FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-57-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-57-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215497
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG7280.33782372840457175No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT6780.31462154925590613No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT6510.30209237251562665No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC6440.2988440674348135No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA6330.29373958802210703No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC5880.272857626788308No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA5780.2682171909585748No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT5770.2677531473756015No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT5720.26543292946073493No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT5440.2524397091374822No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG5230.24269479389504262No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA4980.2310937043207098No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT4740.2199566583293503No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC4690.21763644041448374No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC4590.2129960045847506No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA4590.2129960045847506No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC4260.19768256634663128No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG4240.19675447918068464No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT4190.1944342612658181No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA4170.19350617409987145No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT4140.19211404335095153No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA4060.18840169468716503No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC4020.18654552035527178No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA4020.18654552035527178No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA4010.18608147677229844No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA4010.18608147677229844No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3970.1842253024404052No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA3900.18097699735959202No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA3880.18004891019364538No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA3800.17633656152985888No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA3750.17401634361499232No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA3660.16983995136823252No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA3600.16705568987039263No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA3540.16427142837255274No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG3500.16241525404065948No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT3470.16102312329173957No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA3440.15963099254281962No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT3390.15731077462795306No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG3380.15684673104497973No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA3370.15638268746200643No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT3370.15638268746200643No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC3370.15638268746200643No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA3350.15545460029605981No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT3330.15452651313011317No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC3320.15406246954713987No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG3270.1517422516322733No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT3230.14988607730038006No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG3220.14942203371740675No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA3190.1480299029684868No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3170.14710181580254017No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT3170.14710181580254017No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT3150.14617372863659356No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC3140.14570968505362025No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA3110.1443175543047003No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT3070.14246137997280703No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT3070.14246137997280703No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG3060.14199733638983375No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG3010.13967711847496717No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2950.13689285697712728No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC2930.13596476981118066No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2930.13596476981118066No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT2900.13457263906226072No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2890.1341085954792874No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2880.1336445518963141No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2850.13225242114739416No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2840.13178837756442086No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2820.13086029039847422No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2810.1303962468155009No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT2800.1299322032325276No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2760.12807602890063435No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2760.12807602890063435No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2730.1266838981517144No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT2730.1266838981517144No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT2720.1262198545687411No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA2720.1262198545687411No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA2710.1257558109857678No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG2700.12529176740279446No Hit
GTTAGTAGCCTTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGT2670.12389963665387453No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2640.1225075059049546No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA2640.1225075059049546No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2610.12111537515603465No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT2560.1187951572411681No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA2560.1187951572411681No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT2550.11833111365819478No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2550.11833111365819478No Hit
CATTTACTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTA2530.11740302649224815No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2510.11647493932630153No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT2510.11647493932630153No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA2500.11601089574332822No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA2500.11601089574332822No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT2490.1155468521603549No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2490.1155468521603549No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2480.11508280857738158No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC2480.11508280857738158No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC2470.11461876499440829No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2470.11461876499440829No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2470.11461876499440829No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA2450.11369067782846165No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC2450.11369067782846165No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT2420.11229854707954172No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG2420.11229854707954172No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC2420.11229854707954172No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT2390.11090641633062179No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2390.11090641633062179No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG2390.11090641633062179No Hit
TTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGCCACAACACAGG2380.11044237274764847No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT2360.10951428558170183No Hit
GTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGA2360.10951428558170183No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC2350.10905024199872851No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC2350.10905024199872851No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA2340.10858619841575522No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC2330.1081221548327819No Hit
CTTCTATCACAGTGTCATCACCAAAAGTAACCTTTGTTGGTGCACCGCCT2310.10719406766683526No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG2280.10580193691791533No Hit
GTTTGTTACCATCATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTT2250.1044098061689954No Hit
ACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAA2230.10348171900304876No Hit
TTTTTAATGTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATT2230.10348171900304876No Hit
AAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAA2210.10255363183710214No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC2210.10255363183710214No Hit
CACCAGACTCATCAAATAAGTAGTATGTAGCCATACTCCACTCATCTAAA2200.10208958825412882No Hit
CCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTAC2190.1016255446711555No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA2180.1011615010881822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATATT500.0130.51
GAAGACA700.0124.2857064
AAGACAG700.0124.2857065
TCCCTAG351.895387E-9124.2857062
AATATTG550.0118.636372
TGAAGAC800.0108.753
GGGTAGT553.6379788E-12105.454551
AGACAGT850.0102.3529366
GACAGTG900.096.6666647
CATTCAG259.376682E-487.02
GCATTCA259.376682E-487.01
TCAATGT554.3779437E-879.090914
GCTCAAT554.3779437E-879.090912
GGTGAAC950.076.315798
GGTAGTA809.822543E-1172.52
CTGCCAT300.001933674672.53
CAGTGTT1200.072.59
CCCTAGC651.3928548E-766.923071
GTAGAGG902.8012437E-1064.444446
AGAATAC350.003562708462.1428533
Produced by FastQC (version 0.11.5)