FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153280
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT21451.3993997912317326No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA12080.7881002087682671No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT11590.7561325678496869No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT8130.530401878914405No Hit
GTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTG7850.512134655532359No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT7670.5003914405010438No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG7400.48277661795407095No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC6740.43971816283924847No Hit
GTATAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCC6190.40383611691022964No Hit
CTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTG5810.37904488517745305No Hit
GGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCA5410.3529488517745303No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT5290.34512004175365346No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC5240.3418580375782881No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC5180.33794363256784965No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA5140.3353340292275574No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA4950.32293841336116913No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC4860.31706680584551145No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT4840.3157620041753653No Hit
GCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCT4820.3144572025052192No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA4650.30336638830897705No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT4640.302713987473904No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG4600.3001043841336117No Hit
CTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATCAGTGCCAAGA4540.2961899791231733No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT4360.28444676409185804No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC4230.27596555323590816No Hit
GTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTC4180.2727035490605428No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG3870.2524791231732777No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG3850.2511743215031315No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC3790.24725991649269313No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT3700.24138830897703548No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG3670.23943110647181629No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA3570.2329070981210856No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG3340.217901878914405No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA3270.21333507306889352No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA3250.2120302713987474No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA3170.20681106471816282No Hit
CTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTT3160.20615866388308976No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT3140.20485386221294363No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC3080.20093945720250522No Hit
GTATAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC3060.1996346555323591No Hit
CTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATT2970.19376304801670144No Hit
CTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGC2960.19311064718162838No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT2910.18984864300626306No Hit
CTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCT2850.18593423799582465No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC2840.18528183716075156No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG2840.18528183716075156No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT2840.18528183716075156No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA2820.18397703549060543No Hit
GTGTTTAATTTATAGTTGCCAATCCTGTAGCGACTGTATGCAGCAAAACC2820.18397703549060543No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT2820.18397703549060543No Hit
GCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAAT2800.18267223382045927No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC2720.17745302713987474No Hit
CTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCG2720.17745302713987474No Hit
TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC2700.1761482254697286No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA2690.17549582463465555No Hit
GTTGTACCTCTAACACACTCTTGGTAGTGATAAAGCTCACAAGTAGCGAG2690.17549582463465555No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG2670.1741910229645094No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA2610.17027661795407098No Hit
GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTC2520.16440501043841338No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG2520.16440501043841338No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA2470.161143006263048No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA2440.15918580375782881No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA2390.15592379958246347No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT2390.15592379958246347No Hit
CTTTAAAAGTCCTCATAATAATTAGTAATATCTCTGCTATAGTAACCTGA2380.1552713987473904No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT2370.15461899791231734No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA2340.15266179540709812No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC2320.151356993736952No Hit
CTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTG2310.15070459290187893No Hit
CTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATT2300.15005219206680584No Hit
GTCCTAGATGGTGTCCAGCAATACGAAGATGTCCACGAAGGATCACAGCT2300.15005219206680584No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT2290.14939979123173278No Hit
GTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGC2280.1487473903966597No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT2260.14744258872651356No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG2210.1441805845511482No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC2170.14157098121085596No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA2160.1409185803757829No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTAC2130.13896137787056367No Hit
TTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATC2130.13896137787056367No Hit
GGTTTATGATGTAATCAAGATTCCAAATGGAAACTTTAAAAGTCCTCATA2110.13765657620041755No Hit
ATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTAC2090.1363517745302714No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC2080.13569937369519833No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC2080.13569937369519833No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT2050.1337421711899791No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC2040.13308977035490605No Hit
AGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGG2040.13308977035490605No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT2030.13243736951983298No Hit
CATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCA2020.13178496868475992No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG2020.13178496868475992No Hit
TTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAG2010.13113256784968685No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC2000.1304801670146138No Hit
GTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTAGGTTCCT2000.1304801670146138No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC1960.1278705636743215No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT1950.12721816283924844No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT1930.1259133611691023No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT1930.1259133611691023No Hit
CCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCG1910.12460855949895616No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC1890.12330375782881No Hit
CTCTAACACACTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATC1890.12330375782881No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT1870.12199895615866388No Hit
GCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTC1840.12004175365344467No Hit
GGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCG1820.11873695198329855No Hit
GGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGC1790.11677974947807933No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA1770.1154749478079332No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC1770.1154749478079332No Hit
GATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCT1770.1154749478079332No Hit
GTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGG1730.11286534446764093No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA1720.11221294363256784No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC1710.11156054279749478No Hit
TCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTC1710.11156054279749478No Hit
GAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTG1700.11090814196242171No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC1680.10960334029227556No Hit
ATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAG1680.10960334029227556No Hit
ATTATACAGTTTCCTGTTCACCTTTTACAATTAATTGCCAGGAACCTAAA1670.1089509394572025No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG1640.10699373695198329No Hit
GTATCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA1620.10568893528183716No Hit
ATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACACGCTG1610.1050365344467641No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA1610.1050365344467641No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC1600.10438413361169101No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA1600.10438413361169101No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG1600.10438413361169101No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1600.10438413361169101No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC1600.10438413361169101No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1590.10373173277661794No Hit
ACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAG1580.10307933194154488No Hit
CTCCTACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCA1580.10307933194154488No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG1570.10242693110647182No Hit
CCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTC1560.10177453027139875No Hit
GTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT1560.10177453027139875No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG1550.10112212943632568No Hit
GTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCG1550.10112212943632568No Hit
GATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCAT1550.10112212943632568No Hit
CTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGA1540.10046972860125261No Hit
GTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACT1540.10046972860125261No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTCACCTTTTACAATTA1540.10046972860125261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAG151.2279896E-4145.01
TAACCTC151.2279896E-4145.05
ATCCTCC100.007085047144.999986
GTTCAGG100.007085047144.999981
GATCCGT100.007085047144.999987
ACTTAAC100.007085047144.999982
TCCGTTC100.007085047144.999989
ATCCGTT100.007085047144.999988
AGATCCG100.007085047144.999986
GTCAGAC202.1247943E-6144.999981
TAGATCC100.007085047144.999985
TATAACC950.0114.473682
CTTGTAT203.8597456E-4108.749991
CTTAACC203.8597456E-4108.749993
GTTCCTA553.6379788E-12105.4545441
ATAACCT1050.0103.571433
GTATAAC1150.0100.869571
GGCTTGT1500.091.8333361
TCTAATC259.371479E-487.09
GAGTAAG259.371479E-487.01
Produced by FastQC (version 0.11.5)