FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-66-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-66-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134788
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG15421.144018755378817No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC12280.9110603317802772No Hit
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT11760.8724812297830666No Hit
CTTGTAGATCTGTTCTCTAAACGAACTAAATATTATATTAGTTTTTCTGT9250.6862628720657625No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT8710.6461999584532748No Hit
CGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATT8410.6239427842241149No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC7870.5838798706116272No Hit
CAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAG7450.5527198266908033No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA7220.5356559931151141No Hit
CTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACC6940.5148826305012315No Hit
CTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACA6840.507463572424845No Hit
CTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGAC6670.4948511736949877No Hit
ATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTTCG6430.4770454343116598No Hit
CACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCA6100.45256254265958396No Hit
ATGTTTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT5870.4354987090838947No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC5720.4243701219693148No Hit
ATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGAC5270.390984360625575No Hit
GTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCC4890.36279193993530584No Hit
GCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTG4770.35388907024364186No Hit
ATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACA4730.35092144701308725No Hit
GTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAA4720.35017954120544853No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT4600.34127667151378466No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC4350.322729026322818No Hit
GATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAAC4240.31456806243879276No Hit
AAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATG4190.31085853340059943No Hit
GCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACCAGCTCCTGGC3750.2782146778644983No Hit
CGTGAGCGCTTAACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGC3680.2730213372110277No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG3550.2633765617117251No Hit
GAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGA3520.2611508442888091No Hit
GGTATATGCAAACCTACCCAGAAAGGCCAAGGAAGTTGGAAAGCTGAAGA3490.2589251268658931No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC3320.24631272813603586No Hit
CACTTATACCTGTACCTGCTTGATAAAAACATAATGGAAAGGGAAGCCAG3280.2433451049054812No Hit
CATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCT3270.24260319909784256No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC3240.24037748167492654No Hit
ATTAAGATCAGAGCAGAACTGAAAGAGACAGAGACACAAAAAAACCTTCA3230.2396355758672879No Hit
AGCTAGCAGAAGGCAAGAAATTAAGATCAGAGCAGAACTGAAAGAGACAG3230.2396355758672879No Hit
CTTTTAAAGAAACCAGCTCCTGGCTTCATTGAATTTTGAAGGTTTTTTTG3200.2374098584443719No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC3150.2337003294061786No Hit
CTACCAGTAAGTATTTCAGAGCCCAAAACGCGAAAACAAATGGAAGAAGT3130.23221651779090127No Hit
AAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAATTA3090.22924889456034664No Hit
CGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAG3000.22257174229159865No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG2970.22034602486868268No Hit
TAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTT2950.21886221325340535No Hit
TTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTT2880.21366887259993472No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC2860.2121850609846574No Hit
CGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTT2760.20476600290827077No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC2710.20105647387007744No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2690.1995726622548001No Hit
AGATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAA2610.19363741579369084No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACTAAATATTATATTAGTTTTTCT2590.1921536041784135No Hit
GCTATATACAGGATACTTTCTAGGCAGGGCGCGGTGGCTCACTCCTGTAA2570.19066979256313618No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG2550.18918598094785885No Hit
CTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGAT2490.1847345461020269No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA2420.17954120544855626No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA2320.17212214737216963No Hit
GTCTATCAATTTTGTTGATCTTTTAAAGAAACCAGCTCCTGGCTTCATTG2320.17212214737216963No Hit
GGTATATGCAGAGGGATGGGCAGAGCTTAGAGACTGGCTGTAGATAGTAA2280.169154524141615No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG2280.169154524141615No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG2280.169154524141615No Hit
CTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAAT2220.164703089295783No Hit
GCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGA2190.16247737187286704No Hit
TTATATGCCTTTTCCCTAAAAGCTTGCTATATACAGGATACTTTCTAGGC2170.1609935602575897No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT2120.15728403121939638No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG2090.15505831379648038No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA2090.15505831379648038No Hit
CTTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGCTCACGAGTGACACCA2080.15431640798884175No Hit
ACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTA2070.15357450218120308No Hit
AACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTAT2050.15209069056592575No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT2030.15060687895064842No Hit
GGTCAGGAGTTTGAGACCAGCCGGGCCAACATGGTGAAACCCTCTCTCTA2000.14838116152773242No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCAC1970.14615544410481646No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG1960.1454135382971778No Hit
GAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTA1960.1454135382971778No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC1950.14467163248953913No Hit
GTTTGGAACTTTAATTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATT1950.14467163248953913No Hit
GAGCAGAACTGAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATG1920.14244591506662313No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC1910.14170400925898446No Hit
GTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGT1860.13799448022079117No Hit
GTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTTCGTGTTGT1860.13799448022079117No Hit
ATTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAA1840.13651066860551386No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA1820.13502685699023653No Hit
TAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGG1820.13502685699023653No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG1800.1335430453749592No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT1800.1335430453749592No Hit
GGTATATGCTTTGGGAAAACAAGTCCTGTTTTGAGATTTCCAGCTGTCCT1790.13280113956732054No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC1770.1313173279520432No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG1750.12983351633676588No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTTTGATAATGGAC1720.1276077989138499No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA1710.12686589310621124No Hit
GGTATATGCTCAGCTTCTTCTGAGGCATCAGGAAGTTTACAATCATGGCA1700.12612398729857258No Hit
CTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATT1690.1253820814909339No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG1690.1253820814909339No Hit
ACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCAC1670.12389826987565658No Hit
GTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAGC1670.12389826987565658No Hit
GAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAATTAAGATCAGAGC1660.12315636406801793No Hit
CTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATT1660.12315636406801793No Hit
TTATATGCCTTACTCCCAGATGACAGGAGTTTACAATCCGAAGACCGTCA1640.1216725524527406No Hit
CGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACCAG1630.12093064664510193No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC1630.12093064664510193No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA1620.12018874083746327No Hit
AAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCG1620.12018874083746327No Hit
TTGTAGAGCAGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGA1600.11870492922218595No Hit
GGTTATTAGTTCACCCTTATGACCTGTTTTGATTACCAAAGGTGAAAGTC1590.11796302341454729No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA1570.11647921179926995No Hit
GTGCAGGTATATGCAAACCTACCCAGAAAGGCCAAGGAAGTTGGAAAGCT1530.11351158856871531No Hit
GAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATGAAGCCAGGAG1520.11276968276107664No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG1510.11202777695343798No Hit
GAGCAGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGATGGTG1490.11054396533816066No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC1460.10831824791524469No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA1460.10831824791524469No Hit
CTCGTGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCT1450.10757634210760603No Hit
ATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAA1450.10757634210760603No Hit
CATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCG1440.10683443629996736No Hit
GCGCTTAACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAAC1440.10683443629996736No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG1420.10535062468469004No Hit
GGTATATGCTACTTAGGGGCTAGGAAGAAGACCTAGAAGAAGGAGAGGGG1390.10312490726177405No Hit
TAACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGC1380.10238300145413538No Hit
GAACTGAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATGAAGCC1380.10238300145413538No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT1360.10089918983885807No Hit
GAGCAGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCT1360.10089918983885807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACCA151.2276632E-4145.01
AGGGTTC100.0070841033145.06
CGGGGAA100.0070841033145.02
CGCACTG100.0070841033145.05
GTGCGCT100.0070841033145.0145
TTGTCTA550.0118.636372
GAACGTG451.0844815E-896.6666649
TATGCTC700.093.214285
GGTATAT2750.089.636371
GGTAAGT259.3690056E-487.0145
GGAACGT502.2537279E-887.08
TACAAAC259.3690056E-487.07
GCAGGTA1300.083.653853
GTATATG2950.083.559322
AGGTATA1350.080.555565
CAGGTAT1350.080.555564
TGCAGGT1350.080.555562
TATGCAA1100.079.090915
CAACAGC1100.072.500012
GCAAACC1200.072.58
Produced by FastQC (version 0.11.5)