FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-63-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-63-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243303
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA18360.7546146163425852No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA14750.606239955939713No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT13540.5565077290456756No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA13470.5536306580683346No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG11010.452522163721779No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA10670.43854781897469414No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC10390.42703953506533004No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA10380.4266285249257099No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC9820.40361195710698183No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG9570.3933367036164782No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT9070.372786196635471No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT8680.3567568011902854No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC8670.3563457910506652No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA7490.3078465945754882No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT7320.3008594222019457No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA7290.2996263917830853No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT7270.298804371503845No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA7190.2955162903868839No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC7070.2905841687114421No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA6990.28729608759448094No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT6970.28647406731524067No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA6940.28524103689638025No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC6750.2774318442435975No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG6670.2741437631266363No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC6620.2720887124285356No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG6410.2634574994965126No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT6400.26304648935689245No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT6320.25975840823993124No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT6230.25605931698334994No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT6190.2544152764248694No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT6150.25277123586638883No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA6090.25030517502866795No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC5770.2371528505608233No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC5490.22564456665145927No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT5470.224822546372219No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG5370.22071244497601758No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT5270.21660234357981611No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG4820.19810688729690962No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA4810.19769587715728945No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG4630.19029769464412688No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC4560.18742062366678586No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT4480.1841325425498247No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA4470.18372153241020456No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC4300.1767343600366621No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC4260.17509031947818152No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG4210.1730352687800808No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA4190.17221324850084052No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT4160.17098021808198008No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA4130.16974718766311966No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA4120.1693361775234995No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA4090.16810314710463906No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC4070.16728112682539878No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC4070.16728112682539878No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG4030.1656370862669182No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA4020.16522607612729806No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG4000.16440405584805778No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT3990.16399304570843765No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT3950.16234900514995704No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT3920.16111597473109662No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA3780.1553618327764146No Hit
CTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTAT3770.15495082263679447No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC3750.15412880235755416No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT3730.15330678207831386No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC3670.150840721240593No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT3670.150840721240593No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC3640.1496076908217326No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT3630.14919668068211242No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG3630.14919668068211242No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT3590.14755264012363184No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT3580.1471416299840117No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA3550.1459085995651513No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA3530.14508657928591098No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG3480.14303152858781026No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC3470.14262051844819013No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC3400.1397434474708491No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG3390.13933243733122896No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3350.13768839677274838No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC3320.13645536635388797No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT3310.1360443562142678No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA3290.1352223359350275No Hit
GATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACATAACAGCAGT3280.13481132579540736No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG3270.13440031565578722No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT3270.13440031565578722No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA3240.13316728523692678No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC3230.13275627509730664No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC3220.1323452649576865No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA3200.13152324467844623No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT3200.13152324467844623No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT3160.12987920411996562No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA3160.12987920411996562No Hit
CCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTT3130.1286461737011052No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT3130.1286461737011052No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG3120.12823516356148507No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG3100.1274131432822448No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA3000.12330304188604332No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA2980.12248102160680305No Hit
ATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTT2970.1220700114671829No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT2960.12165900132756276No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT2960.12165900132756276No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT2950.12124799118794262No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA2940.12083698104832245No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT2930.12042597090870232No Hit
CACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCA2920.12001496076908216No Hit
TTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTC2890.11878193035022173No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2880.1183709202106016No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT2850.11713788979174117No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA2840.11672687965212103No Hit
CTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTG2820.11590485937288073No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA2790.1146718289540203No Hit
GTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCC2780.11426081881440014No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG2770.11384980867478No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA2750.11302778839553972No Hit
GAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTT2740.11261677825591958No Hit
ACATAACCATCTATTTGTTCGCGTGGTTTGCCAAGATAATTACATCCAAT2740.11261677825591958No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA2730.11220576811629944No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA2730.11220576811629944No Hit
AGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATG2720.11179475797667929No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC2720.11179475797667929No Hit
GATTTGACCTTCTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGG2710.11138374783705914No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC2710.11138374783705914No Hit
TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAAT2690.11056172755781885No Hit
ACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTAATAAAC2640.10850667685971813No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT2620.10768465658047785No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC2610.10727364644085771No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT2580.10604061602199726No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2570.10562960588237712No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG2570.10562960588237712No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA2560.10521859574275697No Hit
GAATAGGAAGACAACTGAATTGGATTTGTATTCCTCCAAAATATGTAATT2560.10521859574275697No Hit
ACTAAGAAGAGATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACA2520.1035745551842764No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA2480.1019305146257958No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG2480.1019305146257958No Hit
ATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCC2480.1019305146257958No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA2470.10151950448617567No Hit
GGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCT2460.10110849434655553No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG2460.10110849434655553No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA100.007087588145.01
TTAACCC100.007087588145.03
GTTCCTA403.274181E-11126.8751
TCCTAGG554.1472958E-1092.272723
CTAGGTA554.1472958E-1092.272725
CCTAGGT554.1472958E-1092.272724
TAGGTAG554.1472958E-1092.272726
TTCCTAG608.258212E-1084.5833362
CAAATGG353.43204E-582.857143
CCGTTCA502.3009907E-672.5145
CTGCAGA300.00193397672.5145
AGGTAGT754.834874E-967.6666647
AAATTAG3000.058.05
AATTAGC902.0409061E-856.388892
TAGCTAT953.126297E-853.4210555
TTGTAGA2450.050.306122
AACACTT1750.049.7142837
GAATATA803.7262733E-545.31251
ACTTTCG657.3256006E-444.6153831
CTTGTAG2850.043.2456131
Produced by FastQC (version 0.11.5)