FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222493
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG8120.36495530196455617No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT8070.3627080402529518No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC7750.3483255652986835No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA7320.32899911457888564No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG6990.31416718728229653No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC5600.25169331169969394No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA5440.24450207422255982No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT5420.24360316953791805No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA5410.24315371719559717No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA5280.23731083674542572No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG5220.23461412269150042No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT4810.21618657665634422No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT4740.2130404102600981No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG4690.21079314854849365No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA4460.20045574467511337No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG4030.18112929395531543No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC3970.17843257990139014No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3840.1725896994512187No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG3650.16405010494712194No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA3450.15506105810070428No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT3360.15101598701981636No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA3320.14921817765053283No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA3300.14831927296589106No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC3260.14652146359660753No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT3110.13977967846179432No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG3110.13977967846179432No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG3080.13843132143483164No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC3040.13663351206554813No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTAC3010.1352851550385855No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC2970.13348734566930195No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA2940.1321389886423393No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG2910.13079063161537666No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA2890.1298917269307349No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA2730.12270048945360078No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT2730.12270048945360078No Hit
ATTATACAGTTTCCTGTTCACCTTTTACAATTAATTGCCAGGAACCTAAA2690.12090268008431726No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG2670.1200037753996755No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA2640.11865541837271286No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA2610.11730706134575021No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC2610.11730706134575021No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG2610.11730706134575021No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA2580.11595870431878755No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG2560.11505979963414578No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC2520.11326199026486226No Hit
GTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCG2520.11326199026486226No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC2480.11146418089557873No Hit
CGTTTATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGA2460.11056527621093697No Hit
ACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTA2440.1096663715262952No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT2430.10921691918397433No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT2420.10876746684165345No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA2390.10741910981469081No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2380.10696965747236992No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT2370.10652020513004905No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC2360.10607075278772815No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA2340.10517184810308638No Hit
GTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCT2340.10517184810308638No Hit
CGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTG2330.1047223957607655No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA2320.10427294341844462No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2290.10292458639148197No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2290.10292458639148197No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC2290.10292458639148197No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC2260.10157622936451934No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC2230.10022787233755669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT100.007087183145.08
AACTCCC100.007087183145.05
TAGTATA100.007087183145.04
AATAGTA100.007087183145.02
CGGCTTT100.007087183145.09
TTCGGCT100.007087183145.07
CCCTAGC850.0136.470581
GGTTATA351.895387E-9124.285712
AGGTTAT351.895387E-9124.285711
AATTAGC203.8620635E-4108.752
TATAACC352.7546412E-7103.571432
TTGTTCA1150.0100.869579
TAGCTAT259.377088E-487.05
AAATTAG1050.082.857145
ATTAGCA353.431392E-582.857144
ATAACCT451.229726E-680.555553
CTTAACC300.001933757172.52
CTTGTAC451.19182645E-464.444441
TTAACCC350.0035628662.1428573
AAGCTGC450.00962936348.3333328
Produced by FastQC (version 0.11.5)