FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287052
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA34481.2011760935300921No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG18210.6343798336189959No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT15100.5260370943243733No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC14680.5114055989855496No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC13830.48179423937126375No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT12950.45113777294706187No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC12130.4225715201426919No Hit
ATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAG11640.4055014422473977No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG11620.4048047043741203No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA10210.3556846843080696No Hit
CCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCT9910.3452336162089099No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT9700.33791786853949807No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA9290.3236347421373131No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC9100.3170157323411786No Hit
ATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAG8950.31179019829159876No Hit
CAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTT8880.30935161573512815No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA8750.30482281955882556No Hit
ACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAAC7980.2779984114376489No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG7370.2567479063026908No Hit
CATCAGCTCCATCCAAATAAGTTGGACCAAACTGTTGTCCATATGTCATT7310.25465769268285887No Hit
GGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAG7280.2536125858729429No Hit
CCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTTAAGATTGTCA7150.2490837896966403No Hit
CTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTA7130.24838705182336301No Hit
GCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTG7070.24629683820353107No Hit
TATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGG7030.24490336245697641No Hit
ATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATA6800.23689087691428728No Hit
ATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCT6730.2344522943578167No Hit
GAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAA6640.23131697392806877No Hit
GTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCA6560.2285300224349595No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG6550.22818165349832087No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC6460.22504633306857294No Hit
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTA6420.22365285732201828No Hit
GGTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTAT6240.21738221646252245No Hit
GTTATAAATGGCTTAACTTCCTCTTTAGGAATCTCAGCGATCTTTTGTTC6060.21111157560302662No Hit
GTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGA6010.20936973091983335No Hit
CTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTAC5990.20867299304655604No Hit
CTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAAT5960.20762788623664005No Hit
GTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAGA5890.20518930368016944No Hit
GTGTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTG5790.20170561431378287No Hit
ATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAA5790.20170561431378287No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT5790.20170561431378287No Hit
ATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGAGTTGT5730.19961540069395092No Hit
CCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAA5600.19508660451764837No Hit
GTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACAT5520.1922996530245391No Hit
CTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGAC5490.19125454621462312No Hit
CTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCA5450.18986107046806852No Hit
GTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTT5320.18533227429176596No Hit
GTCTTTGCCTCCTCTACAGTGTAACCATTTAAACCCTGACCCGGGTAAGT5310.1849839053551273No Hit
ATTCTATACCTAGTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAG5300.18463553641848862No Hit
GTGTTACATAGCCAAGTGGCATTGTAACAAGAGTTTCATTTAGATCGTTA5290.18428716748184998No Hit
GTCTTAAGATTGTCAAAGGTGATAACTTCACCATCTAGGTGGAATGTGGT5220.18184858492537936No Hit
CTCTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGG5170.18010674024218606No Hit
GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT5140.1790616334322701No Hit
ATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGAT5130.17871326449563146No Hit
ACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTC4990.17383609938269026No Hit
AATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTC4990.17383609938269026No Hit
CTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCC4930.1717458857628583No Hit
GTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTG4880.170004041079665No Hit
CTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAAT4810.1675654585231944No Hit
ATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCA4640.16164318660033722No Hit
GATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATA4610.16059807979042123No Hit
GCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTA4490.15641765255075735No Hit
GACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGT4430.1543274389309254No Hit
CATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAG4430.1543274389309254No Hit
CTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCACTTTGAGA4370.15223722531109346No Hit
GTAATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTG4270.1487535359447069No Hit
GGTTAATGTTGTCTACTGTTGTAAACACCTTAATAGTCCTCACTTCTCTC4250.14805679807142957No Hit
TCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACAT4240.1477084291347909No Hit
ATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACAAGAG4230.14736006019815226No Hit
GTTAAAACACCCTCTTGAACAACATCACCCACTATATATGGAGCATCTTT4200.14631495338823627No Hit
TTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTG4190.14596658445159763No Hit
GCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACA4190.14596658445159763No Hit
CAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAA4140.14422473976840433No Hit
ATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTCAGT4080.1421345261485724No Hit
GTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTAT4060.14143778827529507No Hit
TCTTTATAGGAACCAGCAAGTGAGATGGTTTCAATAAAATGTTCTTCAGG4010.13969594359210177No Hit
GATAATAATGGTGCAAGTAGAACTTCGTGCTGATTAAAATTTTCATAAGC4000.13934757465546313No Hit
TTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGG3900.13586388528907656No Hit
CCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAG3880.13516714741579922No Hit
TGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAG3850.13412204060588326No Hit
AATATACACTTTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTA3840.13377367166924461No Hit
GGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCAT3780.13168345804941264No Hit
GTTGTAACTTCTTCAACACAAGCTTTGATTTTCTTATCATCTTGTTTTCT3770.131335089112774No Hit
CAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAG3690.12854813761966474No Hit
TTTATCACCTCTCTTAAGAAATTCTATACCTAGTTGTGTAGATTGTCCAG3650.1271546618731101No Hit
CTCTTGAACAACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAG3650.1271546618731101No Hit
ACATCACCCACTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAAT3630.1264579239998328No Hit
GTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAAT3630.1264579239998328No Hit
GTTGTTATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATT3630.1264579239998328No Hit
GTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGG3590.12506444825327814No Hit
GACATGTACCTACCCAGAAAACTAGGATCAGTTGTGTGGTAGTACTCAAA3590.12506444825327814No Hit
TAACAATGCAGTGGCAAGATAACAGTTGTTATCTGCCCATTTAATAGAAG3550.12367097250672351No Hit
GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT3540.12332260357008486No Hit
CTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTT3520.12262586569680754No Hit
GTGGTAGGATTACTAGTGTAATATACACTTTTATCACCTCTCTTAAGAAA3510.12227749676016889No Hit
CTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAA3470.12088402101361426No Hit
GTTATATAATTGTCTGTTGGCACTTTTCTCAAAGCTTTCGCTAGCATTTC3470.12088402101361426No Hit
GACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATG3430.11949054526705963No Hit
TGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTG3430.11949054526705963No Hit
CTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAA3420.11914217633042098No Hit
ATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGGCA3350.11670359377395037No Hit
ACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAAC3350.11670359377395037No Hit
GTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAG3330.11600685590067304No Hit
CCATTAACTTGTGGGTATTTCCACTTTTTAGTGTGATTTAATGCTGACAT3310.11531011802739574No Hit
GAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAA3300.11496174909075708No Hit
GTTGTGTGGTAGTACTCAAAAGCCTCAACACGTAGAGTGTCATCATTAGG3290.11461338015411843No Hit
AGATAATAGATGGTAGAATGTAAAAGGCACTTTTACACTTTTTAAGCACT3280.11426501121747976No Hit
CTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACA3270.1139166422808411No Hit
GGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCA3270.1139166422808411No Hit
GTTGTGTAGATTGTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGT3240.11287153547092513No Hit
GCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTT3230.11252316653428647No Hit
GTCCATATGTCATTGACATGTCCACAACTTGCGTGTGGAGGTTAATGTTG3220.11217479759764783No Hit
GTCCACAACTTGCGTGTGGAGGTTAATGTTGTCTACTGTTGTAAACACCT3190.11112969078773184No Hit
TGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAA3170.11043295291445453No Hit
TGTCAATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTCTTCCAGA3140.10938784610453854No Hit
GGATTAAACTTCAACTCTATTTGTTGGAGTGTTAACAATGCAGTGGCAAG3070.10694926354806794No Hit
AATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAG3060.10660089461142928No Hit
GTATAACCACAGCAGTTAAAACACCCTCTTGAACAACATCACCCACTATA3060.10660089461142928No Hit
GCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGA3060.10660089461142928No Hit
TCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTA3030.10555578780151331No Hit
ATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTT3000.10451068099159733No Hit
GGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTA3000.10451068099159733No Hit
TTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAA2970.10346557418168137No Hit
GTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACC2950.10276883630840405No Hit
TGTGTAGATTGTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGA2920.10172372949848807No Hit
ACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTG2920.10172372949848807No Hit
CCATTAATGTCAATATAAAGTAACAAGTTTTCTGTGAGGAACTTAGTTTC2910.10137536056184943No Hit
CTTGTATTTTAATACCCTTATATTTACGCTGTATAGTTGAAACTATGGCT2900.10102699162521077No Hit
CATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTT2890.10067862268857211No Hit
CTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCT2880.10033025375193345No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTT100.0070882486144.999989
TACATCA100.0070882486144.999986
ATATCTT100.0070882486144.999988
AGCGGGT203.8632195E-4108.749999
CTACACT203.8632195E-4108.749995
TAGAGCA850.093.8235244
TAGCGGG259.379881E-487.08
GTAGAGC1000.079.753
ATACATC300.001934331672.55
GGATTAA1350.064.444441
AGAGCAG1300.061.3461535
ATTAAAC1550.060.8064543
TTCAGAC2050.060.1219522
AAATTAG753.751702E-758.05
CAGACTA1800.056.3888854
TGTTCAG1450.055.05
TCAGACT1950.052.0512853
GTTCAGA2400.051.3541681
ACTTCAA1700.051.1764688
TATATAT10750.048.5581442
Produced by FastQC (version 0.11.5)