FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-64-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-64-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189990
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG167988.841517974630243No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT147707.774093373335439No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG144397.599873677561978No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC141897.468287804621296No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG79464.182325385546608No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTG78554.1344281277962No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA72533.8175693457550395No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA66723.5117637770408967No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA63563.3454392336438756No Hit
GATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCA43622.2959103110690036No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG42852.255381862203274No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA38782.041160061055845No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA32231.6964050739512606No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA30691.615348176219801No Hit
TCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA26371.387967787778304No Hit
TTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC25141.323227538291489No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATA24451.286909837359861No Hit
TCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCA24211.2742775935575557No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT22931.206905626611927No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCT22121.1642718037791462No Hit
GAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGAT20241.065319227327754No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC19221.0116321911679562No Hit
CTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC18160.9558397810411075No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGT17690.9311016369282594No Hit
TGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAA15780.8305700300015791No Hit
CACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATA15200.800042107479341No Hit
GTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAG14210.7479341017948313No Hit
CAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCG14200.7474077583030686No Hit
CATGAAGACAGTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC12960.6821411653244908No Hit
GCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC12220.6431917469340491No Hit
AACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT11770.6195062898047266No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGA11720.6168745723459129No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCT10070.5300278962050634No Hit
GTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGG10000.5263434917627243No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGT9740.5126585609768936No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCAT9410.4952892257487236No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATT8780.462129585767672No Hit
CGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA8130.4279172588030949No Hit
GCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG7370.3879151534291278No Hit
GGTTCACGGTGCCTGGTATATTGGTGAACAGATGCGCAAACAGGTTCACG6880.3621243223327544No Hit
CCATGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC6630.3489657350386863No Hit
GTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC6330.33317543028580454No Hit
TGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCG6190.3258066214011264No Hit
GAATATTGGTGAACAGTTGCGCAAACAGGTTCACGGTGCCTGGAATATTG5630.2963313858624138No Hit
CAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGG5450.2868572030106848No Hit
CCAATATTCCAGGCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATT5380.2831727985683457No Hit
ACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAA5110.26896152429075215No Hit
GGTTCATGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG4790.25211853255434497No Hit
ACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTT4520.2379072582767514No Hit
CGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTG4210.22159061003210698No Hit
CTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGC3670.19316806147691984No Hit
ACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGC3140.16527185641349545No Hit
CCTGTTTGCGCATCTGCTCACCAATATTCCAGGCACCGTGAACCTGTTTG3030.1594820780041055No Hit
ATTCCAGGCACCGTGATCCTGTTTGCGCATCTGTTCACCAATATTCCAGG3020.15895573451234277No Hit
GCCTGGAATATTGGTGTACAGATGCGCAAACAGGTTCACGGTGCCTGGAA2800.14737617769356282No Hit
TATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG2750.14474446023474918No Hit
TTGGTGAACAGATGCGTAAACAGGTTCACGGTGCCTGGAATATTGGTGAA2720.14316542975946103No Hit
CACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGC2600.13684930785830834No Hit
GGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGT2530.13316490341596926No Hit
ATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAA2460.1294804989736302No Hit
CCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAAT2450.12895415548186748No Hit
AGACAGTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTG2300.12105900310542661No Hit
CCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC2170.11421653771251118No Hit
CGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCT2050.1079004158113585No Hit
CACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGA1970.10368966787725671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG100.007086373145.0145
ATAGACT100.007086373145.04
AGGTCCC100.007086373145.0145
CATGAAG2000.0141.3751
ATGAAGA2300.0122.934782
TGAAGAC2300.0122.934783
GAAGACA2400.0117.81254
AAGACAG2450.0115.4081655
ACAGTGT2600.0108.749998
AGACAGT2600.0108.749996
GACAGTG2650.0106.6981057
CAGTGTT2550.099.509819
CGCCGGG300.001933320872.5145
TATTGGG4200.053.5119145
TAATGGT701.690744E-551.785713145
CCATGAA3050.042.7868841
CATGAAC3150.041.4285742
ATGAACC3150.041.4285743
GTGTTCA3550.032.6760561
CCCCCCC200.00607430529.060-64
Produced by FastQC (version 0.11.5)