FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-67-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-67-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218131
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA14810.6789498053921726No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA11710.5368333707726092No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT10450.47906991670143173No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA10320.47311019524964354No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG9880.45293883033589905No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC8850.40571949883326985No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC8400.3850896938078494No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA8260.3786715322443852No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG7810.3580417272189647No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA7760.35574952666058474No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC7660.35116512554382456No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT7540.34566384420371243No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG7530.3452054040920364No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC7370.3378703623052203No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC6800.31173927593968764No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT6680.3062379945995755No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT6610.3030289138178434No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT6170.2828575489040989No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC6040.2768978274523108No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA6030.2764393873406348No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA5890.2700212257771706No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT5690.26085242354365035No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC5650.2590186630969463No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC5600.25672646253856624No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT5510.2526005015334821No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA5510.2526005015334821No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT5500.2521420614218062No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT5280.2420563789649339No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG5180.2374719778481738No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC5070.23242913661973766No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC5030.23059537617303363No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT5020.2301369360613576No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA4990.22876161572632958No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA4950.22692785527962553No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA4780.21913437338113334No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT4780.21913437338113334No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG4770.21867593326945736No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT4610.21134089148264115No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC4590.21042401125928914No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT4490.20583961014252902No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT4480.20538117003085304No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4210.1930032870156007No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG4130.1893357661221926No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG4100.1879604457871646No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC4090.18750200567548858No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA4050.18566824522878453No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA4010.18383448478208048No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT3930.1801669638886724No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT3860.17695788310694033No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC3800.17420724243688424No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA3660.1677890808734201No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA3590.16458000009168802No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3570.163663119868336No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT3560.16320467975665998No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG3560.16320467975665998No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC3550.162746239644984No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA3550.162746239644984No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT3550.162746239644984No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC3540.16228779953330796No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT3510.16091247919827995No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA3470.1590787187515759No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA3450.15816183852822388No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG3370.15449431763481578No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA3370.15449431763481578No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT3310.1517436769647597No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG3260.14945147640637965No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG3250.14899303629470365No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT3230.1480761560713516No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3230.1480761560713516No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT3160.14486707528961953No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG3120.1430333148429155No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC3070.14074111428453545No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT3030.1389073538378314No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG3020.1384489137261554No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT3010.13799047361447936No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT3010.13799047361447936No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT2940.1347813928327473No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT2930.13432295272107128No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT2890.13248919227436723No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC2790.12790479115760714No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT2780.1274463510459311No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA2770.1269879109342551No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA2770.1269879109342551No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG2760.1265294708225791No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2740.12561259059922705No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG2720.12469571037587504No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA2710.12423727026419905No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC2700.12377883015252303No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA2680.122861949929171No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2660.12194506970581898No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT2630.12056974937079096No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG2610.11965286914743893No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT2560.11736066858905887No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA2540.11644378836570685No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA2530.11598534825403083No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC2530.11598534825403083No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2470.11323470758397476No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2470.11323470758397476No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA2440.11185938724894674No Hit
ACATAACCATCTATTTGTTCGCGTGGTTTGCCAAGATAATTACATCCAAT2440.11185938724894674No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2440.11185938724894674No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT2430.11140094713727071No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC2430.11140094713727071No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG2400.11002562680224269No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2390.10956718669056667No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA2370.10865030646721464No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2360.10819186635553865No Hit
ATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTT2340.10727498613218663No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG2340.10727498613218663No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT2340.10727498613218663No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT2320.1063581059088346No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT2300.10544122568548259No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA2280.10452434546213056No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA2280.10452434546213056No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG2280.10452434546213056No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2270.10406590535045454No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2270.10406590535045454No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT2270.10406590535045454No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC2260.10360746523877853No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG2260.10360746523877853No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT2250.10314902512710251No Hit
GATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACATAACAGCAGT2250.10314902512710251No Hit
AAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGT2240.10269058501542652No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG2230.1022321449037505No Hit
ACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTAATAAAC2200.10085682456872247No Hit
CCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTT2200.10085682456872247No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA2200.10085682456872247No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC2190.10039838445704645No Hit
GAATAGGAAGACAACTGAATTGGATTTGTATTCCTCCAAAATATGTAATT2190.10039838445704645No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2190.10039838445704645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA850.0136.47061
AACCCTA301.0987969E-7120.8333365
CCTAGGT1050.0110.47624
TCCTAGG1050.0110.47623
CTAGGTA1050.0110.47625
TTCCTAG1100.0105.4545442
CCCTAAT352.7544593E-7103.571427
TTAACCC352.7544593E-7103.571423
TAGGTAG1150.0100.869576
CTTAACC406.101345E-790.6252
CCTAATT406.101345E-790.6258
TGTAGGC259.376839E-487.07
TAGGCCA259.376839E-487.09
TAATGTA259.376839E-487.04
AGGTAGT1600.072.57
CTGTAAT406.6515204E-572.51
GATCACG300.001933706772.52
GTAGGCC300.001933706772.58
TACGGTG350.00356276762.1428533
ATGTAGG350.00356276762.1428536
Produced by FastQC (version 0.11.5)