318454No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT6550.36668794016548545No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA6310.353252046174689No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC6290.3521323883421227No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA6210.3476537570118572No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC5950.3330982051884944No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG5900.3302990606070785No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA5830.3263802581930962No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG5740.32134179794654755No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA5740.32134179794654755No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT5710.319662311197698No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC5690.3185426533651316No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT5560.3112648774534502No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA5550.3107050485371671No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTA5520.30902556178831747No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT5440.30454693045805203No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC5320.29782898346265385No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA5270.2950298388812379No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT5240.29335035213238836No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA4990.2793546292253088No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT4970.27823497139274234No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT4860.27207685331362735No Hit
AAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGT4850.27151702439734415No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA4690.2625597617368132No Hit
CATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCA4550.25472215690884864No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC4520.2530426701599991No Hit
GTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGC4480.25080335449486635No Hit
GGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTA4460.2496836966623No Hit
GTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGG4380.2452050653320345No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA4310.24128626291805225No Hit
CCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC4260.2384871183366363No Hit
GTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGA4200.23512814483893724No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA4200.23512814483893724No Hit
GTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCT4180.23400848700637086No Hit
ATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTG4160.23288882917380452No Hit
GAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTAT4040.2261708821784063No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA3980.22281190868070716No Hit
CTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTT3950.22113242193185761No Hit
CTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACG3840.2149743038527426No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT3830.2144144749364594No Hit
GGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATA3670.20545721227592847No Hit
GTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTT3630.20321789661079576No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT3630.20321789661079576No Hit
TAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCT3560.19929909419681346No Hit
GCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACC3480.19482046286654797No Hit
GTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAAT3400.1903418315362825No Hit
CTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGT3370.18866234478743296No Hit
ATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAA3360.1881025158711498No Hit
CCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGT3360.1881025158711498No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA3360.1881025158711498No Hit
TTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTG3350.1875426869548666No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC3300.18474354237345067No Hit
CCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAA3240.18138456887575158No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT3190.17858542429433566No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT3100.173546964047787No Hit
GATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACT3090.1729871351315038No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG3060.17130764838265428No Hit
GTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT3050.17074781946637108No Hit
GCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCAT3000.16794867488495516No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA2940.16458970138725607No Hit
GAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAAT2930.16402987247097287No Hit
GTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGA2910.16291021463840652No Hit
ACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT2910.16291021463840652No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG2870.16067089897327377No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC2860.1601110700569906No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTC2850.1595512411407074No Hit
TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTT2830.15843158330814103No Hit
ATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTG2820.15787175439185785No Hit
ATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTAC2760.15451278089415874No Hit
CTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTA2760.15451278089415874No Hit
TCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACAC2720.15227346522902602No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA2700.15115380739645964No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2670.1494743206476101No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT2630.14723500498247735No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC2610.14611534714991098No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA2540.14219654473592871No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG2530.14163671581964551No Hit
AGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACAC2510.14051705798707914No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2500.13995722907079597No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTT2490.1393974001545128No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT2460.13771791340566322No Hit
ATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAG2460.13771791340566322No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2420.1354785977405305No Hit
TATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAAC2410.1349187688242473No Hit
CTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACAT2400.13435893990796413No Hit
ATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTG2400.13435893990796413No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG2390.13379911099168093No Hit
CCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGAAAA2390.13379911099168093No Hit
GTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGA2370.13267945315911459No Hit
AATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAGTT2350.1315597953265482No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC2320.12988030857769864No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG2320.12988030857769864No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA2300.1287606507451323No Hit
AATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTA2290.12820082182884912No Hit
CACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCA2270.12708116399628272No Hit
TTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGA2240.12540167724743317No Hit
GGGTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTG2230.12484184833115No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTT2200.12316236158230044No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2180.12204270374973408No Hit
GGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATAC2180.12204270374973408No Hit
CTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTG2150.12036321700088454No Hit
CTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATAC2130.11924355916831815No Hit
CCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAA2130.11924355916831815No Hit
CTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTA2110.11812390133575179No Hit
GAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAG2040.11420509892176951No Hit
AAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAATGTAAA2010.11252561217291995No Hit
TTACATTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAA2010.11252561217291995No Hit
ACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGT1990.11140595434035358No Hit
CGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCA1990.11140595434035358No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA1980.11084612542407041No Hit
GTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGT1980.11084612542407041No Hit
CTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTAC1920.1074871519263713No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC1910.10692732301008812No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC1880.10524783626123856No Hit
TAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTG1870.10468800734495537No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC1870.10468800734495537No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA1860.1041281784286722No Hit
TTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGT1850.10356834951238901No Hit
CCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATA1850.10356834951238901No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA1840.10300852059610584No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG1830.10244869167982265No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG1800.10076920493097309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGCC100.007086022145.03
GATACAG100.007086022145.01
GTTCCTA351.0913936E-11145.01
CAGAGGG100.007086022145.09
GCAGAGG100.007086022145.08
CGTGTTC100.007086022145.03
GTAATAT100.007086022145.01
TTTCGGG100.007086022145.03
GTATATG301.0979602E-7120.8333362
TCCTAGG554.129106E-1092.272723
CTAGGTA554.129106E-1092.272725
TTCCTAG554.129106E-1092.272722
CCTAGGT554.129106E-1092.272724
TAGGTAG554.129106E-1092.272726
GCCTTCA259.374037E-487.09
TTATATG259.374037E-487.03
ATTATAT259.374037E-487.02
ATAGGCG608.240022E-1084.5833367
TTAATAG608.240022E-1084.5833364
ATATGCA353.429535E-582.857144
Produced by FastQC (version 0.11.5)