FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n02_CV-54-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n02_CV-54-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132064
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA12500.946510782650836No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT5020.3801187303125757No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT4760.3604313060334383No Hit
ACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCACTTTAC4710.356645262902835No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA4080.30894111945723285No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG3760.2847104434213715No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT3570.27032347952507874No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG3440.26047976738551004No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG3360.25442209837654467No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT3280.24836442936757935No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG3200.24230676035861398No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA3140.23776350860189No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA3110.235491882723528No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC2980.22564817058395928No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT2720.2059607463048219No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA2710.20520353767870125No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA2690.2036891204264599No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT2660.20141749454809788No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC2650.20066028592197724No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2630.19914586866973588No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA2630.19914586866973588No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG2570.19460261691301187No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT2570.19460261691301187No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT2550.19308819966077054No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC2400.18173007026896051No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2390.18097286164283985No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2390.18097286164283985No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2330.17642960988611583No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT2300.1741579840077538No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2280.17264356675551248No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA2230.16885752362490913No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT2210.1673431063726678No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA2200.16658589774654714No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC2170.16431427186818512No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG2170.16431427186818512No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT2150.1627998546159438No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT2110.1597710201114611No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2100.15901381148534044No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA2050.1552277683547371No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC2030.15371335110249576No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2020.1529561424763751No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT2010.15219893385025443No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC2010.15219893385025443No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA1990.15068451659801307No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC1990.15068451659801307No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT1970.14917009934577175No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC1960.14841289071965108No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG1860.14084080445844438No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA1860.14084080445844438No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT1840.13932638720620305No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT1830.13856917858008239No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA1820.13781196995396172No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA1820.13781196995396172No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT1780.13478313544947904No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA1770.13402592682335837No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA1770.13402592682335837No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC1760.1332687181972377No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT1740.13175430094499635No Hit
AGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTA1740.13175430094499635No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT1740.13175430094499635No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA1720.13023988369275502No Hit
GTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATT1700.1287254664405137No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG1690.12796825781439303No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA1680.12721104918827233No Hit
GCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCT1680.12721104918827233No Hit
GATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACA1670.12645384056215167No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA1660.125696631936031No Hit
GTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAAAC1660.125696631936031No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA1660.125696631936031No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG1650.12493942330991034No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT1650.12493942330991034No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT1650.12493942330991034No Hit
ATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCG1610.12191058880542767No Hit
GGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCAT1600.12115338017930699No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG1590.12039617155318633No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC1550.11736733704870365No Hit
CTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTTG1550.11736733704870365No Hit
ATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAAA1540.11661012842258299No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT1540.11661012842258299No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA1520.11509571117034165No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT1520.11509571117034165No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA1510.11433850254422098No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG1500.1135812939181003No Hit
ATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCA1500.1135812939181003No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG1490.11282408529197964No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA1480.11206687666585897No Hit
AATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAGTT1470.1113096680397383No Hit
GTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCT1470.1113096680397383No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC1450.10979525078749697No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA1420.10752362490913496No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG1420.10752362490913496No Hit
CTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTAT1420.10752362490913496No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT1400.10600920765689362No Hit
GTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAA1400.10600920765689362No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT1400.10600920765689362No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA1400.10600920765689362No Hit
GAATTAGTGTATGCAGGGGGTAATTGAGTTCTGGTTGTAAGATTAACACA1390.10525199903077295No Hit
GTCATAAGGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTT1390.10525199903077295No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA1380.10449479040465229No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG1370.10373758177853161No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA1350.10222316452629028No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT1340.1014659559001696No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG1340.1014659559001696No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1330.10070874727404894No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC1330.10070874727404894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG100.007083943145.02
GTCAACT100.007083943145.04
CTAGGTA100.007083943145.04
AACCCTA151.227608E-4144.999985
CAAATGG650.0111.538463
GACAGCT203.8585483E-4108.757
CTTAACC203.8585483E-4108.752
TTAACCC203.8585483E-4108.753
AATGGAA700.0103.5714345
CCTAATT259.368585E-487.08
CCCTAAT259.368585E-487.07
AAATTAG3100.079.516135
CCCTTTA554.364847E-879.090911
TCAAATG1050.069.0476152
TCTGAGT1400.067.3214343
ATAAATT3700.066.621633
GCGGTAA451.19003904E-464.444445
GTAAGCC451.19003904E-464.444448
TGCGGTA451.19003904E-464.444444
CGGTAAG451.19003904E-464.444446
Produced by FastQC (version 0.11.5)