FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-58-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-58-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143460
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA4560.3178586365537432No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG4530.31576746131325806No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4310.3004321762163669No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT4180.29137041684093123No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC4120.28718806635996097No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC4050.2823086574654956No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT4040.28161159905200056No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG4010.27952042381151543No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA3930.27394395650355496No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA3800.26488219712811933No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA3460.2411822110692876No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT3300.2300292764533668No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT3290.22933221803987172No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT3180.22166457549142618No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA3160.2202704586644361No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT3110.21678516659696082No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC3070.2139969329429806No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC2930.2042381151540499No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA2840.19796458943259443No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC2810.1958734141921093No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA2760.19238812212463405No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA2750.19169106371113898No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG2700.18820577164366373No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA2690.18750871323016868No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA2690.18750871323016868No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2640.18402342116269343No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA2590.18053812909521819No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA2590.18053812909521819No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2570.17914401226822807No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT2570.17914401226822807No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA2540.17705283702774294No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG2530.1763557786142479No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2520.17565872020075282No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA2510.17496166178725778No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG2500.17426460337376273No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA2490.1735675449602677No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC2450.17077931130628746No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT2440.1700822528927924No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2390.16659696082531716No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG2350.16380872717133696No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT2340.16311166875784192No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC2340.16311166875784192No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT2330.16241461034434684No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT2320.1617175519308518No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT2310.16102049351735676No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2300.1603234351038617No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT2280.1589293182768716No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT2280.1589293182768716No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2240.1561410846228914No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2230.15544402620939635No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG2220.1547469677959013No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2200.15335285096891121No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA2170.15126167572842605No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2150.14986755890143594No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA2130.14847344207444582No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA2130.14847344207444582No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2110.14707932524745573No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG2050.14289697476648544No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA2040.14219991635299037No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT2040.14219991635299037No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT2030.14150285793949532No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2030.14150285793949532No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT1980.13801756587202008No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG1960.13662344904503No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1960.13662344904503No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT1950.13592639063153492No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT1940.13522933221803987No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC1930.13453227380454483No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG1930.13453227380454483No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG1920.13383521539104978No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC1920.13383521539104978No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT1870.13034992332357453No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG1830.12756168966959433No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT1810.12616757284260421No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA1810.12616757284260421No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC1800.12547051442910914No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT1800.12547051442910914No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT1790.1247734560156141No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG1790.1247734560156141No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT1790.1247734560156141No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA1780.12407639760211905No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC1760.12268228077512895No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA1760.12268228077512895No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT1760.12268228077512895No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA1750.1219852223616339No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT1740.12128816394813885No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC1740.12128816394813885No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA1730.1205911055346438No Hit
CTTTAAGTGGTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATG1720.11989404712114875No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA1720.11989404712114875No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA1710.1191969887076537No Hit
TATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCT1710.1191969887076537No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA1700.11849993029415865No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA1700.11849993029415865No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA1690.1178028718806636No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA1690.1178028718806636No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC1670.1164087550536735No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG1670.1164087550536735No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT1660.11571169664017844No Hit
CACCAGACTCATCAAATAAGTAGTATGTAGCCATACTCCACTCATCTAAA1650.1150146382266834No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA1650.1150146382266834No Hit
GTTAGTAGCCTTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGT1650.1150146382266834No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA1630.1136205213996933No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA1630.1136205213996933No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA1610.11222640457270319No Hit
TAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCT1600.11152934615920815No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA1580.11013522933221805No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG1580.11013522933221805No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT1570.10943817091872299No Hit
CGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTA1560.10874111250522794No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC1560.10874111250522794No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT1540.10734699567823784No Hit
CATTTACTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTA1540.10734699567823784No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG1530.1066499372647428No Hit
GTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTGACTATC1520.10595287885124774No Hit
CATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTTCT1490.10386170361076257No Hit
AAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAA1490.10386170361076257No Hit
GTTTGTTACCATCATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTT1480.10316464519726752No Hit
GTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTT1480.10316464519726752No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA1470.10246758678377248No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA1460.10177052837027742No Hit
CATCTGGAGGGTAGAAAGAACAATACATATGTGAAGCCAATTTAAACTCA1460.10177052837027742No Hit
TTTTTAATGTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATT1450.10107346995678237No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGT100.0070845773145.02
CATTATG100.0070845773145.06
GTCTCAA100.0070845773145.01
GAATATT202.124325E-6145.01
AATATTG202.124325E-6145.02
GAAGACA501.8189894E-12116.04
CGAACAT256.4458654E-6116.08
GACAGTG553.6379788E-12105.4545447
AAGACAG553.6379788E-12105.4545445
TGAAGAC553.6379788E-12105.4545443
GAGGCGG352.7496026E-7103.571429
GTAGAGG352.7496026E-7103.571426
AGAGGCG352.7496026E-7103.571428
GGGTAGT301.59477E-596.6666641
AGACAGT607.2759576E-1296.6666646
TCATGTA259.370247E-487.02
TCCCTAG502.2548193E-887.02
TAAACGA353.4272292E-582.857144
AACGAAC353.4272292E-582.857146
CTAAACG353.4272292E-582.857143
Produced by FastQC (version 0.11.5)