FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-55-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-55-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156043
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA15380.9856257570028775No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT6500.41655184788808214No Hit
ACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCACTTTAC6390.40950250892382234No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT5500.35246694821299257No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG5490.3518260992162417No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA4800.3076075184404299No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT4720.3024807264664227No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG4720.3024807264664227No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA4390.2813327095736432No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG4180.26787488064187437No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT4170.26723403164512344No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA4080.2614663906743654No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA3780.24224092077183854No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT3720.23839582679133314No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA3550.22750139384656795No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT3530.22621969585306612No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG3500.2242971488628135No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT3490.22365629986606256No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC3280.21019847093429375No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT3230.2069942259505393No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC3180.2037899809667848No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA3170.2031491319700339No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT3120.19994488698627944No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC3030.19417724601552136No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT3020.19353639701877048No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2980.1909730010317669No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG2930.1877687560480124No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC2840.18200111507725433No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2840.18200111507725433No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA2780.17815602109674897No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA2780.17815602109674897No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2780.17815602109674897No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2780.17815602109674897No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2750.17623347410649629No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT2630.16854328614548553No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC2630.16854328614548553No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC2560.16405734316822929No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG2540.16277564517472748No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC2530.16213479617797658No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA2490.159571400190973No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT2480.1589305511942221No Hit
GCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCT2420.15508545721371672No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA2420.15508545721371672No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA2360.15124036323321136No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA2290.1467544202559551No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA2280.14611357125920418No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT2270.1454727222624533No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2270.1454727222624533No Hit
GTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATT2250.1441910242689515No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG2240.1435501752722006No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT2200.14098677928519704No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA2140.13714168530469167No Hit
GTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAA2140.13714168530469167No Hit
AGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTA2120.13585998731118987No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC2090.13393744032093718No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG2080.13329659132418628No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC2060.1320148933306845No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT2060.1320148933306845No Hit
AATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAGTT2050.1313740443339336No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG2050.1313740443339336No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA2050.1313740443339336No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT2040.1307331953371827No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT2040.1307331953371827No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT2020.12945149734368092No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC2010.12881064834693No Hit
ATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCG2000.1281697993501791No Hit
GGGTAATTGAGTTCTGGTTGTAAGATTAACACACTGACTAGAGACTAGTG2000.1281697993501791No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT1980.12688810135667733No Hit
GTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAAAC1960.12560640336317552No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG1930.12368385637292285No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT1930.12368385637292285No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG1930.12368385637292285No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC1900.12176130938267016No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG1900.12176130938267016No Hit
GATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACA1890.12112046038591927No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT1880.12047961138916838No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA1880.12047961138916838No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA1870.11983876239241746No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC1850.11855706439891568No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA1830.11727536640541389No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG1830.11727536640541389No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT1820.116634517408663No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC1820.116634517408663No Hit
GGGTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTG1810.11599366841191211No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA1800.1153528194151612No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1800.1153528194151612No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA1800.1153528194151612No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA1790.1147119704184103No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG1780.11407112142165941No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT1780.11407112142165941No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT1770.11343027242490852No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT1770.11343027242490852No Hit
GGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCAT1770.11343027242490852No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA1750.11214857443140674No Hit
GTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTT1740.11150772543465583No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA1740.11150772543465583No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT1730.11086687643790494No Hit
ATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAAA1710.10958517844440316No Hit
ATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTAT1680.10766263145415046No Hit
GTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCT1660.10638093346064866No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT1650.10574008446389777No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC1640.10509923546714688No Hit
GAGTAAGACTACTGATGTGCACTTTACATCTGACATTTTAGACTGTACAG1640.10509923546714688No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG1620.10381753747364508No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT1620.10381753747364508No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT1610.10317668847689418No Hit
GTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATATCCAACCATG1600.10253583948014329No Hit
ACTCAACACACATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTA1600.10253583948014329No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA1600.10253583948014329No Hit
CTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTTG1600.10253583948014329No Hit
CCCTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1600.10253583948014329No Hit
TGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAA1590.1018949904833924No Hit
CCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTT1590.1018949904833924No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC1590.1018949904833924No Hit
CCTTAGAAACTACAGATAAATCTTGGGATTTTATTATTTCAACAAAATCA1580.1012541414866415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATT100.007085167145.04
CTATACC100.007085167145.03
TCTATAC100.007085167145.02
GATTGCA100.007085167145.07
CTGAACA100.007085167145.09
GCTTAAT202.1249143E-6145.01
TGCTTAA301.09730536E-7120.8333361
CCTTATA203.859876E-4108.752
CCCTTAT203.859876E-4108.751
TTAATAC301.59521E-596.6666643
AAATTAG3100.095.887095
CTTAACC451.2279961E-680.555563
TTAACCC451.2279961E-680.555564
TAGCTAT554.370122E-879.0909045
ATAAATT4150.073.373493
ATAGGCG300.001932670472.57
AATTAGC607.9871825E-872.52
AACCCTA502.2971144E-672.56
TTAAGCC300.001932670472.54
CTGTTTA300.001932670472.56
Produced by FastQC (version 0.11.5)