FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76064
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCT7140.9386832141354648No Hit
TCTTTAGGCAGGTCCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAAT3430.45093605384939No Hit
GTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTG2990.3930900294488852No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA2800.368111064366849No Hit
GTCTCAAGGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGC2470.32472654606647033No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2450.32209718132099285No Hit
GTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGC2430.3194678165755153No Hit
CTGTTTATGAAAAACTCAAACCCGTCCTTTACCTTCCCAGGTAACAAACC2210.2905448043752629No Hit
CTCAAGGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTA2070.2721392511569205No Hit
GGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTG2050.269509886411443No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG2010.264251156920488No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT1980.2603071098022718No Hit
GGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCA1960.2576777450567943No Hit
GCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTT1820.23927219183845183No Hit
GAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTA1770.2326987799747581No Hit
GAGTAAGACTACTGATGTGCACTTTACATCTGACATTTTAGACTGTACAG1750.23006941522928062No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1580.20771981489272193No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA1560.20509045014724445No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC1480.19457299116533447No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT1450.19062894404711822No Hit
GTCCACGAAGGATCACAGCTCCGATTACGAGTTCACTTTCTAGAAGCGGT1380.18142616743794698No Hit
ATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAG1380.18142616743794698No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG1360.1787968026924695No Hit
GAATAGTGCCATGGAGTGGCACGTTGAGAAGAATGTTAGTTTCTGGATTG1360.1787968026924695No Hit
CTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT1350.17748212031973076No Hit
GGGCTGTTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCA1320.17353807320151451No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA1310.17222339082877575No Hit
GTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGA1290.16959402608329827No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA1290.16959402608329827No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC1240.16302061421960454No Hit
TTCTAGAAGCGGTCTGGTCAGAATAGTGCCATGGAGTGGCACGTTGAGAA1240.16302061421960454No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC1210.1590765671013883No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA1190.1564472023559108No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC1170.15381783761043333No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA1150.15118847286495582No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1150.15118847286495582No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT1120.1472444257467396No Hit
ATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGAGTTCACTTTC1080.1419856962557846No Hit
ATGTAGCAACAGTGATTTCTTTAGGCAGGTCCTTGATGTCACAGCGTCCT1040.13672696676482962No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA1040.13672696676482962No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT1040.13672696676482962No Hit
GTAGCAACAGTGATTTCTTTAGGCAGGTCCTTGATGTCACAGCGTCCTAG1040.13672696676482962No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG1040.13672696676482962No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA1030.13541228439209088No Hit
GTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGAC1020.13409760201935214No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT1010.13278291964661337No Hit
GGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTG1010.13278291964661337No Hit
GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG1010.13278291964661337No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG990.1301535549011359No Hit
GTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTC970.1275241901556584No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT960.12620950778291964No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT950.1248948254101809No Hit
CTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGA950.1248948254101809No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA950.1248948254101809No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT940.12358014303744215No Hit
GTCTAACAACATCAAAAGGTGTAAATTCATCTTCTAATAAAGCACTACCC930.12226546066470341No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG930.12226546066470341No Hit
GTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAA930.12226546066470341No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC920.12095077829196467No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA920.12095077829196467No Hit
CCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCC920.12095077829196467No Hit
GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC920.12095077829196467No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA920.12095077829196467No Hit
GGTCCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGT910.11963609591922592No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC910.11963609591922592No Hit
CTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACT900.11832141354648716No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC900.11832141354648716No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT890.11700673117374842No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC890.11700673117374842No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT870.11437736642827091No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT860.11306268405553217No Hit
AGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATG840.11043331931005469No Hit
GTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCTCCGATTACGAGT840.11043331931005469No Hit
GCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGG830.10911863693731595No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG830.10911863693731595No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT820.10780395456457718No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG810.10648927219183844No Hit
GGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCG810.10648927219183844No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA800.1051745898190997No Hit
ACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAG800.1051745898190997No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA800.1051745898190997No Hit
GGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATT780.10254522507362222No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT780.10254522507362222No Hit
GTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGC770.10123054270088346No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG770.10123054270088346No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA770.10123054270088346No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA770.10123054270088346No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAT100.0070780776145.09
TCGGCTT151.225576E-4145.08
GGACTGA100.0070780776145.06
ATAGGGA100.0070780776145.04
TTTTTCG151.225576E-4145.04
GTATCAG100.0070780776145.01
CTTGGAC100.0070780776145.03
GACTGAG100.0070780776145.07
CGGCTTT151.225576E-4145.09
TAGGGAG100.0070780776145.05
TTCGGCT151.225576E-4145.07
GTCTCAA600.0132.916671
TCTCAAG700.0113.928572
TTTTCGG203.8521865E-4108.755
ATTGTAT203.8521865E-4108.751
TTTCGGC203.8521865E-4108.756
CTCAAGG800.099.68753
CAAGGGC750.096.6666645
AAATTAG700.093.214295
CAGTACA700.093.21429145
Produced by FastQC (version 0.11.5)