FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-57-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-57-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278771
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA11340.40678549777415873No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT10070.36122839176241434No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA8230.29522439565091063No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT7150.2564829196724193No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA6040.21666529158341435No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT5950.2134368352518734No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG5830.20913222680981883No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG5470.19621840148365505No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC5420.19442481463279895No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT5290.18976148882057314No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA5260.18868533671005952No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC4690.1682384466103002No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA4550.1632164034279032No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC4430.1589117949858486No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC4420.15855307761567738No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC4380.15711820813499253No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG4360.15640077339465008No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA4250.15245488232276672No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC4140.14850899125088335No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG4110.1474328391403697No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT4060.14563925228951363No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC4000.14348694806848633No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG4000.14348694806848633No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA3820.13703003540540443No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC3770.13523644855454836No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG3770.13523644855454836No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC3720.13344286170369227No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA3640.13057312274232255No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG3630.13021440537215134No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3610.1294969706318089No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT3510.12590979693009674No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT3490.1251923621897543No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3490.1251923621897543No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA3370.12088775374769972No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT3360.1205290363775285No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA3320.11909416689684364No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3320.11909416689684364No Hit
TAATTATACTGCGTGAGTGCACTAAGCATGCAGCCGAGTGACAGCCACAC3240.11622442793547393No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT3230.11586571056530272No Hit
CCCTTTACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA3220.11550699319513148No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT3190.11443084108461785No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG3190.11443084108461785No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA3150.11299597160393299No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT3130.11227853686359054No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA3110.11156110212324812No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3070.11012623264256326No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA3020.10833264579170718No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC3010.10797392842153596No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2920.10474547208999502No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT2890.10366931997948137No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2820.10115829838828286No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA2810.10079958101811165No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA2800.10044086364794041No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2790.10008214627776921No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGG100.007088139145.0145
TTCCTAG351.897206E-9124.285712
TATATAC203.8631007E-4108.753
TCCTAGG404.802132E-9108.753
CCTAGGT451.0886652E-896.6666644
GTTCCTA451.0886652E-896.6666641
ATCGGCA406.10582E-790.6254
TATCGGC406.10582E-790.6253
TGCTTAA259.379597E-487.01
CCCTAAA259.379597E-487.01
TAGGTAG502.262641E-887.06
CTAGGTA502.262641E-887.05
CTATACC353.4329198E-582.857143
AATTAGC353.4329198E-582.857142
CCTATAC353.4329198E-582.857142
CCCTTTA1150.075.6521761
AAATTAG2550.073.921575
CTTAACA406.654767E-572.52
TTATCGG502.3018729E-672.52
TTATATA300.00193427372.52
Produced by FastQC (version 0.11.5)