FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-65-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-65-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210659
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTCAT20970.9954476191380383No Hit
GTATTACCCTGATGCCAAAATCAGAAAAAAACACATCAAAACAAAAAACA18440.8753483117265343No Hit
GTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTTTTTT11230.5330890206447386No Hit
TTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTG11080.5259685083476139No Hit
CTACCTAGGAACTGGGCCACAGAGTGAGACTCTGTCTCAAAAAAAAAAAA10990.5216962009693391No Hit
TCCATAGACAATGACAGAAGGGAGACGGACAAAGAAAGAGGCTGAGGGAG10750.5103033812939395No Hit
ATCATACACTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTT10290.48846714358275695No Hit
CCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCA9360.44431996734058354No Hit
ACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAAATTGATT8860.4205849263501678No Hit
GCTTAACCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCATT8490.4030209960172601No Hit
GTGCTGGGTGGTTTCCATTTTATTCCTCATCCTGAAGATAGCCCTGAAAG8080.3835582624051192No Hit
GTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTCATCCTGAAGATA7380.35032920501853704No Hit
TTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTACCAA6620.314251942713105No Hit
GTATAAAAATACGTCAGCAGATTATACAACCAACAAAAATCACGAAAAAA6280.29811211483962236No Hit
CTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGAGGA6020.28576989352460613No Hit
GACATGGAATGATCTCTCAGATGTGGCAAATGAAAAAAAGCAATGTATGT5880.2791240820472897No Hit
GCTCTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGA5670.2691553648313151No Hit
ATCCCCTACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAA5550.26345895499361527No Hit
TGACTACACTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATT5390.25586374187668226No Hit
CACTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTT5230.24826852875974914No Hit
ATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTA5150.24447092220128264No Hit
GTTAAATAGCTTGCCTGGTATCACAAAGCTAACATGTGATTGGCTCAGAT5140.2439962213814743No Hit
AGAAATAAGAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTA5070.2406733156428161No Hit
CTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATCATACA4910.23307810252588307No Hit
TGCTTAACCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCAT4900.23260340170607477No Hit
TGTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAA4830.22928049596741656No Hit
CTTTAATTTACTGGAAATTGATTTTTTAAAAAATGACTACACTGTAACAA4830.22928049596741656No Hit
GATTTACTTGTGTATGCTGAACCATCCTTGGATCTCTCAGACACATCCCC4830.22928049596741656No Hit
CTTACAAACTCATTCTACAAGGCCAGTATTACCCTGATGCCAAAATCAGA4750.22548288940895003No Hit
GTACTAATTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTG4710.22358408612971675No Hit
CTATAGGGGGTCGTGGATTGTGTCCTGGACACAATCCCCATTGGGGCTCT4620.21931177875144192No Hit
AAATAAGAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAA4460.21171656563450883No Hit
GATTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTACC4390.20839365989585062No Hit
TCCGTGGACCGTGAAGTTGGAAAACCCTCAATTGAATCGTTCCTTCCAGC4340.20602015579680907No Hit
GCAACAGCCTGCTCTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGG4330.20554545497700072No Hit
GTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAAG4290.2036466516977675No Hit
ACCCCACACTGCCCTTCCAGCAAAGGTAGCATCCATGTGGAGGCAAGAAT4280.20317195087795917No Hit
CTATAAACACATCTACGCAAATAAACTAGAAAATCTAGACGAAATGGATA4240.2012731475987259No Hit
CTACTCTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATC4220.2003237459591093No Hit
ATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGGTCACAAGACACT4140.19652613940064276No Hit
GTATATGCCAGCGTGAATGGTTGAGACATGTTAAGGAATCAATTAAGTTA4100.19462733612140948No Hit
TATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTCCACATGGATG4050.1922538320223679No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA4040.1917791312025596No Hit
TTTTTAAGTAGTTCAAATTCTGAAACTGTGATTTAAAAATTTTTTACAGT3810.18086101234696833No Hit
ACCTAGGAACTGGGCCACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAAA3780.17943690988754338No Hit
CCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGAGGAATTGCTTGAACC3780.17943690988754338No Hit
ACATAACTCCCTTCCCTTTCTGTGTAACAAAGGTCTCTCTGTTATCTCTT3770.17896220906773508No Hit
ATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAAAACCCT3730.1770634057885018No Hit
AATTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGG3720.17658870496869347No Hit
GTGTGGGGTAGTGGGAAGGACATAGCATCGGGGTCAGACAGAGGCAGGTT3710.17611400414888517No Hit
AAACTACACTTGAGTATAAAAATACGTCAGCAGATTATACAACCAACAAA3510.16661998775271886No Hit
GATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATCATACACTATC3510.16661998775271886No Hit
ATTTTGTGCTGACATGGAATGATCTCTCAGATGTGGCAAATGAAAAAAAG3490.16567058611310223No Hit
CTACACTTGAGTATAAAAATACGTCAGCAGATTATACAACCAACAAAAAT3440.16329708201406062No Hit
GCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCC3340.15855007381597747No Hit
GGGTAGAGGTTTGCCCCACCGTACCTCAGAACAGTACCCTAGAGCTGCAG3300.1566512705367442No Hit
CATGAGGGCAGGCCATGGGGGAGATAAGAACCACCAACCTTGGAGAACTT3270.15522716807731926No Hit
GACAATGACAGAAGGGAGACGGACAAAGAAAGAGGCTGAGGGAGATGTAA3240.1538030656178943No Hit
ATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTCCGTGGAC3220.15285366397827768No Hit
CTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTTTT3190.15142956151885273No Hit
AATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG3170.1504801598792361No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA3150.14953075823961948No Hit
TTTTTAATGTGTTGTTGAATTTTGTTTGCTAGTATTTTGTTGAGGATTTC3150.14953075823961948No Hit
GTTATTAATTGATTCATTGGCTTAAAAGGAATAAGATACATTTGCTTTTT3100.1471572541405779No Hit
CCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCATTCTACAA3100.1471572541405779No Hit
TCTTTAATGTGTAGTGTTTGTTCTTTATGAGCTCAAAGTGGCTGCTGTAG3090.14668255332076957No Hit
CTCCCAAATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTC3080.14620785250096127No Hit
AAATAGAGGAAGGAATACTTACAAACTCATTCTACAAGGCCAGTATTACC3080.14620785250096127No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC3050.14478375004153632No Hit
CTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTC3010.14288494676230307No Hit
CTACAAGGCCAGTATTACCCTGATGCCAAAATCAGAAAAAAACACATCAA3000.14241024594249474No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2940.13956204102364486No Hit
CCCAAATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTCCG2920.1386126393840282No Hit
TTCTGAAACTGTGATTTAAAAATTTTTTACAGTCAAGCATTCTGATTTTG2880.13671383610479496No Hit
CCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGGTCACA2850.13528973364537No Hit
AGATTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTAC2850.13528973364537No Hit
ATGCTGAACCATCCTTGGATCTCTCAGACACATCCCCCTTGGTCATGATT2800.13291622954632842No Hit
GAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAACATCCA2790.1324415287265201No Hit
GTTTTATTCTTGTTTTTTTTAAGTAGTTCAAATTCTGAAACTGTGATTTA2760.13101742626709517No Hit
CCCCAACATATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTCCA2750.13054272544728684No Hit
CTCCCCAACATATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTC2700.12816922134824527No Hit
GGCATAGAGAGGCTTAAAAACTTGTCTAAGATTCTCTAGCCTCTGCCCAA2700.12816922134824527No Hit
GGACATAGCATCGGGGTCAGACAGAGGCAGGTTTAGAAATCTGAGCCAAT2690.12769452052843697No Hit
GACATGGGACTGGGGAAACAAGACGAGGGAGAGAGGAAAGGAATGAATGC2680.12721981970862864No Hit
GCTATGTCCTTCCCACTACCCCACACTGCCCTTCCAGCAAAGGTAGCATC2670.1267451188888203No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2660.126270418069012No Hit
GACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAACATCCATAGAC2650.1257957172492037No Hit
GATCACGCACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATC2640.12532101642939536No Hit
CTCAGACACATCCCCCTTGGTCATGATTAATAACTTTTTTAATGTGTTGT2640.12532101642939536No Hit
GAAAGATATTGTTTATCTTGATTTTTCAGATGAGGAATCAGGGCTCAGAG2600.12342221315016211No Hit
TACACTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAA2590.1229475123303538No Hit
CTATAGAAGGCAAAGGTTAAAGAGAAGGGTGTCAGGAGAGATGTTGAGTG2580.12247281151054547No Hit
ATATTGTTTATCTTGATTTTTCAGATGAGGAATCAGGGCTCAGAGATGTT2570.12199811069073715No Hit
ATGTTAAATAGCTTGCCTGGTATCACAAAGCTAACATGTGATTGGCTCAG2570.12199811069073715No Hit
GAGCAGGTGGTGTCACTGAATCCCCTACTATGCACTTATCAAGATTTTAC2550.12104870905112054No Hit
GCTAACATGTGATTGGCTCAGATTTCTAAACCTGCCTCTGTCTGACCCCG2540.12057400823131222No Hit
ATGAAAAATAGAGGAAGGAATACTTACAAACTCATTCTACAAGGCCAGTA2540.12057400823131222No Hit
TATCAAGATTTTACTTACTTTAATTTACTGGAAATTGATTTTTTAAAAAA2530.1200993074115039No Hit
ATGTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATA2530.1200993074115039No Hit
CCTGAAGATAGCCCTGAAAGATATTGTTTATCTTGATTTTTCAGATGAGG2480.11772580331246232No Hit
ACACACTACTCTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGG2460.11677640167284568No Hit
GACAAGGGACTGGGGAAACAAGACGAGGGAGAGAGGAAAGGAATGAATGC2450.11630170085303738No Hit
CTGATATCCTGAGTCTCTCCCCAACATATCTGCACTTCCTGCAAATTGGA2430.11535229921342074No Hit
AACTAATACTGATACAGAAATCCTCAACAAAATACTAGCAAACAAAATTC2410.11440289757380412No Hit
CATAACTCCCTTCCCTTTCTGTGTAACAAAGGTCTCTCTGTTATCTCTTA2410.11440289757380412No Hit
ACTATAAACACATCTACGCAAATAAACTAGAAAATCTAGACGAAATGGAT2410.11440289757380412No Hit
ATCCTGAAGATAGCCCTGAAAGATATTGTTTATCTTGATTTTTCAGATGA2380.11297879511437917No Hit
AGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAAAACCCTCAATTGAAT2370.11250409429457084No Hit
GGGTAATAATGACTTGTTGGTGATTGTAGATATTGGGCTGTTAATTGTCA2360.11202939347476253No Hit
TTGTTACACAGAAAGGGAAGGGAGTTATGTTCAAAATCAGAATGCTTGAC2360.11202939347476253No Hit
AGATAACAGAGAGACCTTTGTTACACAGAAAGGGAAGGGAGTTATGTTCA2340.11107999183514589No Hit
GTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAAGATACATTTGCT2330.11060529101533757No Hit
CACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGG2320.11013059019552927No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2320.11013059019552927No Hit
ACTCAGGAGGCTGAGGCAGAGGAATTGCTTGAACCTAGGAGGTGGAGGCT2310.10965588937572096No Hit
CTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGGTC2300.10918118855591263No Hit
TCCTTGGATCTCTCAGACACATCCCCCTTGGTCATGATTAATAACTTTTT2290.10870648773610432No Hit
CTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAAAACC2260.10728238527667937No Hit
CTACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAAATTGA2250.10680768445687105No Hit
GTGTCACTGAATCCCCTACTATGCACTTATCAAGATTTTACTTACTTTAA2250.10680768445687105No Hit
GTCTGACCCCGATGCTATGTCCTTCCCACTACCCCACACTGCCCTTCCAG2250.10680768445687105No Hit
CAATTGAATCGTTCCTTCCAGCTCTGATATCCTGAGTCTCTCCCCAACAT2240.10633298363706276No Hit
ATCACGCACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCT2220.1053835819974461No Hit
CACTAATGCCACAGAAATACAAACTACCATCAGCGAATACTATAAACACA2210.1049088811776378No Hit
ATTTTGTGCTGACATGAGGGCAGGCCATGGGGGAGATAAGAACCACCAAC2210.1049088811776378No Hit
GTACATATACCATGTTTTCCTTATCTATTCACCTGTCGATGGACACTTAG2180.10348477871821285No Hit
TGTGTATGCTGAACCATCCTTGGATCTCTCAGACACATCCCCCTTGGTCA2180.10348477871821285No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA2180.10348477871821285No Hit
ACACTTGAGTATAAAAATACGTCAGCAGATTATACAACCAACAAAAATCA2150.1020606762587879No Hit
GTTCAAATTCTGAAACTGTGATTTAAAAATTTTTTACAGTCAAGCATTCT2140.10158597543897958No Hit
CTTTTAGGAGAACAGAGAGGTAAACATCCATAGACAATGACAGAAGGGAG2140.10158597543897958No Hit
GAAATAAGAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAA2130.10111127461917126No Hit
GTGTAACAAAGGTCTCTCTGTTATCTCTTAAATTTTGTTACATCTCCCTC2130.10111127461917126No Hit
GTATTAGTTGGGGATACTGAACTGTAGTTTTTTTTGGTTTGTTTTTTGTT2120.10063657379936294No Hit
GAACTTAACATTGTAAATTAGGAATAAGTCTGAACAAGTAAGCACCTAAA2110.10016187297955464No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTC100.0070869173145.05
TCGGCAA100.0070869173145.05
GATATAA100.0070869173145.02
GCAGGTA100.0070869173145.03
CGCACAA100.0070869173145.01
TATCGGC100.0070869173145.03
ATGGGAC403.274181E-11126.8754
TATGCAT256.4529413E-6116.05
TCGAGAG203.861775E-4108.75145
TTGTGCT2200.0105.454554
TTTGTGC2300.0100.869573
ATTGGGA800.099.68753
GACAAGG301.5965157E-596.6666641
GCTGACC301.5965157E-596.6666648
GTACTAA1150.094.565221
TACCTAA554.1472958E-1092.2727362
ACATGGG554.1472958E-1092.2727362
GGGACTG900.088.6111156
GGAACAA259.376391E-487.01
GGACTGG950.083.947377
Produced by FastQC (version 0.11.5)