FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-64-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-64-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152546
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC48363.1701912865627415No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG42922.8135775438228467No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC35142.3035674485073354No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC22941.5038086872156595No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT16531.0836075675533936No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC16011.0495194892032567No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG13740.9007119164055432No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC11660.7643596030049952No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG11000.7210939650990521No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG10950.7178162652576927No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG10880.7132274854797896No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT10450.6850392668440994No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC10130.6640619878593998No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA9200.6030967708101163No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA8600.5637643727138044No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC7790.5106656352837833No Hit
TTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACC7720.5060768555058802No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG7700.5047657755693364No Hit
GAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTT7640.5008325357597053No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC7300.47854417683846184No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA7300.47854417683846184No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC7220.47329985709228695No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC7110.4660889174412964No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC7040.4615001376633934No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA6900.4523225781075872No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG6540.4287231392498001No Hit
GTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAG6410.42020111966226575No Hit
GATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAA6220.40774586026510035No Hit
CTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGA6220.40774586026510035No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCT6000.3933239809631194No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC5950.39004628112176No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTA5740.3762799417880508No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC5650.37038008207360407No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT5580.36579130229570095No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA5410.35464712283507926No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA5410.35464712283507926No Hit
CTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATT5370.35202496296199176No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTC5360.3513694229937199No Hit
GAATGAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTG5330.3494028030889043No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT5060.33170322394556395No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC4970.32580336423111717No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG4720.30941486502432053No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG4630.30351500530987374No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT4620.30285946534160185No Hit
ACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCAT4620.30285946534160185No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA4560.2989262255319707No Hit
CCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG4540.29761514559542696No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG4450.29171528588098017No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC4360.2858154261665334No Hit
CTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAA4300.2818821863569022No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG4240.277948946547271No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA4220.27663786661072726No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT4210.2759823266424554No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG4110.26942692695973675No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT4100.26877138699146486No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC4070.2668047670866493No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA4040.2648381471818337No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA3960.2595938274356588No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC3740.2451719481336777No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG3720.24386086819713398No Hit
CCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG3720.24386086819713398No Hit
GTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCC3670.24058316835577467No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG3640.23861654845095903No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA3620.2373054685144153No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG3530.23140560879996852No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC3530.23140560879996852No Hit
GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTT3520.2307500688316967No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA3450.2261612890537936No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA3400.22288358921243429No Hit
AAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCAC3360.22026142933934684No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG3280.21501710959317188No Hit
GGCTCATTCTGTCTTTCTTTTGAGTGTGAAGCAAAGTGTTATAAACACTA3270.21436156962490005No Hit
GAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAA3130.20518401006909393No Hit
TAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACG3110.20387293013255017No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG3060.20059523029119086No Hit
TCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAAT3060.20059523029119086No Hit
GAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTC2960.19403983060847219No Hit
GATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAA2860.18748443092575354No Hit
GAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGT2810.18420673108439423No Hit
GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGG2800.18355119111612234No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG2790.18289565114785047No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC2710.17765133140167558No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA2640.17306255162377251No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT2630.17240701165550065No Hit
GACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCT2630.17240701165550065No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT2600.17044039175068504No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG2600.17044039175068504No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC2560.16781823187759756No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC2540.16650715194105384No Hit
TCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCG2530.16585161197278198No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT2520.1651960720045101No Hit
GGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCA2510.16454053203623825No Hit
ATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAAT2450.16060729222660705No Hit
GAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTG2430.15929621229006333No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC2370.15536297248043213No Hit
GCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGG2360.15470743251216026No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA2350.1540518925438884No Hit
AGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGA2250.14749649286116973No Hit
AATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTT2250.14749649286116973No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC2230.14618541292462603No Hit
ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTA2230.14618541292462603No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT2210.14487433298808228No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT2200.14421879301981042No Hit
ACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTT2190.14356325305153855No Hit
CGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGC2180.1429077130832667No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT2140.14028555321017921No Hit
ATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAAC2120.1389744732736355No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT2120.1389744732736355No Hit
GGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTTCAATCATTCTGT2110.13831893330536363No Hit
GCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCA2100.13766339333709177No Hit
CTCGTGGACCTCTCTGAGTCTTCAAAACCATTACAACAACATCTAACAAC2070.13569677343227615No Hit
GTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTG2060.1350412334640043No Hit
CTTGTAGATCTGTTCTTTAAACGAACAAACTAAAATGTCTGATAATGGAC2050.13438569349573243No Hit
GTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACT2040.13373015352746057No Hit
CTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTG2040.13373015352746057No Hit
GTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAA2020.13241907359091684No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG2010.13176353362264495No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT1990.13045245368610126No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT1990.13045245368610126No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG1980.1297969137178294No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT1980.1297969137178294No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC1960.12848583378128564No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT1950.12783029381301378No Hit
GGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCG1950.12783029381301378No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA1940.12717475384474192No Hit
AGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGT1890.12389705400338259No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC1890.12389705400338259No Hit
ACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAA1880.12324151403511072No Hit
CCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTC1860.12193043409856699No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA1850.12127489413029512No Hit
ATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGT1840.12061935416202325No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA1830.11996381419375139No Hit
GATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGG1820.11930827422547952No Hit
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT1820.11930827422547952No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG1810.11865273425720765No Hit
CACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCA1790.11734165432066392No Hit
TCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGG1790.11734165432066392No Hit
CATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGC1780.11668611435239205No Hit
CATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCA1770.1160305743841202No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA1760.11537503441584834No Hit
ACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGG1740.1140639544793046No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA1720.11275287454276088No Hit
CTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGT1710.112097334574489No Hit
TTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGA1690.11078625463794528No Hit
GGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGC1670.10947517470140154No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT1660.10881963473312968No Hit
GATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGG1650.10816409476485782No Hit
AACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAAC1650.10816409476485782No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG1640.10750855479658594No Hit
TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAA1630.10685301482831408No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG1600.10488639492349848No Hit
ACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAG1600.10488639492349848No Hit
AAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGC1600.10488639492349848No Hit
ACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCT1600.10488639492349848No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC1580.10357531498695474No Hit
GGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGT1580.10357531498695474No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC1580.10357531498695474No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC1570.10291977501868288No Hit
TTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCG1560.10226423505041102No Hit
CTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATCAGTGCCAAGA1540.10095315511386728No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC1540.10095315511386728No Hit
TCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC1530.10029761514559543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGC100.0070850137144.999986
CACGAGT100.0070850137144.999984
ACATGTA100.0070850137144.999987
ACAGAAC100.0070850137144.999983
CCACGAG100.0070850137144.999983
GCACTCG100.0070850137144.999985
GTTCCTA2400.0141.979171
TGCTTAA351.891749E-9124.285711
ACAAACC203.859709E-4108.749993
GCTTAAC404.78758E-9108.749992
TCCTAGG3500.097.357153
TTAACCC451.085391E-896.6666644
CAAACCA554.129106E-1092.272721
TTCCTAG3950.088.1012652
CTTAACC502.2557288E-887.03
ATAGAAA259.371389E-487.03
ACCCTAA502.2557288E-887.07
TAGGTAG3950.086.265826
CCTAGGT4050.084.13584
GACCTCT451.2278633E-680.555567
Produced by FastQC (version 0.11.5)