FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194438
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAAC15160.7796829837788909No Hit
GATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAAC11970.6156204034190847No Hit
GTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATT9220.47418714448821736No Hit
AACTTATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAG8700.44744340098128965No Hit
GCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACG8490.43664304302656887No Hit
GGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTC7260.37338380357749No Hit
CTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGT6360.3270965552001152No Hit
GATATGGAACGTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTC5530.2844094261409807No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT5370.27618058198500295No Hit
CAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATG5360.27566627922525433No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA5140.2643516185107849No Hit
TTACCAGCCTGTACCAAGAAATTATGATTAGACTTACGAATGAGTAAATC4970.25560847159505856No Hit
TGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATT4660.23966508604285172No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC4650.23915078328310307No Hit
GTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTTTGCAT4530.23297915016611978No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA4520.2324648474063712No Hit
GTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATA4520.2324648474063712No Hit
CTTATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTA4330.22269309497114764No Hit
CCATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAAC4270.21960727841265595No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT4240.21806437013341012No Hit
AACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT4090.210349828737181No Hit
ACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTA4060.2088069204579352No Hit
GTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGA4040.20777831493843796No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA3910.20109237906170604No Hit
GTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAG3900.20057807630195743No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA3870.1990351680227116No Hit
GTGTAGTTGTACCACAAGTTACTTGTACCATACAACCCTCAACTTTACCA3830.19697795698371717No Hit
GTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACT3750.1928635349057283No Hit
AGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACT3740.19234923214597968No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA3730.1918349293862311No Hit
ACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAA3630.186691901788745No Hit
GTCTTGGACAGTAAACTACGTCATCAAGCCAAAGACCGTTAAGTGTAGTT3610.18566329626924777No Hit
GTATCAACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAAC3600.18514899350949918No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG3550.18257747971075614No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG3420.17589154383402422No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG3420.17589154383402422No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC3410.1753772410742756No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA3300.1697199107170409No Hit
TCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTC3270.16817700243779507No Hit
TGCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCAC3260.16766269967804648No Hit
ACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTCTGTCC3260.16766269967804648No Hit
TTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAAAG3250.16714839691829786No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT3150.16200536932081178No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT3120.16046246104156595No Hit
CTTCAGAGGTGCAGATCACATGTCTTGGACAGTAAACTACGTCATCAAGC3100.15943385552206873No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA2980.1532622224050854No Hit
GTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTT2960.15223361688558823No Hit
CCAATATTCCAGGCACCTTTTTTAGCTTTTCCTTTTGTAACTTTAAAATT2950.1517193141258396No Hit
CGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTT2930.15069070860634237No Hit
CTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGT2880.14811919480759933No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA2860.14709058928810212No Hit
GTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGA2840.1460619837686049No Hit
GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA2820.14503337824910767No Hit
TGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAAC2810.14451907548935908No Hit
GGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTG2790.14349046996986187No Hit
ACCTTAAGCTTAAGTACACAATTTTGCATAGAATGTCCAATAACCCTGAG2720.1398903506516216No Hit
GCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACA2630.13526162581388412No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC2620.1347473230541355No Hit
ATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAA2610.1342330202943869No Hit
TACTTGTACCATACAACCCTCAACTTTACCAGATGGGAATGCCATTTTTC2610.1342330202943869No Hit
ACATAAGAGAACACACAGCCTCCAAAGGCAATAGTGCGACCACCCTTACG2610.1342330202943869No Hit
ACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGT2600.1337187175346383No Hit
CTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTG2590.13320441477488967No Hit
TCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATT2560.13166150649564387No Hit
GTACACAATTTTGCATAGAATGTCCAATAACCCTGAGTTGAACATTACCA2520.12960429545664942No Hit
CAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTA2520.12960429545664942No Hit
GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATC2520.12960429545664942No Hit
CACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATA2500.1285756899371522No Hit
GCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGA2500.1285756899371522No Hit
GTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGT2470.12703278165790638No Hit
GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC2450.12600417613840917No Hit
AGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTA2440.12548987337866055No Hit
CTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTA2430.12497557061891196No Hit
GTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTAT2420.12446126785916334No Hit
CTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTT2420.12446126785916334No Hit
AGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATG2420.12446126785916334No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2410.12394696509941472No Hit
GTGAAATTGGGCCTCATAGCACATTGGTAAACACCAGATGGTGAACCATT2400.12343266233966611No Hit
ATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACAC2340.12034684578117445No Hit
ACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAG2330.11983254302142586No Hit
ACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGTTATGGC2320.11931824026167726No Hit
ATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATA2310.11880393750192864No Hit
GATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAA2310.11880393750192864No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC2300.11828963474218002No Hit
CAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAG2300.11828963474218002No Hit
TTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTC2300.11828963474218002No Hit
GAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCC2290.11777533198243141No Hit
GGGTTAAGCATGTCTTCAGAGGTGCAGATCACATGTCTTGGACAGTAAAC2280.11726102922268282No Hit
ACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAA2270.1167467264629342No Hit
CCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAAC2270.1167467264629342No Hit
GACTAAACCATGTGTCAAAATCAGCATGTTTGTTAGCAAAACAAGTATCT2270.1167467264629342No Hit
GATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGC2270.1167467264629342No Hit
CGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTA2250.11571812094343698No Hit
GTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGT2210.11366090990444254No Hit
CCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGG2170.11160369886544812No Hit
GTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTG2160.1110893961056995No Hit
GTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACT2160.1110893961056995No Hit
TTATAATCCAAACCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGA2150.11057509334595089No Hit
GTCCTGGTTGAATGCGAACAAACTTATACTTAGGTGTCTTAGGATTGGCT2150.11057509334595089No Hit
TACTCGTACCATACAACCCTCAACTTTACCAGATGGGAATGCCATTTTTC2150.11057509334595089No Hit
ATGGTGAACCATTGTAACAAGCTAACACTGAAAAAGTCTGTCCTGGTTGA2130.10954648782645368No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA2130.10954648782645368No Hit
CGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCAT2130.10954648782645368No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC2110.10851788230695646No Hit
CTTCATGAGAGTTGAAAGGCACATTTGGTTGCATTCATTTGGTGACGCAA2110.10851788230695646No Hit
GAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACTTAGGTGTCTT2110.10851788230695646No Hit
GTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCA2090.10748927678745925No Hit
CAATAACCCTGAGTTGAACATTACCAGCCTGTACCAAGAAATTATGATTA2090.10748927678745925No Hit
GTACCATACAACCCTCAACTTTACCAGATGGGAATGCCATTTTTCTAAAA2060.10594636850821341No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG2050.1054320657484648No Hit
AAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAA2040.1049177629887162No Hit
CATAGCACATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACA2020.10388915746921898No Hit
ATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTT2020.10388915746921898No Hit
CATTGGTAAACACCAGATGGTGAACCATTGTAACAAGCTAACACTGAAAA2020.10388915746921898No Hit
CCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTC2010.10337485470947036No Hit
AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA2000.10286055194972175No Hit
CACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAA2000.10286055194972175No Hit
ATTTTGCATAGAATGTCCAATAACCCTGAGTTGAACATTACCAGCCTGTA1990.10234624918997316No Hit
GACCGTTAAGTGTAGTTGTACCACAAGTTACTTGTACCATACAACCCTCA1970.10131764367047594No Hit
TGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAAT1960.10080334091072732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTGC450.0145.01
GGCTTTT100.0070865145.07
TCGTCTT100.0070865145.0145
CATTGCT550.0118.636372
ACTGAAC301.5962041E-596.6666643
AAATTAG1150.081.956535
AACGTCT903.6379788E-1272.58
ACGTCTG903.6379788E-1272.59
GAACGTC903.6379788E-1272.57
TAGCACA3050.066.557374
GCTTTGA1650.065.909091
TATAAAT1450.065.02
TTGCTTT1150.063.043484
ACTCGTA702.3246685E-762.1428572
TCGTACC702.3246685E-762.1428574
CACATTG3550.055.1408467
GATATGG4100.054.8170741
TTGATAT2050.053.048784
AACGTTA3000.050.758
TGGAACG4450.050.505625
Produced by FastQC (version 0.11.5)