FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-56-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-56-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21443
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1750.8161171477871567No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1150.5363055542601315No Hit
ACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCACTTTAC1120.5223149745837803No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT790.3684185981439164No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG670.3124562794385114No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA650.3031292263209439No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG610.2844751200858089No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA580.27048454040945763No Hit
CCCTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG580.27048454040945763No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA550.2564939607331064No Hit
CCCTTTACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA510.23783985449797135No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC510.23783985449797135No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA500.2331763279391876No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT500.2331763279391876No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA470.21918574826283638No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT460.2145222217040526No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG430.20053164202770132No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT430.20053164202770132No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG430.20053164202770132No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA420.19586811546891758No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT410.19120458891013384No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG400.1865410623513501No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG400.1865410623513501No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT400.1865410623513501No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC390.18187753579256635No Hit
GGGTAACACCACTGCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCA390.18187753579256635No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC390.18187753579256635No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG380.17721400923378258No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT380.17721400923378258No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA370.17255048267499884No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC370.17255048267499884No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT370.17255048267499884No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT370.17255048267499884No Hit
GTATAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCC360.16788695611621507No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT360.16788695611621507No Hit
TAATTATACTGCGTGAGTGCACTAAGCATGCAGCCGAGTGACAGCCACAC360.16788695611621507No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT350.16322342955743133No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC350.16322342955743133No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG350.16322342955743133No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA340.15855990299864758No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC340.15855990299864758No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT340.15855990299864758No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT320.14923284988108007No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG310.14456932332229633No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT300.13990579676351256No Hit
GGGTAATTGAGTTCTGGTTGTAAGATTAACACACTGACTAGAGACTAGTG300.13990579676351256No Hit
CCTTGCGGTAAGCCACTGGTATTTCGCCCACATGAGGGACAAGGACACCA300.13990579676351256No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT300.13990579676351256No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC290.13524227020472881No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT290.13524227020472881No Hit
AGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTA290.13524227020472881No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA280.13057874364594507No Hit
ACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGT280.13057874364594507No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT280.13057874364594507No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC270.1259152170871613No Hit
GTAGTATCTTTAGCTAAGAGAATGTCATTGTGTAACTGGACACATTGAGC270.1259152170871613No Hit
GGGTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTG260.12125169052837756No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC260.12125169052837756No Hit
GCTTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT260.12125169052837756No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC250.1165881639695938No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG250.1165881639695938No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG250.1165881639695938No Hit
GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA250.1165881639695938No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG240.11192463741081006No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC240.11192463741081006No Hit
GTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAAT240.11192463741081006No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA240.11192463741081006No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC240.11192463741081006No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT240.11192463741081006No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG240.11192463741081006No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT240.11192463741081006No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA240.11192463741081006No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG240.11192463741081006No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA240.11192463741081006No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA230.1072611108520263No Hit
GCTTAATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG230.1072611108520263No Hit
GTATAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC230.1072611108520263No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC230.1072611108520263No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC230.1072611108520263No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC220.10259758429324253No Hit
GTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCC220.10259758429324253No Hit
ATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTAT220.10259758429324253No Hit
GTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAA220.10259758429324253No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG220.10259758429324253No Hit
GAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACA220.10259758429324253No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA220.10259758429324253No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT220.10259758429324253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACC100.007042793144.999982
ATAACCT100.007042793144.999983
GTCATGC203.814003E-4108.749996
AAATTAG502.1638698E-887.05
TATGTCA259.2608243E-487.03
AGATATA259.2608243E-487.0145
TAATTCA300.001909869672.54
ATTCAAT300.001909869672.56
ATAAATT651.3338467E-766.923073
GGCATAG350.003519009662.1428576
TTCAATT350.003519009662.1428577
ATTAGGC350.003519009662.1428572
AATTCAA350.003519009662.1428575
TCAATTT350.003519009662.1428578
ATATGAT350.003519009662.142857145
TTAGGCA350.003519009662.1428573
GATTAGG350.003519009662.1428571
GCATAGC350.003519009662.1428577
TAGGCAT350.003519009662.1428574
TTATTGG501.9659227E-458.04
Produced by FastQC (version 0.11.5)