FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-62-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-62-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44527
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAG12302.76236889976868No Hit
CCCTAGCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGT8031.8034001841579266No Hit
CTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCT6891.5473757495452198No Hit
GTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAA4410.9904103128438926No Hit
GGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTC3800.8534147820423563No Hit
GACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAA3660.8219731848092169No Hit
CATGAAGACAGTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTG3520.7905315875760774No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG3130.7029442809980462No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA3080.6917151391290678No Hit
GTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCC2810.6310777730365845No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT2750.6176028027938105No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2740.6153569744200148No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA2660.5973903474296495No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC2430.545736294832349No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC2410.5412446380847575No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2320.5210321827205965No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2150.48285310036607No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT2090.469378130123296No Hit
CTCCCTAGCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTG2040.4581489882543176No Hit
GACTAACAGCTGATCTCTCAGCAGGAACTCTACAAGCCAGAAGAGAGTGG2010.45141150313293055No Hit
AGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATT1970.4424281896377479No Hit
GTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAA1950.43793653289015655No Hit
CAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCA1920.4311990477687695No Hit
TCCTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGG1910.4289532193949739No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC1900.42670739102117816No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1860.4177240775259955No Hit
GCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGG1840.41323242077840416No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1790.4020032789094258No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1760.3952657937880387No Hit
CATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGG1720.38628248029285606No Hit
CCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAA1680.37729916679767334No Hit
CTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGGCA1660.372807510050082No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1650.37056168167628634No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1650.37056168167628634No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT1610.3615783681811036No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT1570.3525950546859209No Hit
TTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTG1560.35034922631212523No Hit
CTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTC1540.3458575695645339No Hit
CTTATGAAGCTTGGTTTGGCTGGATATGAAATTCTGGGTTGAAAATTATT1520.34136591281694256No Hit
GTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAAT1510.33912008444314684No Hit
CTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAA1500.3368742560693512No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT1470.3301367709479642No Hit
GTTGAATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTTCCTGCTGAGA1460.3278909425741685No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1440.3233992858265771No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC1440.3233992858265771No Hit
ATATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCC1420.3189076290789858No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1400.31441597233139446No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1380.3099243155838031No Hit
GGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAGTATTT1230.27623688997686796No Hit
GTAATAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1220.2739910616030723No Hit
TCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTT1170.2627619197340939No Hit
CTTCTGGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATGGG1170.2627619197340939No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1170.2627619197340939No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG1140.2560244346127069No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG1120.2515327778651156No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG1120.2515327778651156No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1050.23581197924854586No Hit
CCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAACT1030.23132032250095447No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC1030.23132032250095447No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG1030.23132032250095447No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG1000.22458283737956747No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1000.22458283737956747No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA990.2223370090057718No Hit
TTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCT960.21559952388438475No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT960.21559952388438475No Hit
GTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATT940.21110786713679341No Hit
GCATTTGCTTGTCTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTT900.2021245536416107No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG900.2021245536416107No Hit
AAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTC890.19987872526781503No Hit
GTGTGAGAGAGACAGGCAGAGAGAGACAGAGAGAAGGAAAGAGAGAGACA880.19763289689401936No Hit
CCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATTTT880.19763289689401936No Hit
ATGTTAAGGGCAGCCAGAGAGAAAAGTCAGGTTACCCACAAGGGGAAGCC830.186403755025041No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG820.1841579266512453No Hit
GGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTG820.1841579266512453No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT810.18191209827744964No Hit
GGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAACTCTACAAG790.17742044152985828No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC790.17742044152985828No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC780.17517461315606261No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT770.17292878478226695No Hit
GAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATTTTCAA770.17292878478226695No Hit
ATAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACAT760.17068295640847128No Hit
GGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTG740.16619129966087992No Hit
GTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTA730.16394547128708425No Hit
GGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGCA710.1594538145394929No Hit
GTTAAGGGCAGCCAGAGAGAAAAGTCAGGTTACCCACAAGGGGAAGCCCA710.1594538145394929No Hit
GGCCAATATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCA710.1594538145394929No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA700.15720798616569723No Hit
ACATGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCA700.15720798616569723No Hit
ATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTC690.15496215779190156No Hit
CTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAG690.15496215779190156No Hit
TTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCA690.15496215779190156No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC680.15271632941810587No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC680.15271632941810587No Hit
CTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAA660.14822467267051453No Hit
GAACTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAA660.14822467267051453No Hit
CCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAAT650.14597884429671884No Hit
CTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAATTCTGGGTTGA650.14597884429671884No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA620.1392413591753318No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG620.1392413591753318No Hit
TTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACAA610.13699553080153615No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA610.13699553080153615No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC600.13474970242774048No Hit
ACCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAAC600.13474970242774048No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA590.13250387405394481No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG580.13025804568014912No Hit
GGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATGGGCTTCC580.13025804568014912No Hit
GTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTAC580.13025804568014912No Hit
GGTCTTTCTACCCATAGCACAAGTAGGTAGATGCCTCCATGATGAGATGA580.13025804568014912No Hit
GGGCAGGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAG580.13025804568014912No Hit
CATGAAGACAGTGTTCACCAATATTCCAGGCACCTTCTTCTCAAATATTT570.12801221730635345No Hit
ATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCCAG570.12801221730635345No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA570.12801221730635345No Hit
GTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAAGTCAGGT560.1257663889325578No Hit
ACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAA560.1257663889325578No Hit
GCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAGTATTTT560.1257663889325578No Hit
ATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAATT560.1257663889325578No Hit
TTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCCAGCCAAACCA550.1235205605587621No Hit
CTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGA550.1235205605587621No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA550.1235205605587621No Hit
GGTTACCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAG550.1235205605587621No Hit
GATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCT550.1235205605587621No Hit
GGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTT540.12127473218496643No Hit
CTCTTCTGGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATG540.12127473218496643No Hit
ATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGT540.12127473218496643No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG530.11902890381117076No Hit
CTCATACACCCTTCACCACTCTGTGGTTGGCTTGCCCTTGGCAGGCATGG530.11902890381117076No Hit
GAGAAAAGTCAGGTTACCCACAAGGGGAAGCCCATCAGACTAACAGCTGA520.11678307543737507No Hit
GATACATGGTGGGTGGGTAGATGGGTTGGTGGATGGGAAATTTTAAAGAA510.1145372470635794No Hit
CATGAAGACAGTGTGGTCATTCCTCAAAGACCTAGACACATAATTGTCAG510.1145372470635794No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC510.1145372470635794No Hit
GTTGTTGTTGTTGTTGTTGTCGTCGTTGCTGTTGTCGTTGTCGTTGTTGT500.11229141868978373No Hit
CAGCTGATCTCTCAGCAGGAACTCTACAAGCCAGAAGAGAGTGGGGGCCA500.11229141868978373No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC500.11229141868978373No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA490.11004559031598807No Hit
CTTCATAAGTGAAGGAGAAATAAAATACTTTACAGACAAGCAAATGCCGA490.11004559031598807No Hit
CAGTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTA490.11004559031598807No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC480.10779976194219237No Hit
CATCAGACTAACAGCTGATCTCTCAGCAGGAACTCTACAAGCCAGAAGAG480.10779976194219237No Hit
CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAA480.10779976194219237No Hit
TGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGAT480.10779976194219237No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA480.10779976194219237No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG470.10555393356839671No Hit
GGAAAAAATGTTAAGGGCAGCCAGAGAGAAAAGTCAGGTTACCCACAAGG470.10555393356839671No Hit
GCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGA460.10330810519460104No Hit
ATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA460.10330810519460104No Hit
GTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGATA450.10106227682080535No Hit
ATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGC450.10106227682080535No Hit
CTTTAAGAATGTTGAATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTT450.10106227682080535No Hit
CGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGC450.10106227682080535No Hit
TCTCTCAGCAGGAACTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCA450.10106227682080535No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGT151.2221833E-4145.01
GGTAGTA151.2221833E-4145.02
GTAGTAG151.2221833E-4145.03
TCCAGTG100.0070682745144.999987
TAATAAG202.108076E-6144.999982
GAATAGT100.0070682745144.999986
TGAATAG100.0070682745144.999985
CATGAAG2550.0130.784321
TGAAGAC2700.0123.518513
ATGAAGA2750.0121.272732
CCCTAGC1200.0114.7916641
ACAGTGT3000.0113.583338
AGACAGT3050.0111.721316
AGTAGAG203.841563E-4108.749995
TAGCATT1300.0105.961534
CTAGCAT1350.0102.037033
CAGTGTG3100.0100.564529
GACAGTG3400.0100.220597
AGCATTG1400.098.392865
AATAAGA301.5826408E-596.6666643
Produced by FastQC (version 0.11.5)