FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-61-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-61-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238602
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACT24471.0255572040469065No Hit
GGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA17260.723380357247634No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA12090.5067015364498202No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTG11720.491194541537791No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA11650.4882607857436233No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG10330.43293853362503254No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC9360.3922850604772802No Hit
CACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA9330.39102773656549394No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC8900.37300609382989247No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC8400.35205069530012323No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA8390.3516315873295278No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA8310.34827872356476475No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC8160.34199210400583396No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG8120.34031567212345243No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA8030.33654370038809395No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT7800.32690421706440015No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG7630.3197793815642786No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA7560.3168456257701109No Hit
CCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATA7470.31307365403475246No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACC6890.2887653917402201No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT6810.2854125279754571No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT6770.2837360960930755No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC6740.28247877218128936No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATAT6490.27200107291640474No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA6360.2665526692986647No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA6230.2611042656809247No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA6190.2594278337985432No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA6030.25272210626901703No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC6010.25188389032782627No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC5840.2447590548277047No Hit
GTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG5800.24308262294532318No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA5730.2401488671511555No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA5720.2397297591805601No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA5690.23847243526877393No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT5670.23763421932758316No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC5620.23553867947460624No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG5140.21542149688602777No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA5000.2095539852976924No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4940.2070393374741201No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA4920.20620112153292933No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT4570.19153234256209087No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT4440.18608393894435085No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT4390.1839883990913739No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA4370.18315018315018314No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA4340.18189285923839701No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA4310.18063553532661084No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT4300.18021642735601545No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG4160.17434891576768005No Hit
GTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCT4120.17267248388529854No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT4070.1705769440323216No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG4060.17015783606172621No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG4010.1680622962087493No Hit
CAATCACCTTCTTCTTCATCCTCATCTGGAGGGTAGAAAGAACAATACAT3970.16638586432636776No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA3900.16345210853220007No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG3850.16135656867922313No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA3850.16135656867922313No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTG3820.16009924476743698No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA3820.16009924476743698No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT3750.1571654889732693No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT3710.15548905709088776No Hit
CTGTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCT3700.15506994912029237No Hit
GTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAAT3680.1542317331791016No Hit
GTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCAC3660.15339351723791084No Hit
GGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAAC3630.15213619332612469No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA3620.15171708535552927No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3520.14752600564957544No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC3490.14626868173778929No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT3460.14501135782600313No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT3440.14417314188481237No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC3440.14417314188481237No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3410.14291581797302622No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT3370.14123938609064468No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC3360.1408202781200493No Hit
CGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTA3350.1404011701494539No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG3330.13956295420826315No Hit
GGTTTACCTTGGTAATCATCTTCAGTACCATACTCATATTGAGTTGATGG3310.13872473826707235No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA3300.13830563029647697No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA3270.13704830638469082No Hit
GTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTT3250.13621009044350008No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT3210.1345336585611185No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT3180.13327633464933236No Hit
GTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTT3140.13159990276695083No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT3130.13118079479635544No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT3130.13118079479635544No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT3120.13076168682576006No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA3110.13034257885516468No Hit
CTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCT3050.12782793103159235No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC3040.12740882306099696No Hit
GAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAGTTCAAAAGT3020.12657060711980622No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG3010.12615149914921084No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA2990.12531328320802004No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT2990.12531328320802004No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA2990.12531328320802004No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2990.12531328320802004No Hit
ATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATG2970.12447506726682928No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2950.12363685132563851No Hit
CATTTACTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTA2940.12321774335504314No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA2940.12321774335504314No Hit
GTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGA2930.12279863538444775No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT2930.12279863538444775No Hit
GTTTTCAAGACAACTTCCTCTGTTAACACTTCTGTGGGAAGTGTTTCTCC2920.12237952741385234No Hit
GTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTAT2900.12154131147266159No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2880.12070309553147082No Hit
AAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTG2860.11986487959028005No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCAC2850.11944577161968467No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC2790.11693112379611235No Hit
GAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTG2740.11483558394313542No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2740.11483558394313542No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT2720.11399736800194467No Hit
GGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCAT2720.11399736800194467No Hit
AAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAA2710.11357826003134928No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2710.11357826003134928No Hit
GAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAATCCCT2700.1131591520607539No Hit
GGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTT2700.1131591520607539No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT2680.11232093611956312No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2660.11148272017837235No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC2640.11064450423718158No Hit
ATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGC2640.11064450423718158No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2640.11064450423718158No Hit
CATCTGGAGGGTAGAAAGAACAATACATATGTGAAGCCAATTTAAACTCA2630.1102253962665862No Hit
GATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCG2620.10980628829599082No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2620.10980628829599082No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2610.10938718032539542No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2590.10854896438420465No Hit
AACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACTGA2580.10812985641360927No Hit
GTGTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATG2560.10729164047241851No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT2560.10729164047241851No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA2560.10729164047241851No Hit
GTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGA2550.10687253250182313No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT2550.10687253250182313No Hit
CTTCTATCACAGTGTCATCACCAAAAGTAACCTTTGTTGGTGCACCGCCT2550.10687253250182313No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2540.10645342453122775No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2510.10519610061944158No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2510.10519610061944158No Hit
TAATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAG2500.1047769926488462No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC2500.1047769926488462No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2490.10435788467825081No Hit
GGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGC2480.10393877670765543No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2470.10351966873706005No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC2440.10226234482527388No Hit
TACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTC2430.1018432368546785No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA2430.1018432368546785No Hit
CAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTA2420.10142412888408311No Hit
GCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACA2410.10100502091348773No Hit
GTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTC2400.10058591294289235No Hit
CTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTT2400.10058591294289235No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2400.10058591294289235No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2390.10016680497229698No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA2390.10016680497229698No Hit
TTCCAAAGGTTTACCTTGGTAATCATCTTCAGTACCATACTCATATTGAG2390.10016680497229698No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCC100.0070875026145.04
TATATGG100.0070875026145.05
GAGGTCT100.0070875026145.04
CGAGGTC100.0070875026145.03
GACGAGG100.0070875026145.01
TTGTCGT203.8624104E-4108.752
GAAGACA650.0100.384624
AAGACAG650.0100.384625
GACAGTG750.096.6666647
AGACAGT750.096.6666646
TGAAGAC700.093.214293
CAATTGA451.2300006E-680.555561
CAGTGTT950.076.315799
CGAACAT300.001933929772.500018
GTCCTAG300.001933929772.500011
CTAAACG300.001933929772.500013
CCCTAGC406.652802E-572.51
GCTCAAT651.3934914E-766.923082
CCTAGCA451.1920032E-464.444442
TAAACGT350.003563177362.1428574
Produced by FastQC (version 0.11.5)