FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504834
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99840.6634618835034296No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC21490.1428064490834205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAC451.2332112E-680.555323
TCGTACT300.001935955872.499792
GTGTATC1900.057.2366681
GACAGTG3350.049.775977
ATGCTCT752.5512476E-548.333198
CGTACTC450.00964018748.333193
GTATCAT2250.048.333193
TGTATCA2450.047.3467982
CAGTGTA1602.0008883E-1145.3123669
AGACAGT3700.045.0674326
CCCTTGG4100.042.43892
GCCCTTG4200.041.4284481
ACAGTGT4500.040.277668
TGGGGCT4750.035.105166
GCACACT5000.034.801056145
CTTGGGG4850.034.381344
CCTTGGG4850.034.381343
TCCTAGG850.002742463534.1175463
CTAGGTA850.002742463534.1175465
GGGCTGC4900.034.030518
Produced by FastQC (version 0.11.5)