FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-60-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-60-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189120
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC21741.1495346869712353No Hit
CCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACT16720.8840947546531303No Hit
CCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT10190.5388113367174281No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG10050.5314086294416244No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA8280.43781725888324874No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC5860.30985617597292725No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG5740.3035109983079526No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG5610.29663705583756345No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA5390.2850042301184433No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT4800.25380710659898476No Hit
TCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA4710.2490482233502538No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC4670.24693316412859562No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4620.24428934010152287No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGT4520.2390016920473773No Hit
GTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGT4390.23212774957698815No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT4280.22631133671742806No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA4280.22631133671742806No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT4130.21837986463620981No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA4100.21679357021996615No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG4080.21573604060913706No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT3910.2067470389170897No Hit
GTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA3880.205160744500846No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA3870.20463197969543145No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT3810.20145939086294415No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC3700.1956429780033841No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA3670.19405668358714043No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA3620.19141285956006768No Hit
GTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCA3530.1866539763113367No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC3500.18506768189509307No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA3490.1845389170896785No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC3450.18242385786802032No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA3440.18189509306260576No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA3360.17766497461928935No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA3230.17079103214890015No Hit
AGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA3160.16708967851099832No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT3150.16656091370558376No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA3060.1618020304568528No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA3040.1607445008460237No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3000.15862944162436549No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA3000.15862944162436549No Hit
ATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC2960.15651438240270726No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT2960.15651438240270726No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2930.15492808798646363No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2920.15439932318104907No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA2900.15334179357021996No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA2880.15228426395939085No Hit
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTC2830.1496404399323181No Hit
CAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGT2820.14911167512690354No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT2800.14805414551607446No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG2790.1475253807106599No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA2780.14699661590524535No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA2770.1464678510998308No Hit
CAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTG2760.14593908629441624No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT2730.14435279187817257No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG2730.14435279187817257No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG2730.14435279187817257No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT2730.14435279187817257No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2690.1422377326565144No Hit
ATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATT2680.14170896785109982No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT2640.13959390862944163No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2620.13853637901861252No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG2570.13589255499153977No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA2540.13430626057529613No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC2520.133248730964467No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT2520.133248730964467No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC2520.133248730964467No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC2470.13060490693739424No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTG2460.13007614213197968No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2440.1290186125211506No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT2430.12848984771573604No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGA2420.1279610829103215No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT2410.12743231810490693No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT2380.12584602368866327No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT2380.12584602368866327No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2360.12478849407783418No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT2310.12214467005076143No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2300.12161590524534688No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2290.12108714043993231No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA2270.12002961082910321No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA2260.11950084602368866No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA2250.11897208121827413No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2240.11844331641285956No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC2230.117914551607445No Hit
GATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGC2220.11738578680203046No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG2210.1168570219966159No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT2210.1168570219966159No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG2180.11527072758037225No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2150.1136844331641286No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT2150.1136844331641286No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT2150.1136844331641286No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA2150.1136844331641286No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA2130.1126269035532995No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAA2130.1126269035532995No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT2130.1126269035532995No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2110.11156937394247038No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2110.11156937394247038No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2100.11104060913705585No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2090.11051184433164128No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC2070.10945431472081218No Hit
ATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGAT2070.10945431472081218No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2050.10839678510998307No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2050.10839678510998307No Hit
ACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGT2050.10839678510998307No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2050.10839678510998307No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2040.10786802030456853No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2030.10733925549915398No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC2030.10733925549915398No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC2030.10733925549915398No Hit
GCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC2020.10681049069373942No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA2010.10628172588832488No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2010.10628172588832488No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT2000.10575296108291032No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA1930.10205160744500845No Hit
CACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAAC1930.10205160744500845No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA1930.10205160744500845No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT1920.10152284263959391No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC1920.10152284263959391No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT1910.10099407783417935No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC100.007086348145.02
GTCTCAA151.2284408E-4145.01
CAACCGC100.007086348145.01
TCAATGT301.09821485E-7120.8333364
GCTCAAT301.09821485E-7120.8333362
GAAGACA850.0110.882354
CGCCTAA203.8611572E-4108.751
AAGACAG850.0102.3529365
CCCTAGC501.9463187E-10101.51
AGGGCTG259.374897E-487.07
TAGCATT608.240022E-1084.5833364
GACAGTG950.083.9473657
TGAAGAC1050.082.857143
CAGTGTT1000.079.759
CATGAAG1100.079.090911
CCTAGCA651.5552359E-978.076922
CTAGCAT651.5552359E-978.076923
AGACAGT1050.075.952386
CGAACAT300.001933307372.59
CTAAACG300.001933307372.54
Produced by FastQC (version 0.11.5)