FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133524
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC10470.7841286959647704No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC9900.7414397411701268No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT9250.6927593541236032No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT7070.5294928252598784No Hit
GTTTCAAGAGTGCGGGAGAAAATTGATCGTACAACACGAGCAGCCTCTGA6990.5235013930079986No Hit
GAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCC5420.40591953506485723No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT5340.3999281028129774No Hit
CTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTG4970.3722177286480333No Hit
GCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTC4460.3340223480422995No Hit
TCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATT4190.31380126419220516No Hit
GTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAAC4000.29957161259399057No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG3900.2920823222791408No Hit
TCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATG3820.286090890027261No Hit
AACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCT3780.2830951739013211No Hit
ATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAACAT3760.2815973158383511No Hit
CAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTA3640.2726101674605314No Hit
TGCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCAC3250.24340193523261733No Hit
CCAATATTCCAGGCACCTTTTTTAGCTTTTCCTTTTGTAACTTTAAAATT3210.24040621910667745No Hit
GGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGA3170.23741050298073754No Hit
CAACTGAACAACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAG3140.23516371588628263No Hit
GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC3120.23366585782331264No Hit
TGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTA3030.22692549653994787No Hit
ACATAAGAGAACACACAGCCTCCAAAGGCAATAGTGCGACCACCCTTACG2990.22392978041400796No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT2880.21569156106767323No Hit
GTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGT2860.21419370300470325No Hit
GTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTAAAACACGCACA2840.21269584494173333No Hit
CTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTT2690.2014619094694587No Hit
GTGTATGCCCCTCCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACT2660.19921512237500374No Hit
CTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTA2660.19921512237500374No Hit
CAACTAGATTGTTAGTAGCCAAATCAGATGTGAACATCATAGCATCAATG2650.19846619334351875No Hit
GTCTTGATTATGGAATTTAAGGGAAATACAAAATTTGGACATTCCCCATT2650.19846619334351875No Hit
CTTCATGAGAGTTGAAAGGCACATTTGGTTGCATTCATTTGGTGACGCAA2580.19322369012312393No Hit
GTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAA2580.19322369012312393No Hit
GCAGTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAG2540.19022797399718402No Hit
TTATAATCCAAACCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGA2530.18947904496569906No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2490.18648332883975913No Hit
GTGCCAAGCTCGTCGCCTAAGTCAAATGACTTTAGATCGGCGCCGTAACT2470.1849854707767892No Hit
GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA2460.1842365417453042No Hit
CATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGT2430.18198975465084927No Hit
GTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATC2420.1812408256193643No Hit
GTCTCAGTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTC2390.1789940385249094No Hit
GCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTT2340.17524939336748452No Hit
GGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACC2320.17375153530451454No Hit
AAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGG2300.17225367724154458No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT2290.17150474821005962No Hit
ACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGTTATGGC2290.17150474821005962No Hit
GGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGA2250.16850903208411971No Hit
GGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAG2180.1632665288637249No Hit
GCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTATTGTTAT2170.1625175998322399No Hit
GTATTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTT2170.1625175998322399No Hit
GTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACG2090.1565261675803601No Hit
GTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATA2080.15577723854887512No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA2080.15577723854887512No Hit
TTTCAGAACGTTCCGTGTACCAAGCAATTTCATGCTCATGTTCACGGCAG2070.15502830951739013No Hit
CCTGTATGGTTACAACCTATGTTAGCGCTAGCACGTGGAACCCAATAGGC2060.15427938048590517No Hit
GTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGA2030.15203259339145023No Hit
CTGCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGT2010.15053473532848027No Hit
ACCATAACATGACCATGAGGTGCAGTTCGAGCATCCGAACGTTTGATGAA2010.15053473532848027No Hit
AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA2010.15053473532848027No Hit
ACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGG1980.14828794823402533No Hit
CATGAGGGACAAGGACACCAAGTGTCTCACCACTACGACCGTACTGAATG1950.1460411611395704No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT1940.14529223210808542No Hit
CTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACG1930.14454330307660046No Hit
GCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTC1900.14229651598214554No Hit
CTCAAGAGGGTAGCCATCAGGGCCACAGAAGTTGTTATCGACATAGCGAG1900.14229651598214554No Hit
GTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAG1890.14154758695066055No Hit
GCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGTGC1870.1400497288876906No Hit
AATCAGATGTGAACATCATAGCATCAATGAGTCTCAGTGAATACTGTGAA1860.13930079985620564No Hit
ACATAGGGCTGTTCAAGTTGAGGCAAAACGCCTTTTTCAACTTCTACTAA1860.13930079985620564No Hit
CTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTT1830.1370540127617507No Hit
CAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCG1820.1363050837302657No Hit
CCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACT1820.1363050837302657No Hit
ACACCACCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATC1820.1363050837302657No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT1800.13480722566729578No Hit
GTTTAAAGTCACCAACAATATTGATGTTGACTTTCTCTTTTTGGAGTATT1800.13480722566729578No Hit
GCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAAC1800.13480722566729578No Hit
CCTTTATTACCGTTCTTACGAAGAAGAACCTTGCGGTAAGCCACTGGTAT1800.13480722566729578No Hit
CCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTC1790.13405829663581081No Hit
TTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCA1770.13256043857284083No Hit
CTTGTAGATCTGTTCTCTAAACGAACATGAAAATTATTCTTTTCTTGGCA1750.13106258050987088No Hit
TCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTG1750.13106258050987088No Hit
CTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGCATGAAGCTTT1730.12956472244690093No Hit
CCGTTAAGCTCACGCATGAGTTCACGGGTAACACCACTGCTATGTTTAGT1710.12806686438393097No Hit
GGTAACACCACTGCTATGTTTAGTGTTCCAGTTTTCTTGAAAATCTTCAT1710.12806686438393097No Hit
GCATAAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCACC1710.12806686438393097No Hit
GGATTCAACAATTTGTTTGAATGCTTTATAATCCAAACCTTTCACAGTTT1700.127317935352446No Hit
ATGTTAGCGCTAGCACGTGGAACCCAATAGGCACACTTGTTATGGCAACC1700.127317935352446No Hit
ACTATGCTCAGGTCCTACTTCTGAATTGTGACATGCTGGACAATAAATTT1690.12656900632096102No Hit
TGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGAC1670.12507114825799107No Hit
ATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGT1660.1243222192265061No Hit
GTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCG1650.12357329019502111No Hit
CATTAAGACCTTCGGAACCTTCTCCAACAACACCTGTATGGTTACAACCT1640.12282436116353615No Hit
GTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAG1630.12207543213205117No Hit
AACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCA1630.12207543213205117No Hit
CTGTAAAACACGCACAGAATTTTGAGCAGTTTCAAGAGTGCGGGAGAAAA1620.12132650310056618No Hit
TCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACT1620.12132650310056618No Hit
ATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTG1610.12057757406908122No Hit
CATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTT1600.11982864503759624No Hit
ATATTGATGTTGACTTTCTCTTTTTGGAGTATTTCAAGAAGGTTGTCATT1590.11907971600611125No Hit
GATGTGAACATCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCC1580.11833078697462629No Hit
GAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCT1580.11833078697462629No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA1580.11833078697462629No Hit
GATAAGACCTCCTCCACGGAGTCTCCAAAGCCACGTACGAGCACGTCGCG1560.11683292891165632No Hit
CAGTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTC1550.11608399988017135No Hit
CGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGG1550.11608399988017135No Hit
GTCTTTAATGCACTCAAGAGGGTAGCCATCAGGGCCACAGAAGTTGTTAT1540.11533507084868638No Hit
ATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAACATCATAGCATC1540.11533507084868638No Hit
ATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCT1530.11458614181720139No Hit
GTTGAATAGTCTTGATTATGGAATTTAAGGGAAATACAAAATTTGGACAT1520.11383721278571642No Hit
GTTTAGTGTTCCAGTTTTCTTGAAAATCTTCATAAGGATCAGTGCCAAGC1510.11308828375423145No Hit
CGGCAAGACTATGCTCAGGTCCTACTTCTGAATTGTGACATGCTGGACAA1500.11233935472274648No Hit
CCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCA1500.11233935472274648No Hit
GTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCA1480.11084149665977652No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG1470.11009256762829155No Hit
AAAGAGGACTCAGTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTT1470.11009256762829155No Hit
GGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCG1460.10934363859680656No Hit
CCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGCATGAAGC1460.10934363859680656No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG1460.10934363859680656No Hit
GGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGTGAACATCATAG1450.10859470956532159No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1440.10784578053383662No Hit
TCCGTGTACCAAGCAATTTCATGCTCATGTTCACGGCAGCAGTATACACC1440.10784578053383662No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG1440.10784578053383662No Hit
ATCTAGTATTGTTATAGCGGCCTTCTGTAAAACACGCACAGAATTTTGAG1430.10709685150235163No Hit
GTTAGTAGCCAAATCAGATGTGAACATCATAGCATCAATGAGTCTCAGTG1420.10634792247086666No Hit
GAAGTCAACTGAACAACACCACCTGTAATGTAGGCCATTACAACTAGATT1420.10634792247086666No Hit
CTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGC1420.10634792247086666No Hit
CCTTTCACAGTTTCCACAAAAGCACTTGTGGAAGCAGAAAAAGATGCCAA1420.10634792247086666No Hit
ATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCT1420.10634792247086666No Hit
TGGTTGAATAGTCTTGATTATGGAATTTAAGGGAAATACAAAATTTGGAC1410.10559899343938169No Hit
GGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCG1410.10559899343938169No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA1400.1048500644078967No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA1400.1048500644078967No Hit
GTACAACACGAGCAGCCTCTGATGCAAATGCATAAAGAGGACTCAGTATT1400.1048500644078967No Hit
GTGAATACTGTGAAATTCCATCTAGTATTGTTATAGCGGCCTTCTGTAAA1390.10410113537641173No Hit
CATCATAGCATCAATGAGTCTCAGTGAATACTGTGAAATTCCATCTAGTA1390.10410113537641173No Hit
CACTTCATGAGAGTTGAAAGGCACATTTGGTTGCATTCATTTGGTGACGC1370.10260327731344179No Hit
CTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAG1350.10110541925047183No Hit
GATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGA1340.10035649021898686No Hit
TGGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTT1340.10035649021898686No Hit
CAAGAGGGTAGCCATCAGGGCCACAGAAGTTGTTATCGACATAGCGAGTG1340.10035649021898686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC100.00708403145.05
GAGCGCG100.00708403145.08
GTACTCG100.00708403145.08
AAGAGCG100.00708403145.06
CGCCAGC100.00708403145.08
GGTTATA100.00708403145.02
AGGTTAT100.00708403145.01
AGCGCGG100.00708403145.09
TACCCAG100.00708403145.04
TACTCGG100.00708403145.09
GGTACTC100.00708403145.07
AGCTTAC203.8586426E-4108.757
GGCGTGT203.8586426E-4108.758
ATAGGCG203.8586426E-4108.755
CTTACGC203.8586426E-4108.759
TTATACT301.594363E-596.666664
TGTAGAT2800.088.035713
CGCTTTC259.3688135E-487.0145
TTCTGAC259.3688135E-487.04
CTGACCA259.3688135E-487.06
Produced by FastQC (version 0.11.5)