FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-66-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-66-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73237
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAAGACAGTGTGGGGAGAAAGCCAGACTCAGGGTGTTTATGTTTGTG2760.3768586916449336No Hit
GTGTAGACCTGCCAACAGCGTGATTTCAGACTTCTGACTTCCAGAAGCGT2240.3058563294509606No Hit
GTGTATGTGTGTGCACATGTGATACATGAGATACACTCACAACCGCATAG1990.27172057839616587No Hit
GCCAATGCTAGGGAGAGCTGCCACTAACCACACAGAGAGGAAGGAAAATT1770.24168111746794654No Hit
GTGTGGGGAGAAAGCCAGACTCAGGGTGTTTATGTTTGTGGGTAATGACA1770.24168111746794654No Hit
GTGTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCAATGCCTCCTG1710.2334885372147958No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1510.20617993637096002No Hit
GTGTATAATAAAGTGCTAACGTAACAATAAGGTTAATAAGTTAACCCTCG1500.20481450632876824No Hit
CTCTTAGGACTCTGCCTTATGAGCTACTGATCTCTTGAGCACTCACCAAA1360.1856984857380832No Hit
GTGTACACTGACTACATACACAGTCTCTGAATGGGCAGAAGGCCAGACTA1280.1747750454005489No Hit
GTATACACCCAAAGGATTATAAATCATTCTAGTATAAAGGCACATGCATA1250.17067875527397355No Hit
CATGAAGACAGTGTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCA1150.15702445485205566No Hit
GTATATACCCAAAGGATTATAAATCATTCTACTATAAAGACACATGCACA1140.15565902480986388No Hit
ATTATACACTGATCCGAAACTTATATCTAAAATATTTGTACCAAAATCTT1100.1501973046410967No Hit
GTGTTTATGTTTGTGGGTAATGACAGTCAGCTCTGGGCTCCAGATGATGC1100.1501973046410967No Hit
GTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCAATGCCTCCTGTA1060.14473558447232956No Hit
CATGAAGACAGTGTGGTGATTCCTCAAGGATCTAGAACTAGAAATACCAC1060.14473558447232956No Hit
GCCTAAGGAGGCACTATGGAGCAGCAAGTTGGATCCCCCACTAGATGGCG1050.1433701544301378No Hit
GTATAATAAAGTGCTAACGTAACAATAAGGTTAATAAGTTAACCCTCGAA960.13108128405041167No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT950.1297158540082199No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG950.1297158540082199No Hit
CTACATACACAGTCTCTGAATGGGCAGAAGGCCAGACTAGTCTATGCCTT920.12561956388164452No Hit
GTCTCTGAATGGGCAGAAGGCCAGACTAGTCTATGCCTTTTAAACACTTC920.12561956388164452No Hit
GCCAATGCTAGGGAGGCTGCCACTAACCACACAGAGAGGAAGGAAAATTC920.12561956388164452No Hit
CATGAAGACAGTGTAGACCTGCCAACAGCGTGATTTCAGACTTCTGACTT910.12425413383945275No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC880.12015784371287737No Hit
ACTTAGAGCTGAGCCTGACATGGTCCTCAGCAAGATGTTGCAAGTTCCTA880.12015784371287737No Hit
CATATACACCATGGAATACTGTGCAGCCATAAAAAAGGATGAGTTCATGT870.11879241367068558No Hit
GTGTATATGTGCCACATTTTCTTTATCCAGTCTATTATTGATGGACATTT860.1174269836284938No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA850.116061553586302No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA820.11196526345972665No Hit
ACTTATGAGTGAGAACATGTGGTGTTTGGTTTTCTGTTCTTGTGATAGTT800.10923440337534307No Hit
CTTCTACACCATCAACAATAAATTTGCGTGTTTTCTTCAATGTTTTCTTT800.10923440337534307No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT780.10650354329095948No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA780.10650354329095948No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC780.10650354329095948No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG770.10513811324876769No Hit
GTATTCCATGGTGTATATGTGCCACATTTTCTTTATCCAGTCTATTATTG760.1037726832065759No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAC100.007077538145.000026
CATGAAG1550.0126.290331
AGACAGT2200.092.2727366
CCCCCAA259.351782E-487.02
GTACTAT259.351782E-487.03
TGTACTA259.351782E-487.02
GCTCAAT259.351782E-487.02
ATGAAGA2800.072.52
GAAGACA2850.071.228074
TGAAGAC3100.067.822583
GACCTGC554.024152E-665.9090966
GGGTAGT451.18650896E-464.444441
GTGTACT451.18650896E-464.444441
GTTCTAA350.003553316562.1428571
CATGCTA350.003553316562.1428575
AAGACAG3500.062.1428575
GCCATGC350.003553316562.1428573
TAGACCT606.742992E-660.4166684
ACAGTGT3050.059.426238
GACAGTG3500.058.07
Produced by FastQC (version 0.11.5)