FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178877
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATT22961.2835635660258164No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC17460.9760897152792143No Hit
GTTTAATTGTGTACAAAAACTGCCATATTGCAACAAAAGATTGCTGCATT8290.46344694957987886No Hit
GTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGA8090.4522660822800025No Hit
CAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACAC7740.4326995645052186No Hit
GCATAATTGTCACCATTACTATGGCAATCAAGCCAGCTATAAAACCTAGC7410.4142511334604225No Hit
GTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATT7330.40977878654047195No Hit
GTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCC7030.39300748559065724No Hit
ATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGC6410.35834679696104027No Hit
GAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATAC6170.34492975620118854No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACG5820.32536323842640474No Hit
GGTAATATTTGTGAAAAATTAAAACCACCAAAATCTTTAATTGGTGGTGT5670.3169775879514974No Hit
CTATAAAACCTAGCCAAATGTACCATGGCCATTTTATATACTGCTCATAC5360.2996472436366889No Hit
CTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATT5260.29405680998675066No Hit
GTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGG5240.29293872325676307No Hit
GGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGA5170.28902541970180623No Hit
GCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAA5170.28902541970180623No Hit
TCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAA5010.2800807258619051No Hit
TGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATA4840.27057698865701013No Hit
GTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAA4630.25883707799213984No Hit
CTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTC4610.2577189912621522No Hit
GGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGAC4600.25715994789715835No Hit
ACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGAAAGGATA4560.25492377443718306No Hit
GTGCAGAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAA4510.252128557612214No Hit
ATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATA4450.24877429742225107No Hit
ACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCT4440.2482152540572572No Hit
GCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAA4430.24765621069226337No Hit
GTTACCAGACACAAATGTGTTGTCTGTAGTAATGATTTGTGGTTCATAAA4220.2359163000273931No Hit
TTACTATGGCAATCAAGCCAGCTATAAAACCTAGCCAAATGTACCATGGC4210.2353572566623993No Hit
GTTTTGATGGATCTGGTAATATTTGTGAAAAATTAAAACCACCAAAATCT4210.2353572566623993No Hit
GCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTAT4190.23423916993241164No Hit
ATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAA4180.23368012656741782No Hit
GGACATAAGATGATAGCCCTTTCCACAAAAATCAACTCTTTTTGATTGTC4160.23256203983743018No Hit
GTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGA4110.2297668230124611No Hit
AGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTT4080.22808969291747963No Hit
GTTTACAACTGAAGCATTAATGCCAGAGATGTCACCTAAATCAACATCTG4030.2252944760925105No Hit
ACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAAC3880.2169088256176032No Hit
CCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAAAAG3860.2157907388876155No Hit
GCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTC3840.21467265215762785No Hit
ATCTGAAGGAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTACAGTTC3810.21299552206264638No Hit
TAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAG3780.21131839196766494No Hit
ATTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGAT3780.21131839196766494No Hit
GTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTC3740.20908221850768965No Hit
GAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAA3740.20908221850768965No Hit
CATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGAT3730.20852317514269583No Hit
CTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCA3660.20460987158773905No Hit
TTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACT3590.2006965680327823No Hit
CCTTTGAGCACTGGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCC3580.20013752466778847No Hit
CTACAGCAACTGGTCATACAGCAAAGCATAATTGTCACCATTACTATGGC3540.19790135120781321No Hit
AGTACACACTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCTCTGAT3520.19678326447782557No Hit
GACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTG3470.19398804765285643No Hit
CCATTACTATGGCAATCAAGCCAGCTATAAAACCTAGCCAAATGTACCAT3430.19175187419288114No Hit
AGACTACACCATGAGGTGCTGACTGAGGGAAGGACATAAGATGATAGCCC3310.18504335381295528No Hit
CTCCAATACCATTAAACCTATAAGCCATTTGCATAGCAAATGGTATTTGT3190.17833483343302942No Hit
TTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAAT3170.17721674670304177No Hit
GTCCAAGTACACACTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCT3160.17665770333804792No Hit
ACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAAT3150.1760986599730541No Hit
CTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACA3110.1738624865130788No Hit
TCACCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAA3100.173303443148085No Hit
CTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCA3080.17218535641809737No Hit
AGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAAACCAACCAACTT3030.16939013959312824No Hit
GTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTGACAATTCCTA3010.1682720528631406No Hit
TTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAAC3000.16771300949814677No Hit
CTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGC2990.16715396613315295No Hit
GAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTACAGTTCCAATTGTG2970.1660358794031653No Hit
TTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATA2960.16547683603817148No Hit
GCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTCATTTAAATTC2940.16435874930818384No Hit
GAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAAACAGT2940.16435874930818384No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA2930.16379970594319002No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2890.16156353248321473No Hit
GTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATG2880.16100448911822088No Hit
TTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGA2800.15653214219827033No Hit
ATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTT2780.15541405546828266No Hit
GACATAAGATGATAGCCCTTTCCACAAAAATCAACTCTTTTTGATTGTCC2760.15429596873829501No Hit
GTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAA2710.15150075191332593No Hit
GTGATCAACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGA2630.14702840499337533No Hit
GTTTAAAGCTTGTGCATTTTGGTTGACCACATCTTGAAGTTTTCCAAGTG2620.14646936162838153No Hit
CAACTACAGCAACTGGTCATACAGCAAAGCATAATTGTCACCATTACTAT2590.14479223153340004No Hit
GGATCATAAACTGTGTTGTTGACAATTCCTATTACAACATCACAGTTACC2580.14423318816840622No Hit
CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG2580.14423318816840622No Hit
CTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCA2570.1436741448034124No Hit
GGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAA2560.14311510143841857No Hit
ATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTG2550.14255605807342475No Hit
GACTAATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG2530.1414379713434371No Hit
CTCTAGCAGCAATATCACCAAGGCAATCACCATATTGTTTGATGAAGCCA2530.1414379713434371No Hit
GCTATAAAACCTAGCCAAATGTACCATGGCCATTTTATATACTGCTCATA2520.14087892797844329No Hit
CTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTCATTTAAAT2510.14031988461344946No Hit
CAAGAAGACTACACCATGAGGTGCTGACTGAGGGAAGGACATAAGATGAT2460.13752466778848035No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAA2440.1364065810584927No Hit
GTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATT2400.13417040759851742No Hit
GTATTGAGCAATCATTTCATCTGTGAGCAAAGGTGGCAAAACAGTAAGGC2400.13417040759851742No Hit
AACAAAATCTGAAGGAGTAGCATCCTTGATTTCACCTTGCTTCAAAGTTA2360.13193423413854213No Hit
GATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCAC2330.13025710404356067No Hit
GTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAAT2330.13025710404356067No Hit
TTTGAGCACTGGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCCAC2290.12802093058358535No Hit
ATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTG2280.12746188721859153No Hit
GTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTG2270.12690284385359774No Hit
CATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGC2260.1263438004886039No Hit
TCATTGAGGCGGTCAATTTCTTTTTGAATGTTTACAACTGAAGCATTAAT2220.1241076270286286No Hit
GTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAAC2220.1241076270286286No Hit
TCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATG2220.1241076270286286No Hit
GTAGTAATGATTTGTGGTTCATAAAAATTCCTTTGTGTTACAAACCAGTG2190.12243049693364715No Hit
CTTTTTGATTGTCCAAGTACACACTCTGACATTTTAGTAGCAGCAAGATT2170.1213124102036595No Hit
GGCTCAGAGTCGTCTTCATCAAATTTGCAGCAGGATCCACAAGAACAACA2160.12075336683866568No Hit
ATCATAAACTGTGTTGTTGACAATTCCTATTACAACATCACAGTTACCAG2130.11907623674368421No Hit
CTATTAAATTGGTTGGCAATCAATTTTTGGTTCTCATAGAGAACATTCTG2130.11907623674368421No Hit
GTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATC2100.11739910664870273No Hit
GTGTAACTCCAATACCATTAAACCTATAAGCCATTTGCATAGCAAATGGT2090.11684006328370891No Hit
ACATATGTCTGCAAACTTTGAAGTCTGCCTGTGATCAACCTATCAATTTG2070.11572197655372127No Hit
GAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATG2050.11460388982373364No Hit
CTCTGACATTTTAGTAGCAGCAAGATTAGCAGAAGCTCTGATTTCTGCAG2050.11460388982373364No Hit
GGTCATACAGCAAAGCATAATTGTCACCATTACTATGGCAATCAAGCCAG2050.11460388982373364No Hit
GTTCAACAGCTATTCCAGTTAAAGCACGGTTTAATTGTGTACAAAAACTG2050.11460388982373364No Hit
GTTGTGAAGTTCTTTTCTTGTGCAGGGACATAAGTCACATGCAAGAAGAC2040.11404484645873982No Hit
GCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGAC2040.11404484645873982No Hit
TCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATA2030.11348580309374597No Hit
ATAAATGACCTCTTGCTTGGTTTTGATGGATCTGGTAATATTTGTGAAAA2030.11348580309374597No Hit
GTTGAAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGAT2030.11348580309374597No Hit
GACATAAGTCACATGCAAGAAGACTACACCATGAGGTGCTGACTGAGGGA2010.11236771636375834No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG2000.11180867299876451No Hit
AGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAG1990.11124962963377069No Hit
TCCTTGATTTCACCTTGCTTCAAAGTTACAGTTCCAATTGTGAAGATTCT1980.11069058626877687No Hit
GAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTG1960.10957249953878923No Hit
CATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACA1960.10957249953878923No Hit
CCTATAAGCCATTTGCATAGCAAATGGTATTTGTAATGCAGCACCTGCAC1910.1067772827138201No Hit
CTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGC1900.1062182393488263No Hit
GAGTCGTCTTCATCAAATTTGCAGCAGGATCCACAAGAACAACAGCCCTT1860.103982065888851No Hit
ACCATATTGTTTGATGAAGCCAGCATCTGCAAGTGTCACTTTGTTGAAAA1860.103982065888851No Hit
GTATATGCGCTAGTTATCAGACTCAGACTAATACCTTCCCAGGTAACAAA1840.10286397915886335No Hit
TTGTAATGCAGCACCTGCACCAAAGGTCCAACCAGAAGTGATTGTACCCG1830.10230493579386953No Hit
CAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACA1830.10230493579386953No Hit
ATTTAAAACACTTGAAATTGCACCAAAATTGGAGCTAAGTTGTTTAACAA1810.10118684906388188No Hit
CATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAG1800.10062780569888806No Hit
GTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCAT1790.10006876233389424No Hit
GTCATACAGCAAAGCATAATTGTCACCATTACTATGGCAATCAAGCCAGC1790.10006876233389424No Hit
GATTAGCAGAAGCTCTGATTTCTGCAGCTCTAATTAATTGTTGAGTCACA1790.10006876233389424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCC100.0070860283145.000028
CGTTAGT100.0070860283145.000029
TGCGCCA100.0070860283145.000026
GTCAATG100.0070860283145.000029
TCGGCTT202.1257747E-6145.000028
TTTTCGG202.1257747E-6145.000025
TGTTCGA100.0070860283145.000027
ATTGGCG100.0070860283145.000026
TTTTTCG202.1257747E-6145.000024
CGCCAGT100.0070860283145.000028
ATGCGTT100.0070860283145.000026
ATGCGCC100.0070860283145.000025
GCCAGGT100.0070860283145.000028
GCGCCAG100.0070860283145.000027
GCGTTAG100.0070860283145.000028
CGGCTTT202.1257747E-6145.000029
TTGGCGG100.0070860283145.000027
TGGCGGT100.0070860283145.000028
ACTAGCC100.0070860283145.000022
TTAATTC100.0070860283145.000023
Produced by FastQC (version 0.11.5)