FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-67-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-67-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG17481.7048502404150938No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC16441.6034175029990931No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG14901.4532190264407838No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG10150.989944504588856No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT8840.8621782680360086No Hit
GATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCA5330.5198427792570053No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC5110.4983858540343896No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT4830.47107704011469703No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC4770.46522515141762005No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG4670.4554720035891584No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA4650.45352137402346604No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG4360.4252372453209273No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA4050.3950024870526963No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC3980.3881752835727731No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA3870.3774468209614653No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTG3730.36379241400161905No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT3640.3550145809560036No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA3570.3481873774760804No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA3360.32770576703631094No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG3120.3042982122480031No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG2780.27113750963123345No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2760.26918688006554115No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2660.25943373223707955No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC2650.25845841745423337No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG2540.24772995484292556No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2530.2467546400600794No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA2520.24577932527723323No Hit
GTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTGCACTTACACCGCA2500.24382869571154092No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG2490.24285338092869474No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG2470.2409027513630024No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC2400.23407554788307927No Hit
CCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTT2370.23114960353454078No Hit
GTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGA2270.22139645570607913No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2150.20969267831192515No Hit
GTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTAC2150.20969267831192515No Hit
GTACAGACTGTGTTTTTAAGTGTAAAACCCACAGGGTCATTAGCACAAGT2130.20774204874623284No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT2060.20091484526630973No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2040.1989642157006174No Hit
GCCATAACCTTTCCACATACCGCAGACGGTACAGACTGTGTTTTTAAGTG2020.19701358613492506No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA2000.19506295656923273No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA1980.1931123270035404No Hit
GTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAAT1970.1921370122206942No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT1970.1921370122206942No Hit
GGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGC1920.1872604383064634No Hit
GTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAAC1910.18628512352361726No Hit
GTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAAC1860.18140854960938643No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA1830.17848260526084794No Hit
GTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGCAACAGGACTAAGC1770.17263071656377096No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA1770.17263071656377096No Hit
GTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTT1770.17263071656377096No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA1740.16970477221523247No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA1730.1687294574323863No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA1690.16482819830100165No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT1690.16482819830100165No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA1690.16482819830100165No Hit
CCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTAC1680.16385288351815547No Hit
ATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTATGACA1670.1628775687353093No Hit
ACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGAC1670.1628775687353093No Hit
GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCA1660.16190225395246316No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT1640.15995162438677085No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT1630.15897630960392467No Hit
GATATATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATG1630.15897630960392467No Hit
CAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAA1600.15605036525538618No Hit
GTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTA1590.15507505047254003No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC1590.15507505047254003No Hit
TATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTA1580.15409973568969385No Hit
CAACAGGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTT1580.15409973568969385No Hit
GGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTG1570.1531244209068477No Hit
AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT1570.1531244209068477No Hit
GGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCAC1570.1531244209068477No Hit
GTTCTGTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCAT1560.15214910612400154No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC1560.15214910612400154No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC1530.14922316177546302No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA1520.14824784699261687No Hit
TTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC1520.14824784699261687No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC1510.1472725322097707No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC1500.14629721742692453No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC1480.14434658786123222No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT1470.14337127307838604No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT1470.14337127307838604No Hit
CAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTA1470.14337127307838604No Hit
GTGCCGCACGGTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAA1460.1423959582955399No Hit
CCTACAAGGTGGTTCCAGTTCTGTATAGATAGTACCAGTTCCATCACTCT1450.1414206435126937No Hit
GTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGC1440.14044532872984755No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT1440.14044532872984755No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA1420.13849469916415522No Hit
AGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGG1410.13751938438130906No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATA1400.1365440695984629No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG1390.13556875481561673No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG1390.13556875481561673No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC1390.13556875481561673No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC1390.13556875481561673No Hit
GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA1370.1336181252499244No Hit
GAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTG1350.13166749568423208No Hit
GGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGT1350.13166749568423208No Hit
CGGTACAGACTGTGTTTTTAAGTGTAAAACCCACAGGGTCATTAGCACAA1340.13069218090138593No Hit
CATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTC1330.12971686611853978No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCT1320.1287415513356936No Hit
CATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTGCACTTACA1320.1287415513356936No Hit
GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAAT1320.1287415513356936No Hit
CACTTACACCGCAAACCCGTTTAAAAACGATTGTGCATCAGCTGACTGAA1310.12776623655284744No Hit
GTTACAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTC1310.12776623655284744No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC1300.12679092177000129No Hit
TCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACAT1300.12679092177000129No Hit
GTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGG1300.12679092177000129No Hit
GTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATAGATAGTACCAG1300.12679092177000129No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC1290.1258156069871551No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA1290.1258156069871551No Hit
ATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAA1280.12484029220430895No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA1280.12484029220430895No Hit
GAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGAT1270.12386497742146278No Hit
GTTATTGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATT1270.12386497742146278No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC1260.12288966263861661No Hit
GTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCCCCCACTAG1260.12288966263861661No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1260.12288966263861661No Hit
GTTTTCAAGACAACTTCCTCTGTTAACACTTCTGTGGGAAGTGTTTCTCC1260.12288966263861661No Hit
CTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATAG1250.12191434785577046No Hit
ATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGT1250.12191434785577046No Hit
ATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCA1240.12093903307292429No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA1240.12093903307292429No Hit
GATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAAATCCT1240.12093903307292429No Hit
TCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA1220.11898840350723196No Hit
CAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCG1220.11898840350723196No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC1210.11801308872438579No Hit
CTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGA1210.11801308872438579No Hit
TCTTAACACAATTAGTGATTGGTTGTCCCCCACTAGCTAGATAATCTTTG1210.11801308872438579No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC1200.11703777394153964No Hit
AGATAATACAGTTGAATTGGCAGGCACTTCTGTTGCATTACCAGCTTGTA1200.11703777394153964No Hit
GCACAAGACATCTGTCGTAGTGCAACAGGACTAAGCTCATTATTCTGTAA1200.11703777394153964No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG1190.11606245915869347No Hit
ATATTGGCTTCCGGTGTAACTGTTATTGCCTGACCAGTACCAGTGTGTGT1180.1150871443758473No Hit
ATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCC1170.11411182959300115No Hit
ATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGT1170.11411182959300115No Hit
GCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACAC1170.11411182959300115No Hit
GTACAAACCTACCTCCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCA1170.11411182959300115No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT1160.11313651481015498No Hit
AACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGAT1160.11313651481015498No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG1160.11313651481015498No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA1150.11216120002730881No Hit
AGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAA1150.11216120002730881No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA1150.11216120002730881No Hit
ATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTAAG1140.11118588524446264No Hit
CACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAAC1130.11021057046161647No Hit
ATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACT1130.11021057046161647No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA1120.10923525567877032No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT1100.10728462611307799No Hit
ATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCT1100.10728462611307799No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA1090.10630931133023183No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTC1090.10630931133023183No Hit
TAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATT1090.10630931133023183No Hit
GTACCATACCTCTATTTAGGTTGTTTAATCCTTTAATAAAGTATAAATAC1070.1043586817645395No Hit
CCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATA1070.1043586817645395No Hit
CTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAAT1070.1043586817645395No Hit
ACCAAGTACCATACCTCTATTTAGGTTGTTTAATCCTTTAATAAAGTATA1070.1043586817645395No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT1060.10338336698169333No Hit
TCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTAT1060.10338336698169333No Hit
GTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCCCCCACT1060.10338336698169333No Hit
ACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTAC1050.10240805219884716No Hit
GTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCA1050.10240805219884716No Hit
GTACAGACAACACGATGCACCACCAAAGGATTCTTGATCCATATTGGCTT1040.10143273741600102No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT1030.10045742263315487No Hit
GCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATC1030.10045742263315487No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG1030.10045742263315487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGA100.007081647145.02
TCTGGGG100.007081647145.01
CTCTAGG100.007081647145.02
CCATTGT151.2268119E-4145.06
CTAGAGG100.007081647145.04
GTCTGCC203.856057E-4108.751
GCCATTG203.856057E-4108.755
CATGAAG554.110916E-1092.272721
TGAAGAC554.110916E-1092.272723
TATTGGG451.2249784E-680.55556145
GACGGGC3800.072.57
CGGGCTG3800.072.59
AGACGGG3800.072.56
TGCCATT300.001930772172.54
CTGCCAT300.001930772172.53
GACAGTG851.6734703E-1068.23537
AAGACGG4050.068.024695
GAAGACA754.796675E-967.6666644
TAAGACG4300.067.441864
GTAAGAC4400.065.909093
Produced by FastQC (version 0.11.5)