FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-61-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-61-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176859
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA9990.564856750292606No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA9130.5162304434606099No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT6150.34773463606601873No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG6010.3398187256515077No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG5750.32511774916741587No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA5530.3126784613731843No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA4910.2776222866803499No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG4870.2753605979904896No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA4640.26235588802379295No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT4620.26122504367886284No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT4530.2561362441266772No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC4480.2533091332643518No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC4320.2442623785049107No Hit
TATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT4190.23691189026286474No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA4180.2363464680903997No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4160.23521562374546953No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC4070.23012682419328392No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG3900.2205146472613777No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC3890.21994922508891263No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3560.2012902933975653No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT3420.1933743829830543No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC3380.19111269429319402No Hit
GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA3340.18885100560333373No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA3290.18602389474100836No Hit
ATGCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTC3230.18263136170621797No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA3220.18206593953375289No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC3150.17810798432649738No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT3080.17415002911924188No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG3080.17415002911924188No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT2960.16736496304966103No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2940.1662341187047309No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT2920.16510327435980074No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2900.1639724300148706No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT2860.16171074132501032No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA2840.16057989698008018No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA2840.16057989698008018No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC2800.1583182082902199No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC2760.1560565196003596No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT2740.15492567525542947No Hit
GCTCTACAAGATGCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCA2710.15322940873803426No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC2660.15040229787570888No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC2640.14927145353077875No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA2530.14305180963366299No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT2480.1402246987713376No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT2470.13965927659887253No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC2460.13909385442640748No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT2460.13909385442640748No Hit
GGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACG2450.1385284322539424No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT2440.13796301008147735No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG2430.13739758790901227No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2410.13626674356408214No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT2410.13626674356408214No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG2380.1345704770466869No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA2380.1345704770466869No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT2380.1345704770466869No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT2360.13343963270175677No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT2340.13230878835682663No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA2340.13230878835682663No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2340.13230878835682663No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2290.12948167749450126No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA2270.12835083314957113No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2220.12552372228724576No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2180.1232620335973855No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG2160.12213118925245535No Hit
GTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGA2120.11986950056259507No Hit
GCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGA2070.1170423897002697No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG2060.11647696752780463No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG2060.11647696752780463No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA2010.11364985666547929No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC1970.11138816797561898No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC1950.11025732363068885No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT1930.1091264792857587No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC1920.10856105711329364No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT1900.1074302127683635No Hit
CCCTTTCGGATGGCTTAAAGGTTTATACCTTCCCAGGTAACAAACCAACC1870.1057339462509683No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT1870.1057339462509683No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT1860.10516852407850322No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG1850.10460310190603815No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC1840.10403767973357306No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT1830.10347225756110799No Hit
GTTTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT1830.10347225756110799No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA1830.10347225756110799No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA1780.10064514669878265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTGG100.0070859636145.01
TGCTTAA100.0070859636145.01
GATCACG202.1257092E-6145.01
CGCAATG100.0070859636145.01
CAAATGG301.0979238E-7120.8333363
ATCACGC256.4500528E-6116.02
TTATCGG203.8607398E-4108.752
TATCGGC203.8607398E-4108.753
ATCGGCA203.8607398E-4108.754
CTTATCG203.8607398E-4108.751
AATGGAA451.2286782E-680.555555
TAATGTA651.0899472E-555.769234
AAATTAG2650.054.716985
AATTAGC805.845959E-754.3752
CCGTTCA400.006042686354.375145
CAATATG400.006042686354.3754
GACTACC2050.053.048787
TAGCTAT858.8827437E-751.176475
TCCTAGG450.00962612448.3333322
GCAACAG450.00962612448.3333321
Produced by FastQC (version 0.11.5)