FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56560
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAA3250.5746110325318246No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT2790.49328147100424324No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT2030.35891089108910895No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC1880.3323903818953324No Hit
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC1880.3323903818953324No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT1850.3270862800565771No Hit
CTCTTAACAGTATTCTTTGCTATAGTAGTCGGCATAGATGCTTTAATTCT1750.3094059405940594No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT1620.28642149929278643No Hit
GAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACA1610.28465346534653463No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT1520.26874115983026875No Hit
CTATTAACAGCAGCTAAACCATGAGTAGCAAGGGTTTTCAAACCTAATAC1280.2263083451202263No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC1270.22454031117397455No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT1240.21923620933521926No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA1240.21923620933521926No Hit
CAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTG1200.21216407355021216No Hit
GGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCAT1190.21039603960396042No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA1130.19978783592644977No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT1110.19625176803394628No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA1090.19271570014144274No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC1070.1891796322489392No Hit
CTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAA1000.1768033946251768No Hit
ACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGA990.17503536067892506No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA970.1714992927864215No Hit
ACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAA960.16973125884016974No Hit
GTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAAGAAAAGGCTTA960.16973125884016974No Hit
AGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATG930.16442715700141441No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG920.16265912305516267No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG910.1608910891089109No Hit
GCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTC890.15735502121640735No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA890.15735502121640735No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC860.15205091937765203No Hit
GTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAG850.15028288543140028No Hit
GCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAAC800.14144271570014144No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT800.14144271570014144No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA780.1379066478076379No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA780.1379066478076379No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG770.13613861386138612No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT760.13437057991513437No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA750.1326025459688826No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG750.1326025459688826No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG740.13083451202263083No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC730.12906647807637905No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA730.12906647807637905No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT720.1272984441301273No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC720.1272984441301273No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG710.12553041018387553No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA710.12553041018387553No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC700.12376237623762376No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC700.12376237623762376No Hit
GCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTT670.11845827439886847No Hit
GTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACCCAACAATTTA670.11845827439886847No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA670.11845827439886847No Hit
ATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGA660.1166902404526167No Hit
GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC650.11492220650636492No Hit
GAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGC650.11492220650636492No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC640.11315417256011315No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT640.11315417256011315No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT640.11315417256011315No Hit
GGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACC630.11138613861386137No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG610.10785007072135785No Hit
CGTACAAGGTATCTGAACACCTTTCTTAAATTGTTCATAAGAAAGTGTGC610.10785007072135785No Hit
TGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTA590.10431400282885431No Hit
CCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAAGAAAAGGCT580.10254596888260256No Hit
CTATAGTAGTCGGCATAGATGCTTTAATTCTAGAATTTGTACTTCTAGTA580.10254596888260256No Hit
GTGTATGCCCCTCCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACT580.10254596888260256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCTT151.223925E-4145.08
TTTTCGG151.223925E-4145.05
TTTTTCG151.223925E-4145.04
TGGAGCT100.007073308145.04
ATGGAGC100.007073308145.03
CGGCTTT151.223925E-4145.09
TTCGGCT151.223925E-4145.07
TTTCGGC151.223925E-4145.06
GAGCTGA352.7279202E-7103.571431
AATGGTG353.4092518E-582.857145
TATCCAG406.609017E-572.54
AGTTGCG406.609017E-572.59
CTATCCA406.609017E-572.53
TAAATTA554.0123086E-665.9090964
ATCCAGT451.1841807E-464.444445
TCTATCC451.1841807E-464.444442
AGCGTTA451.1841807E-464.444448
GTAGCGT451.1841807E-464.444446
AACAGTA851.2760211E-859.7058836
ACACCCC753.6976962E-758.0000045
Produced by FastQC (version 0.11.5)