FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-68-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-68-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235123
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA31331.3324940563024459No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT24871.057744244501814No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA22530.9582218668526685No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA18900.8038345887046353No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG16740.7119677785669628No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG15720.6685862293352841No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA15590.663057208354776No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT15260.6490220012504094No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC14850.6315843196964993No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC13360.5682132330737529No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT13150.5592817376437014No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC13060.555453953887965No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT12320.5239810652296883No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC11720.49846250685811255No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA11650.49548534171476205No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA11380.484001990447553No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT11280.4797488973856237No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT11130.47336925779272976No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT11080.47124271126176515No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA10740.4567821948512055No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG10550.4487013180335399No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT10090.4291370899486652No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA10020.4261599248053147No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA9580.40744631533282577No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT9350.39766420129038843No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC9060.3853302314107935No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG8680.36916847777546224No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT8660.36831785916307636No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG8440.3589610544268319No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC8170.34747770315962284No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC7990.3398221356481501No Hit
CTTGTAAATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT7790.3313159495242915No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA7720.32833878438094105No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA7660.3257869285437835No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG7630.3245110006252047No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT7600.3232350727066259No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA7580.32238445409424005No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC7430.3160048145013461No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT7390.3143035772765744No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC7350.31260234005180265No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG6820.29006094682357747No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC6740.286658472374034No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA6730.2862331630678411No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA6600.280704142087333No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT6480.27560043041301785No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA6350.2700714094325098No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG6210.2641170791458088No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT6070.2581627488591078No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA6000.2551855837157573No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT5990.25476027440956434No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT5930.2522084185724068No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT5840.24838063481667041No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA5760.244978160367127No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA5750.24455285106093405No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT5690.2420009952237765No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT5530.23519604632468963No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT5500.23392011840611082No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT5420.2305176439565674No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT5310.2258392415884452No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC5270.22413800436367348No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC5110.21733305546458664No Hit
CACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCA5100.2169077461583937No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA4970.2113787251778856No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG4960.2109534158716927No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG4900.20840156003453508No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA4820.2049990855849917No Hit
AAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGT4730.20117130182925533No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT4730.20117130182925533No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT4640.19734351807351896No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT4590.19521697154255432No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT4550.1935157343177826No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA4520.19223980639920382No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA4430.18841202264346746No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG4420.1879867133372745No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA4390.18671078541869576No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG4330.18415892958153818No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC4310.18330831096915232No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT4300.18288300166295937No Hit
GTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTT4220.17948052721341595No Hit
TTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTC4200.1786299086010301No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC4180.17777928998864423No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG4090.1739515062329079No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT4040.17182495970194325No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA3980.16927310386478567No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA3950.16799717594620686No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTA3930.16714655733382103No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA3920.16672124802762808No Hit
ATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTT3910.16629593872143517No Hit
ATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTG3830.16289346427189175No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3770.16034160843473416No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA3650.15523789676041902No Hit
CTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACG3590.15268604092326143No Hit
CATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCA3570.15183542231087557No Hit
GGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCT3560.15141011300468266No Hit
CTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTT3530.15013418508610388No Hit
ATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCC3530.15013418508610388No Hit
CTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGT3490.14843294786133215No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3470.1475823292489463No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA3440.1463064013303675No Hit
ATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAA3430.14588109202417457No Hit
GAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTAT3430.14588109202417457No Hit
CTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTG3390.14417985479940287No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT3380.14375454549320993No Hit
CCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC3370.143329236187017No Hit
GGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTA3370.143329236187017No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC3320.14120268965605234No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG3280.13950145243128065No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG3270.1390761431250877No Hit
AATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCG3270.1390761431250877No Hit
GACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCAC3220.13694959659412306No Hit
GTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGG3180.13524835936935137No Hit
GTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGC3140.13354712214457964No Hit
GTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCT3130.13312181283838673No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC3090.131420575613615No Hit
CTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTA3070.13056995700122914No Hit
ACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTAATAAAC3040.12929402908265036No Hit
ACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTC3040.12929402908265036No Hit
GTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGA3030.12886871977645742No Hit
TTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTG3000.12759279185787864No Hit
AATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTA2960.12589155463310694No Hit
GCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACC2940.12504093602072108No Hit
ACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT2930.12461562671452814No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT2920.12419031740833521No Hit
ACCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCT2920.12419031740833521No Hit
GTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAAT2900.12333969879594936No Hit
GTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATT2880.1224890801835635No Hit
ACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATT2850.12121315226498472No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC2850.12121315226498472No Hit
GTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGA2840.12078784295879179No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTTTCGATTCAGATCT2830.12036253365259886No Hit
GTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCCATCCAACT2830.12036253365259886No Hit
AAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATA2830.12036253365259886No Hit
TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTT2820.11993722434640593No Hit
TAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCT2800.11908660573402005No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2800.11908660573402005No Hit
CAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTT2790.11866129642782715No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2790.11866129642782715No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2750.11696005920305541No Hit
ATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTG2690.11440820336589784No Hit
ATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTAC2680.11398289405970491No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2670.11355758475351199No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA2640.1122816568349332No Hit
CCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGT2630.11185634752874027No Hit
ATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAG2590.11015511030396856No Hit
GATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACT2580.10972980099777563No Hit
ATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTG2580.10972980099777563No Hit
GCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCAT2570.1093044916915827No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA2550.10845387307919685No Hit
CAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTG2540.10802856377300392No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA2530.10760325446681099No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT2530.10760325446681099No Hit
ACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAG2510.10675263585442513No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA2500.10632732654823221No Hit
GAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTT2490.10590201724203928No Hit
GTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCC2450.10420078001726754No Hit
ACCCTGTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAA2440.10377547071107464No Hit
TAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAG2430.10335016140488171No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC2400.1020742334863029No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC2400.1020742334863029No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2390.10164892418011No Hit
GAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAAT2390.10164892418011No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT2390.10164892418011No Hit
GTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTT2370.10079830556772414No Hit
ACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTT2370.10079830556772414No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA151.2288181E-4145.05
CCTAGGT151.2288181E-4145.04
ACCTAGC151.2288181E-4145.07
CTTGTAA1200.0108.751
CAGGTAT203.86234E-4108.754
GCTGACA203.86234E-4108.758
TCCTAGG203.86234E-4108.753
CTAGGTA203.86234E-4108.755
CTAGCCG203.86234E-4108.759
GGTATAT501.9463187E-10101.51
ATAGGCG607.2759576E-1296.6666645
CAAATGG301.596903E-596.6666643
TGCTGAC259.377755E-487.07
GTTCCTT502.2610038E-887.06
TTCCTAG259.377755E-487.02
GGCGTGT608.258212E-1084.5833368
GCGTGTG608.258212E-1084.5833369
GTAAATC1600.081.56254
CCTTAGG554.3797627E-879.090919
TGCTTAA554.3797627E-879.090911
Produced by FastQC (version 0.11.5)