FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205971
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG11010.5345412703730137No Hit
TCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGA8400.4078244024644246No Hit
GTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACC8210.3985998028848721No Hit
ATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATT6940.3369406372741794No Hit
TTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG6890.33451311106903403No Hit
AATTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT6710.32577401673051065No Hit
GTAGTAAACAGACGCTGATTTTGCAGATGATTCTTCACATTTTGATTTAC6530.3170349223919872No Hit
GTTTAGACATGACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACA6520.31654941715095813No Hit
GTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAGTTGCAACTAGTG6060.2942161760636206No Hit
TAATAAAATGATGCAAAGAAGATGTACATTCTAACCATAGCTGAAATCGG5910.2869335974481844No Hit
CTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGT5800.2815930397968646No Hit
GTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGC5640.2738249559403994No Hit
CTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATT5520.26799889304805047No Hit
GTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATC5500.2670278825659923No Hit
CATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAG5370.2607163144326143No Hit
GTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGT5250.2548902515402654No Hit
CTGTAACACTATCAACGATGTAAGAAGACTGGTCAGTAGGATTTATTGGT5230.25391924105820723No Hit
GTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCA5010.24323812575556755No Hit
TCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTT4970.24129610479145122No Hit
GTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACA4920.23886857858630584No Hit
CAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGCTGATTTTG4770.23158599997086968No Hit
GTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAACTGTAGTGACA4690.22770195804263704No Hit
GTATAGAACCAGTACAGTAGGTTGCAATAGTGACATTAGTAGAGTTCAAA4670.2267309475605789No Hit
GTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAA4630.2247889265964626No Hit
GGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACAC4610.22381791611440446No Hit
GTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAA4570.22187589515028816No Hit
CCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAG4510.21896286370411366No Hit
CTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGC4500.2184773584630846No Hit
TTCTAACACCATTAACAATAGTTGTACATTCGACTCTTGTTGCTCTATTA4350.21119477984764845No Hit
CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG4330.21022376936559029No Hit
GACATGAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACA4190.2034266959911832No Hit
GTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGC4190.2034266959911832No Hit
CAATTAACACAATTCCAATTGTGTAGTTTGCAAAAGCCTTTACCTCCATT4160.201970180268096No Hit
CTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTG4130.20051366454500877No Hit
ATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTG4090.19857164358089247No Hit
GTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTA4090.19857164358089247No Hit
CAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGAC4040.19614411737574705No Hit
GCATATAGTTATTACAACTATCGCCAGTAACTTCTATGTCAGATTGATGT3940.1912890649654563No Hit
ACCATCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTT3840.18643401255516553No Hit
ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGT3760.18254997062693293No Hit
GCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGACATGAC3680.1786659286987003No Hit
CTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGG3650.17720941297561307No Hit
CTAATAAATGTACTACCAGCACAGAATGTATCACAATTAACACAATTCCA3640.176723907734584No Hit
ATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAAC3600.1747818867704677No Hit
GGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTA3600.1747818867704677No Hit
TTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTA3540.17186885532429322No Hit
ACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTT3520.17089784484223508No Hit
CTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACA3510.170412339601206No Hit
GTACTAATTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTG3490.16944132911914783No Hit
TTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTT3460.1679848133960606No Hit
AGATTAATTATTAACCACATAAGCCAAGAATTACTAATAAAATGTACTGC3420.1660427924319443No Hit
GTCTAGTAGTTGCACATGTCAACTTAAAAGGTAAGTTATTCTTTTTAGCA3410.16555728719091523No Hit
CCCTTAAGTGCTATCTTTGTTGTTACAACATTAACAACTTGTCTAGTAGT3360.16312976098576984No Hit
GCACAGAATGTATCACAATTAACACAATTCCAATTGTGTAGTTTGCAAAA3360.16312976098576984No Hit
CCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGC3300.16021672953959537No Hit
GGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTT3200.15536167712930463No Hit
GATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAAC3160.1534196561651883No Hit
GTCTAAGTTAACAAAATGAGAGAGAGAATGTCTTTCATAAGTCTTTTGAC3100.15050662471901383No Hit
TCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAGA3080.1495356142369557No Hit
ATCAATAGCCTTGTATCCTATGATTTCACTTGAAAAGTCAGTATGTTTAG3060.14856460375489755No Hit
GTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATGT3030.1471080880318103No Hit
ATTTAAGACATTCAACAACATCTTTAGTTTCTACATCTGAATCAACAAAC2980.14468056182666492No Hit
CCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGA2950.1432240461035777No Hit
AGCCAAGAATTACTAATAAAATGTACTGCAAAATAGCTGAAAAACAATTG2910.1412820251394614No Hit
CTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATC2870.13934000417534506No Hit
GTATTAACGTAAGCATCAAACATTTTAACTGCAACTTCCGCACTATCACC2810.1364269727291706No Hit
CTTCTATGTCAGATTGATGTGACAATTTAAGACATTCAACAACATCTTTA2780.13497045700608337No Hit
CATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGAT2780.13497045700608337No Hit
GCTTTATCAAAGTAAAGATGGATGGAACCATTCTTCACTGTAACACTATC2760.13399944652402523No Hit
GTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATG2760.13399944652402523No Hit
TTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAAT2760.13399944652402523No Hit
GTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATA2740.13302843604196707No Hit
CCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGT2700.13108641507785077No Hit
GTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAGAGAATGTC2660.12914439411373446No Hit
AATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACA2660.12914439411373446No Hit
TCATACAAGTTGATGAATTACAACCGTCTACAACATGCACATAACTTTTC2610.12671686790858908No Hit
ACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA2610.12671686790858908No Hit
GTAGTGACAAGTCTCTCGCAACTTCATCACTAATAAATGTACTACCAGCA2590.12574585742653094No Hit
GAATGTATCACAATTAACACAATTCCAATTGTGTAGTTTGCAAAAGCCTT2580.12526035218550183No Hit
GCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGG2580.12526035218550183No Hit
TTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTA2560.12428934170344369No Hit
CACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAA2550.12380383646241461No Hit
ATTCTTCACTGTAACACTATCAACGATGTAAGAAGACTGGTCAGTAGGAT2520.12234732073932739No Hit
ATTGCAGCCAATCCAAGTACATAGAAAAACCTAGTGAAAAGAATATATGC2520.12234732073932739No Hit
ATCACCAACATCAGACACTAATGCCTGATCTAGTAACAGTATAGGTTGAC2510.12186181549829832No Hit
GTCTACAACATGCACATAACTTTTCCATACATAATAAAATGATGCAAAGA2500.12137631025726922No Hit
GTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAAT2490.12089080501624014No Hit
AACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGT2490.12089080501624014No Hit
ATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAA2460.11943428929315292No Hit
CACATAAGCTGACTGTAGTAAACAGACGCTGATTTTGCAGATGATTCTTC2460.11943428929315292No Hit
ACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTT2450.11894878405212385No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2420.1174922683290366No Hit
GTACAGTAGGTTGCAATAGTGACATTAGTAGAGTTCAAATAGCCTTCTCT2410.11700676308800753No Hit
ATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATC2400.11652125784697845No Hit
TGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTAC2390.11603575260594938No Hit
GTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGT2390.11603575260594938No Hit
CTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAG2390.11603575260594938No Hit
TTTGTATAGTTTCTAAAGAAGGATAGGTGTCTAAAGAATCTAAACCACTA2390.11603575260594938No Hit
GAACAGGTGTTATTAAATAGAAAATAGCAGCAACAAAAAGGAACACAAGT2370.11506474212389123No Hit
CTATTACGTTTGTAACACATCATACAAGTTGATGAATTACAACCGTCTAC2360.11457923688286216No Hit
ATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTT2330.11312272115977491No Hit
ATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGAC2320.11263721591874584No Hit
AGATAAGACATTGTCTAAGGACACATTCTTTGCAAGTTCAGCTTCTGCAG2280.11069519495462954No Hit
TCTAGTAACAGTATAGGTTGACACATAAGCTGACTGTAGTAAACAGACGC2260.10972418447257137No Hit
CCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTAC2240.10875317399051322No Hit
CCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATT2240.10875317399051322No Hit
CGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAA2230.10826766874948415No Hit
GTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACT2220.10778216350845508No Hit
GTACAAGATTAATTATTAACCACATAAGCCAAGAATTACTAATAAAATGT2220.10778216350845508No Hit
CTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCT2200.10681115302639693No Hit
ATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTC2190.10632564778536784No Hit
CCATTAGCATAGACATAAAAGGACCTTCTAACACCATTAACAATAGTTGT2170.10535463730330968No Hit
GTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAAT2160.10486913206228062No Hit
GTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGAC2150.10438362682125153No Hit
GAAGATGTACATTCTAACCATAGCTGAAATCGGGGCCATTTGTACAAGAT2140.10389812158022246No Hit
GGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTT2120.10292711109816431No Hit
CTAATGCCTGATCTAGTAACAGTATAGGTTGACACATAAGCTGACTGTAG2120.10292711109816431No Hit
GTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCA2120.10292711109816431No Hit
TTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTGTCTAAGTT2100.10195610061610615No Hit
GTGTTATTAGCTCTCAGGTTGTCTAAGTTAACAAAATGAGAGAGAGAATG2090.10147059537507708No Hit
ACGTATTTGTTTTCGTAGTTGTTCAGACAATGACATGAAATCTTTAACGT2090.10147059537507708No Hit
TAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAACTGTAGTGACAA2090.10147059537507708No Hit
GTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGA2090.10147059537507708No Hit
TGTTATAGGTGAGCATATAGTTATTACAACTATCGCCAGTAACTTCTATG2080.100985090134048No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2080.100985090134048No Hit
GTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGA2070.10049958489301893No Hit
CTTTTAAACTGTAGTGACAAGTCTCTCGCAACTTCATCACTAATAAATGT2070.10049958489301893No Hit
CTATCAACGATGTAAGAAGACTGGTCAGTAGGATTTATTGGTCTTTTAAA2060.10001407965198984No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTT100.0070868046144.999989
AAATCAC100.0070868046144.999987
GTACCGG100.0070868046144.999987
AGGTGTG100.0070868046144.999986
TACCGGT100.0070868046144.999988
TGTACCG100.0070868046144.999986
GTGTGCA100.0070868046144.999988
GGTGTGC100.0070868046144.999987
AAGGACT203.8616522E-4108.749995
GCCTTAT900.088.6111157
ATAGGCG259.37609E-487.05
AGGCGTG259.37609E-487.07
GGATACA259.37609E-487.0145
ATTCAAT259.37609E-487.06
TAGGCGT259.37609E-487.06
TGCCTTA950.083.947376
TGTAGGC353.4307865E-582.857147
AATGTAG353.4307865E-582.857145
TAGGCCA353.4307865E-582.857149
CCTTATT1100.072.500018
Produced by FastQC (version 0.11.5)