FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235610
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT36251.5385594838928738No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT32661.3861890412121727No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC23260.9872246509061584No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA17080.7249267857900767No Hit
GTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCC13880.5891091210050508No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG13810.5861381095878783No Hit
GTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTA12160.5161071261830992No Hit
GATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAA11720.4974321972751581No Hit
CTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTG10950.46475107168626123No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC10790.45796018844700986No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA10570.4486227239930394No Hit
ATCTATATCTGACACTACTTGTTTTCCATGAGACTCACATGGACTGTCAG9720.4125461567845168No Hit
GTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAAT8810.373923008361275No Hit
GTGTTATACACGTAGCAGACTTTAGTGGTACATAATCTATATCTGACACT8730.37052756674164933No Hit
GATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTG8480.3599168116803192No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA8470.359492381477866No Hit
GCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATG8370.3552480794533339No Hit
GTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAA8350.35439921904842747No Hit
GGTTATAAGTATCAAATTGTTTGTAAACCCACAAGCTAAAGCCAGCTGAG7910.3357242901404864No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC7760.32935783710368827No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT7620.3234158142693434No Hit
ATTCTACATCAACACCATCAACTTTTGTGTAAACAGTGTTATTAATGATA7160.3038920249564959No Hit
ATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCC7120.3021943041466831No Hit
ATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCA7100.30134544374177663No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG7000.2971011417172446No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT6870.2915835490853529No Hit
GTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTA6740.2860659564534612No Hit
GTGTTATTAATGATAGAAACTGGTACTTCACCCTGTTGTCCATCAAAGTG6650.2822460846313824No Hit
GTTATACACGTAGCAGACTTTAGTGGTACATAATCTATATCTGACACTAC6340.269088748355333No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT6330.2686643181528797No Hit
GTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCA6320.2682398879504265No Hit
ATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTT6040.25635584228173675No Hit
GTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTATAATAATTGAA5990.2542336912694707No Hit
CTACAAGGCTGTGCATCATAGAACTTCCATTCTACATCAGCTTGAGGTAC5880.2495649590424855No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA5830.24744280803021945No Hit
GGCATACACCATCTGTGAATTTGTCAGAATGTGTGGCATAAGAATAGAAT5800.2461695174228598No Hit
CACATGGACTGTCAGAGTAATAGAAAAATGGTAATTGTTTTAAATTAACA5690.24150078519587456No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA5690.24150078519587456No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA5620.2385297737787021No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA5600.23768091337379565No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC5500.23343661134926363No Hit
CGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGC5490.2330121811468104No Hit
TTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGC5480.2325877509443572No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA5460.23173889053945076No Hit
GGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAG5370.2279190187173719No Hit
GATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGT5360.22749458851491872No Hit
GTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGT5210.22112813547812063No Hit
CGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGACTATGACT5150.2185815542634014No Hit
GGTTTAATGTTGCGCTTAGCCCAAAGCTCAAATGCTACATTAACAGGTAA4960.21051738041679047No Hit
GGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATT4920.20881965960697765No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC4910.20839522940452443No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA4910.20839522940452443No Hit
CTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACA4840.20542421798735197No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG4780.20287763677263276No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT4680.1986333347481007No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA4670.1982089045456475No Hit
GCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACA4660.19778447434319427No Hit
GTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGT4620.19608675353338145No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA4610.19566232333092823No Hit
GTATTAGCAGCAATGTCCACACCCAAATTATTGAGTATTTTCACCTCTGG4550.193115742116209No Hit
GTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTA4520.19184245150884938No Hit
GTTATAAGCATCGAGATACAATCTGTACTCATTAGCATGATGTCTACAGA4420.1875981494843173No Hit
AATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGG4330.18377827766223845No Hit
CTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGC4310.18292941725733203No Hit
CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC4300.18250498705487883No Hit
CCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCT4280.1816561266499724No Hit
GCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGC4280.1816561266499724No Hit
CTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAAT4230.17953397563770637No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA4230.17953397563770637No Hit
ATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTT4230.17953397563770637No Hit
GTGTAAACAGTGTTATTAATGATAGAAACTGGTACTTCACCCTGTTGTCC4220.17910954543525318No Hit
AGTCCAATCAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCC4210.17868511523279998No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA4090.17359195280336148No Hit
AGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAA4070.1727430923984551No Hit
ATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAA4020.17062094138618905No Hit
GGGTTTAAATTCTTGTAAATTTCTACTCTGAGTAAAGTAAGTTTCAGGTA4000.16977208098128263No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA3870.16425448834939094No Hit
ACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGCAT3840.16298119774203132No Hit
ACATTAACAGGTAATGTTGTTTTATTTTCAAACAATTCTACATCAACACC3830.16255676753957812No Hit
CTTAATAGCTTTAGGGTTACCAATGTCGTGAAGAACTGGGAATTTGTCTG3810.1617079071346717No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC3750.15916132591995247No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC3740.15873689571749924No Hit
TAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTG3730.15831246551504605No Hit
GTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACA3670.1557658843003268No Hit
CAGTATTAGCAGCAATGTCCACACCCAAATTATTGAGTATTTTCACCTCT3650.1549170238954204No Hit
GCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGA3580.15194601247824796No Hit
CCTGTTGTCTATGTGATAAGAAAGTTGATGGTGTTGTCCAACAATTACCT3510.1489750010610755No Hit
CACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATC3490.1481261406561691No Hit
TCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAAC3480.14770171045371588No Hit
CTTTAGTGGTACATAATCTATATCTGACACTACTTGTTTTCCATGAGACT3450.14642841984635627No Hit
GTCTACAGACAGCACCACCTAAATTGCAACGTGTTATACACGTAGCAGAC3410.14473069903654345No Hit
CATTAGCATGATGTCTACAGACAGCACCACCTAAATTGCAACGTGTTATA3400.14430626883409023No Hit
GCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGT3380.1434574084291838No Hit
ATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATA3360.14260854802427742No Hit
CTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAA3340.141759687619371No Hit
GTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCT3300.14006196680955818No Hit
CCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGA3270.13878867620219856No Hit
CTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCG3260.13836424599974534No Hit
ATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAG3240.13751538559483892No Hit
AGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATCACAGTATTAG3170.1345443741776665No Hit
GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA3060.12987564195068121No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG3060.12987564195068121No Hit
CTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGAT3020.12817792114086837No Hit
GTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTA3000.12732906073596198No Hit
CATCAACACCATCAACTTTTGTGTAAACAGTGTTATTAATGATAGAAACT3000.12732906073596198No Hit
ATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAG2990.12690463053350878No Hit
GACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGCATAGCCTTCT2960.12563133992614914No Hit
GAATAGAATAATTCTTCTATTTTATAAGCTTTGTCACTACAAGGCTGTGC2960.12563133992614914No Hit
CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTT2930.12435804931878952No Hit
TCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTC2920.12393361911633631No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC2920.12393361911633631No Hit
TTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTA2910.12350918891388311No Hit
GTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTAT2890.12266032850897671No Hit
GTAGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATCACAGTATT2830.12011374729425746No Hit
CCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTTCGAAGGC2800.11884045668689783No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC2790.11841602648444463No Hit
GAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGT2770.11756716607953822No Hit
GTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTG2760.11714273587708501No Hit
AATTCTAAGAAATCAATTTCCATTTGACTCCTGGGTTTAAATTCTTGTAA2740.1162938754721786No Hit
GTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTG2720.11544501506727219No Hit
ATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCT2710.11502058486481898No Hit
TTTCTAAATAAGTCTACTTGACCATCAACTCTACCATCAAAAAAGACAGT2700.11459615466236578No Hit
GCTTTAGGGTTACCAATGTCGTGAAGAACTGGGAATTTGTCTGCTAATAA2690.11417172445991257No Hit
ATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACAC2670.11332286405500616No Hit
AGGTTAGATAGCACTCTAGTGTCAAATCTACAAACAATGGAATTAGCAGG2660.11289843385255294No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA2660.11289843385255294No Hit
TCTATCGACATTGCAATTCCAAAATAGGCATACACCATCTGTGAATTTGT2650.11247400365009974No Hit
GACTAAAATCTCCATAAACGATATGTTCGAAGGCATAGCCTTCTAATTTA2620.11120071304274012No Hit
CCATCAAAGTGTCCCTTATTTACAACATTAAAAGCCACATTTTCTAAACT2620.11120071304274012No Hit
GTACATAATCTATATCTGACACTACTTGTTTTCCATGAGACTCACATGGA2600.11035185263783372No Hit
GTCATAGAACAAACACCAATAGTAGATATATGTGCTGGAGCATCTCTTTT2590.10992742243538049No Hit
CACCAATAGTAGATATATGTGCTGGAGCATCTCTTTTGTAGTCCCAGATC2580.10950299223292731No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA2580.10950299223292731No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2560.10865413182802088No Hit
AATCAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAA2550.10822970162556768No Hit
CTACAGATGGTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCA2550.10822970162556768No Hit
ACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTT2530.10738084122066127No Hit
AGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAG2520.10695641101820805No Hit
GAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTG2500.10610755061330164No Hit
GTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCA2490.10568312041084843No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT2460.10440982980348883No Hit
GTTTGCGCATCTGTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAAT2460.10440982980348883No Hit
GTTTAAATTCTTGTAAATTTCTACTCTGAGTAAAGTAAGTTTCAGGTAAT2450.10398539960103562No Hit
TGTCTACAGACAGCACCACCTAAATTGCAACGTGTTATACACGTAGCAGA2440.10356096939858239No Hit
GTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATA2440.10356096939858239No Hit
CTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTG2430.1031365391961292No Hit
GCTCAAATGCTACATTAACAGGTAATGTTGTTTTATTTTCAAACAATTCT2410.10228767879122279No Hit
GTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTAT2410.10228767879122279No Hit
AGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACACTACC2380.10101438818386317No Hit
CTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACG2380.10101438818386317No Hit
GTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGT2360.10016552777895675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTC100.0070874477145.07
CACGCTA100.0070874477145.09
GTCGCGC151.2288213E-4145.09
TAATGTC151.2288213E-4145.05
GGATTAA100.0070874477145.08
CTGATTA151.2288213E-4145.07
CATCACG100.0070874477145.06
TTATACG100.0070874477145.03
TCACGCT100.0070874477145.08
TTCTCAT151.2288213E-4145.07
ACCCTCC100.0070874477145.09
AGCGTTC100.0070874477145.09
AGATTGC100.0070874477145.05
TGTCGCG151.2288213E-4145.08
AACTGAT259.37778E-487.05
TAAGGAC350.00356312162.142857145
TGTCTAT2150.060.6976746
TTTAACT6850.059.2700774
GTCTATG2250.058.0000047
TTTTTAA7150.056.783222
Produced by FastQC (version 0.11.5)