FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161195
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATG10460.6489035019696641No Hit
GCTAGACACCTAGTCATGATTGCATCACAACTAGCTACATGTGCATTACC9050.5614318061974627No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT8780.5446819070070411No Hit
GAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTG8250.5118024752628804No Hit
GTCATATTCTGAGCCCTGTGATGAATCAACAGTTTGAGTTGGTAGTCCCA7930.49195074288904744No Hit
GTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC7300.45286764477806385No Hit
ACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTACCTGTAA6210.3852476813796954No Hit
CTCGTGGACAGCTAGACACCTAGTCATGATTGCATCACAACTAGCTACAT5920.3672570489159093No Hit
TGACAATACAGATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTG5180.32134991780142064No Hit
GTACATAAGTGGTATGAGGTGTTTAAATTGATCTCCAGGCGGTGGTTTAG5040.3126647848878687No Hit
TTACAAGAGTGAGCTGTTTCAGTGGTTTGAGTGAATATGACATAGTCATA5030.31204441825118645No Hit
CCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAAT4620.286609386147213No Hit
CTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAG4610.28598901951053074No Hit
GCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGA4290.2661372871366978No Hit
ATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAAT4230.2624150873166041No Hit
GTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTA4070.25248922112968764No Hit
GTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTTCAGTTTTGAATT4020.24938738794627624No Hit
GTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTC4020.24938738794627624No Hit
GGTAATAGCAACATTAAATCTGTTTACATTACAAGAGTGAGCTGTTTCAG3990.24752628803622942No Hit
ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA3880.24070225503272433No Hit
CATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTAT3810.23635968857594838No Hit
CTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAAT3740.23201712211917244No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT3730.23139675548249014No Hit
GTGTAGGATGTAACCCAGTGATTACCTTACTACAATCTTTAAAAAGTCCT3630.22519308911566738No Hit
CTATCAGACATTATGCAAAGTATGCCTACTTTTGCTCTGGTAATAGCAAC3600.2233319892056205No Hit
GATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATG3570.22147088929557368No Hit
GTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCAT3540.21960978938552686No Hit
GACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT3530.21898942274884456No Hit
CCATTAACTTGATAATTCATTTTAAAACCCATCATAGAGATGAGTCTTCT3520.21836905611216229No Hit
ATCATAAACGGATTATAGACGTAATCAAATCCAATAGAATGATGCCAACA3460.21464685629206862No Hit
CATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTA3450.21402648965538634No Hit
GAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTG3380.20968392319861034No Hit
ATTCTGAGCCCTGTGATGAATCAACAGTTTGAGTTGGTAGTCCCAAAATC3220.1997580570116939No Hit
GTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAAT3220.1997580570116939No Hit
ATTTTGTGCTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC3180.19727659046496476No Hit
GTCTTATAGCTTCTTCGCGGGTGATAAACATGTTAGGGTAACCATTAACT3180.19727659046496476No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA3180.19727659046496476No Hit
GTATTATTAGGTGTATCAACATAACCTGTAGGTACAGCAACTAGGTTAAC3180.19727659046496476No Hit
CTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACA3150.19541549055491794No Hit
CCTTACTACAATCTTTAAAAAGTCCTGTTACATTTTCAGCTTGTAAAGTT3000.18610999100468378No Hit
TCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTT2980.1848692577313192No Hit
GGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGC2930.1817674245479078No Hit
ATACAGATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTGTTGAA2900.179906324637861No Hit
GTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAG2890.1792859580011787No Hit
GATTATAGACGTAATCAAATCCAATAGAATGATGCCAACAGGCATAAGTG2870.17804522472781414No Hit
AGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGAC2820.17494339154440272No Hit
GATCATGGTTGCTTTGTAGGTTACCTGTAAAACCCCATTGTTGAACATCA2800.1737026582710382No Hit
ACACTGAGGTGTGTAGGTGCCTGTGTAGGATGTAACCCAGTGATTACCTT2790.1730822916343559No Hit
ATCTTTATACGCACTACATTCCAAGGAAGTCCTTTGTACATAAGTGGTAT2760.17122119172430908No Hit
GTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCAT2670.16563789199416856No Hit
AGTCATGATTGCATCACAACTAGCTACATGTGCATTACCATGGACTTGAC2650.164397158720804No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC2630.16315642544743944No Hit
GGTATGAGGTGTTTAAATTGATCTCCAGGCGGTGGTTTAGCACTAACTCT2580.16005459226402804No Hit
GTTCTAATGTTGAATTCACTTTGAATTTATCAAAACACTCTACACGAGCA2570.15943422562734577No Hit
CCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATAC2550.1581934923539812No Hit
TAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATC2540.15757312571729892No Hit
CAGCTAGACACCTAGTCATGATTGCATCACAACTAGCTACATGTGCATTA2540.15757312571729892No Hit
CTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGC2510.15571202580725207No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT2470.15323055926052298No Hit
CTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGC2430.15074909271379386No Hit
GTCTTCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAA2420.15012872607711156No Hit
CATTAAATCTGTTTACATTACAAGAGTGAGCTGTTTCAGTGGTTTGAGTG2410.14950835944042928No Hit
CTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCT2380.14764725953038246No Hit
AGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTT2370.1470268928937002No Hit
GTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTC2370.1470268928937002No Hit
CATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACA2350.14578615962033561No Hit
GTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTC2350.14578615962033561No Hit
GTTGAACATCAATCATAAACGGATTATAGACGTAATCAAATCCAATAGAA2350.14578615962033561No Hit
GTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGT2350.14578615962033561No Hit
AGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAG2340.14516579298365334No Hit
TACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATAT2330.14454542634697107No Hit
GTCTTTATGTGCTTTAAGCTTATTATCATAAACCAAAGCACTCACAGTGT2320.14392505971028877No Hit
CTTTGTAGGTTACCTGTAAAACCCCATTGTTGAACATCAATCATAAACGG2290.14206395980024195No Hit
CTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCAT2290.14206395980024195No Hit
TTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCC2290.14206395980024195No Hit
CCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCAT2280.14144359316355967No Hit
GAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGG2270.1408232265268774No Hit
CCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAA2260.1402028598901951No Hit
CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC2220.13772139334346598No Hit
ATCAGACATTATGCAAAGTATGCCTACTTTTGCTCTGGTAATAGCAACAT2210.1371010267067837No Hit
CTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGC2190.13586029343341915No Hit
GATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGA2170.1346195601600546No Hit
TCTTATAGCTTCTTCGCGGGTGATAAACATGTTAGGGTAACCATTAACTT2150.13337882688669003No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC2140.13275846025000776No Hit
GAATATGACATAGTCATATTCTGAGCCCTGTGATGAATCAACAGTTTGAG2140.13275846025000776No Hit
GCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATA2120.13151772697664318No Hit
TTTTAAAACCCATCATAGAGATGAGTCTTCTATAGGTCATGTCCTTAGGT2120.13151772697664318No Hit
TTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCAC2110.1308973603399609No Hit
ACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTA2100.13027699370327864No Hit
GTCATAAAGGTCTCTATCAGACATTATGCAAAGTATGCCTACTTTTGCTC2090.12965662706659636No Hit
GTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGC2090.12965662706659636No Hit
CAACTAGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTT2060.12779552715654952No Hit
ATTATTAGGTGTATCAACATAACCTGTAGGTACAGCAACTAGGTTAACAC2060.12779552715654952No Hit
TCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATG2060.12779552715654952No Hit
CTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAA2050.12717516051986724No Hit
TTCTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTAT2030.12593442724650267No Hit
GTTCTAGTGTGCCCTTAGTTAGCAATGTGCGTGGTGCAGGTAATTGAGCA2010.12469369397313812No Hit
GCACTCGTGGACAGCTAGACACCTAGTCATGATTGCATCACAACTAGCTA2010.12469369397313812No Hit
CTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTG2010.12469369397313812No Hit
GTTAATTGCAGATGAAACATCATGCGTGATAACACCCTTATAAAACATTT2000.12407332733645583No Hit
ATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGA2000.12407332733645583No Hit
GGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCT2000.12407332733645583No Hit
TTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAG1990.12345296069977357No Hit
GTACAAAAGACATACTGTTCTAATGTTGAATTCACTTTGAATTTATCAAA1980.1228325940630913No Hit
GTGTAGGTGCCTGTGTAGGATGTAACCCAGTGATTACCTTACTACAATCT1960.12159186078972674No Hit
TGTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCA1960.12159186078972674No Hit
TCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAAC1950.12097149415304445No Hit
ATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTA1950.12097149415304445No Hit
GCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTA1940.12035112751636216No Hit
TCATAGAGATGAGTCTTCTATAGGTCATGTCCTTAGGTATGCCAGGTATG1940.12035112751636216No Hit
TTATAGACGTAATCAAATCCAATAGAATGATGCCAACAGGCATAAGTGTC1930.11973076087967989No Hit
GTGATTACCTTACTACAATCTTTAAAAAGTCCTGTTACATTTTCAGCTTG1920.1191103942429976No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA1920.1191103942429976No Hit
CCTGTAGGTACAGCAACTAGGTTAACACCTGTAGAAAAACCTAGCTGTAA1920.1191103942429976No Hit
CTCTAGTAGCATGACACCCCTCGACATCGAAGCCAATCCATGCACGTACA1910.11849002760631533No Hit
CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA1900.11786966096963304No Hit
CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAG1890.11724929433295078No Hit
GTGCAGGTAATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGT1880.11662892769626851No Hit
ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTA1870.11600856105958622No Hit
GCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAA1850.11476782778622166No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1830.1135270945128571No Hit
GTCTATCACATAGACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATAC1830.1135270945128571No Hit
ATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAAT1820.11290672787617481No Hit
ACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGT1820.11290672787617481No Hit
TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG1810.11228636123949254No Hit
TGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCT1770.10980489469276343No Hit
AGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGG1770.10980489469276343No Hit
GTAAAACCCCATTGTTGAACATCAATCATAAACGGATTATAGACGTAATC1770.10980489469276343No Hit
GCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCAC1760.10918452805608114No Hit
AATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGA1760.10918452805608114No Hit
ATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACT1750.10856416141939887No Hit
ACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAA1730.10732342814603431No Hit
CTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGT1730.10732342814603431No Hit
GTCTGAAGCAGTGGAAAAGCATGTGGCACGTCTATCACATAGACAACAGG1730.10732342814603431No Hit
CCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATG1730.10732342814603431No Hit
GCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCA1720.10670306150935202No Hit
CTGTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCA1720.10670306150935202No Hit
GACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATACTTCATAGATGTC1710.10608269487266975No Hit
GTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCATAATTTGTGG1710.10608269487266975No Hit
ATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCATCATAGA1710.10608269487266975No Hit
GCTTTAAGCTTATTATCATAAACCAAAGCACTCACAGTGTCAACAATTTC1700.10546232823598746No Hit
AATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCT1670.10360122832594064No Hit
CCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGA1660.10298086168925835No Hit
ACTCAAAGCCATGTGCCCATAAGACAAATACGACTCTGTCAGAGAGATTT1650.10236049505257608No Hit
GGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAA1650.10236049505257608No Hit
GTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGCGAGCAGAA1640.10174012841589379No Hit
CTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTTC1620.10049939514252923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC100.007085384144.999986
TTAACGT100.007085384144.999984
TTTGTGC1250.098.63
TGCTGAC1350.091.296297
GCTGACA1400.088.035718
ACTGAAC259.372364E-487.03
CTGAACA259.372364E-487.04
TGAACAA259.372364E-487.05
GTGCTGA1450.085.06
TTGTGCT1550.079.516134
TCGTGGA1400.077.678572
CTCGTGG1400.077.678571
TGTGCTG1650.074.6969765
CTGACAT1700.072.59
CGTGGAC1150.063.0434723
AGTAATG1900.061.0526352
GCTAGAC2600.058.557691
AAATTAG502.0051654E-458.05
GCACTCG502.0051654E-458.01
CTAGACA2650.057.4528272
Produced by FastQC (version 0.11.5)