FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265391
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC20000.7536050581971506No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT15590.5874351428646789No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC10910.41109155924654567No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC10730.4043091137227713No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA10320.3888602100297297No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT9690.3651216506965195No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG9320.3511799571198722No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT9130.3440207090669993No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG8970.33799186860142205No Hit
GTGTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTA8620.3248037800829719No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC8600.3240501750247748No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG8370.31538371685550753No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA8170.30784766627353605No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA8010.3018188258079588No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA7850.29578998534238166No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG7690.2897611448768044No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG7490.2822250942948329No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG7330.2761962538292557No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC7300.27506584624195995No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA7180.2705442158927771No Hit
GTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAGC7150.26941380830548134No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG6960.2622545602526084No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC6590.24831286667596114No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA6290.23700879080300385No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA6160.2321103579247224No Hit
GAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCG6110.2302263452792295No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG6070.22871913516283518No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT5970.2249511098718495No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT5940.22382070228455375No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG5900.22231349216815943No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA5770.21741505928987798No Hit
ACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAA5700.21477744158618792No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA5340.2012125505386392No Hit
GTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATCAT5330.20083574800954063No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC5310.20008214295134352No Hit
GTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGG5290.19932853789314636No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA5210.19631411766035775No Hit
GTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAG5130.19329969742756914No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA5090.19179248731117485No Hit
CTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTT5050.19028527719478053No Hit
AAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTT5040.18990847466568195No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG4960.18689405443289336No Hit
GATTAACAGACAAGACTAATTTATGTGATGTTGATATGACATGGTCGTAA4940.1861404493746962No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG4850.18274922661280904No Hit
GTGTACATAGCCTCATAAAACTCAGGTTCCCAATACCTTGAAGTGTTATC4820.1816188190255133No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA4810.18124201649641472No Hit
GCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGC4590.17295236085624605No Hit
GGTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAG4580.1725755583271475No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC4560.17182195326895033No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA4510.16993794062345746No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT4430.16692352039066888No Hit
TTTCAGTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCA4360.16428590268697882No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT4280.16127148245419023No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC4260.16051787739599307No Hit
CTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATT4150.15637304957590875No Hit
GCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTAT4090.1541122344013173No Hit
ATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATAGCTCATACCT4070.15335862934312014No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT4060.1529818268140216No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA4050.152605024284923No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG3990.15034420911033156No Hit
ACCTGGAGCATTGCAAACATACGGATTAACAGACAAGACTAATTTATGTG3980.14996740658123298No Hit
ATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTC3930.14808339393574008No Hit
CTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTT3910.14732978887754294No Hit
TTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGG3900.14695298634844436No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC3870.14582257876114865No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC3870.14582257876114865No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG3740.14092414588286717No Hit
GTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGT3740.14092414588286717No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT3690.1390401332373743No Hit
ACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCT3660.13790972565007856No Hit
GGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAG3640.13715612059188143No Hit
GTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCAC3600.1356489104754871No Hit
GCATACTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAA3570.1345185028881914No Hit
GTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACA3550.13376489782999423No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC3500.13188088518450136No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT3480.1311272801263042No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC3480.1311272801263042No Hit
GGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGG3440.1296200700099099No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA3430.12924326748081133No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA3430.12924326748081133No Hit
CTTCACGTACAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTA3410.1284896624226142No Hit
GATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTG3400.12811285989351562No Hit
TGTCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGT3380.12735925483531846No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT3380.12735925483531846No Hit
GTTGTACCTCGGTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGT3360.12660564977712133No Hit
GTTTTTAGTTACACGATAACCAGTAAAGACATAATTTCGGTTAAGTGGTG3360.12660564977712133No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG3350.12622884724802275No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT3340.12585204471892417No Hit
GTAAACAACAGCATCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTA3300.12434483460252986No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA3300.12434483460252986No Hit
CAGTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGC3290.12396803207343128No Hit
CTGTAAGACTGTATGCGGTGTGTACATAGCCTCATAAAACTCAGGTTCCC3290.12396803207343128No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG3280.1235912295443327No Hit
GAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGG3280.1235912295443327No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG3280.1235912295443327No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA3270.12321442701523412No Hit
TGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCAT3260.12283762448613555No Hit
CTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTA3250.12246082195703697No Hit
CTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGT3250.12246082195703697No Hit
ATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCA3240.1220840194279384No Hit
GTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCA3230.12170721689883982No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA3200.1205768093115441No Hit
ACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGA3180.11982320425334694No Hit
TTTCAAAGGTGTACTCTCCTATTTGTACTTTACTGTTTTTAGTTACACGA3140.11831599413695264No Hit
TTAGCACACAATGGAAAACTAATGGGTGGTTTATGTGATTTACAATAATA3140.11831599413695264No Hit
GTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAAC3100.11680878402055835No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT3090.11643198149145977No Hit
CCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAG3070.11567837643326262No Hit
ATGTAATTCTCTGTCAGACAGCACTTCACGTACAGTAGCAATACCATAAG3060.11530157390416404No Hit
GATATGACATGGTCGTAACAGCATTTACAACATAAGAATGGTCTACGTAT3030.11417116631686833No Hit
AAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAA3020.11379436378776975No Hit
CATTTAATTTGTAAGTTGTTGTACCTCGGTAAACAACAGCATCACCATAG2980.11228715367137546No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT2940.11077994355498115No Hit
CTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTAGG2920.11002633849678399No Hit
TTCTTATGTATTGTAAGTACAAATGAAAGACATCAGCATACTCCTGATTA2920.11002633849678399No Hit
TCACCATAGTCACCTTTTTCAAAGGTGTACTCTCCTATTTGTACTTTACT2900.10927273343858683No Hit
GCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCT2890.10889593090948828No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT2880.1085191283803897No Hit
GTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTG2880.1085191283803897No Hit
ACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTAT2820.10625831320579823No Hit
TCTAACATGTGTCCTGTTAACTCATCATGTAGCTTTCTTATGTATTGTAA2780.10475110308940394No Hit
ATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCA2770.10437430056030536No Hit
AAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGT2750.10362069550210821No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2750.10362069550210821No Hit
GCATTAAAGTCAGTAACATTATCGCTACCAACACATGTATTTTTATATAA2750.10362069550210821No Hit
TGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTAT2730.10286709044391106No Hit
AGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAA2720.10249028791481248No Hit
GGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTAC2720.10249028791481248No Hit
GTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGT2680.10098307779841818No Hit
ACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTAC2670.1006062752693196No Hit
CTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTAC2660.10022947274022102No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG2660.10022947274022102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTT100.0070879473145.08
GTTCAGC100.0070879473145.09
CATCCGT100.0070879473145.07
TGTCGCG100.0070879473145.0145
GAAGGGT650.0111.538461
TCTTCAA203.862893E-4108.754
CACAGGT259.379096E-487.07
GACCTAC259.379096E-487.01
ATAGAGC900.080.555563
GATAGAG900.080.555562
TAGAGCC950.076.315794
CAGGTGC300.0019341772.59
GGTAGTA2200.072.52
AGAGCCA1050.069.0476155
TAGAGGC754.836693E-967.6666647
TGATAGA1100.065.909091
TGTAGGC554.0533923E-665.909092
TTGCTTA451.1922484E-464.444457
GTAGAGG902.8012437E-1064.444456
AGCCATG1251.8189894E-1258.0000047
Produced by FastQC (version 0.11.5)