FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-70-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-70-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42777
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGAGTAGCCATTCTTTTATTCCTTTACTTTCTTAATAAACTTGC3540.8275475138509012No Hit
CATTCTATTCTATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTC2260.5283212941534001No Hit
GTGCTAATACTGGTGCTGAAAAACATCCTGGAAATCAGTAACTTTGGAAA1990.46520326343595864No Hit
ATTCCATTCCATTGCATTCCCGTCCTCTCCATTTCATTAGCTTCCATTCC1920.4488393295462515No Hit
CATGAAGACAGTGTATCATTAAGAAATAACCATAAAGTGGGCAACCAGCA1660.3880590036701966No Hit
CATGAAGACAGTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACT1380.32260326811136825No Hit
GTGTATCATTAAGAAATAACCATAAAGTGGGCAACCAGCAGCCCTTGGGG1370.3202655632699815No Hit
GTGTAGGAATATGTTCACATAGTCACCCAAATGGAGCATCCTACTCTGTC1300.30390162938027443No Hit
GTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTGGG1270.2968885148561143No Hit
ACCATAAAGTGGGCAACCAGCAGCCCTTGGGGCTGCTCTGTCTATGGAGT1150.26883605675947353No Hit
CCCATTCTATTCTATTCCATTCCATTCCATTCCATTCCATTCCATTCCAT1110.25948523739392665No Hit
ATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGATAA1060.247796713186993No Hit
GCATACAGGTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGG1050.24545900834560627No Hit
GGAACATAATGGAATGGAATGGAATGGAATGGAATGGAATGGAATGGAAT920.2150688454075788No Hit
GTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGG870.20338032120064523No Hit
GTGTCATGGAGTGGAGTGGAATGGAATGGAACGGAATGCAGTGGAGTGGA870.20338032120064523No Hit
ATTCTATTCTATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCC850.19870491151787176No Hit
GAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAATCAATGAATCC850.19870491151787176No Hit
GTCCAGGAATTTATCCATTTCTTCTAGATTTTCTAGTTTATTTGCATACA850.19870491151787176No Hit
GTGCAGCACCGTGAGGTCCAGGGCTTCGAGTCGGCCACCTTCCTAGGCTA840.19636720667648502No Hit
CAATGTGTCCAGCCTGTAGCTTTCAAAATATCACCTAAGTGTGCTAATAC810.18935409215232485No Hit
GGTGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTG800.18701638731093811No Hit
CTCTTGGAGTCTGGATCAGGACCCCTCTCCTGTAACAATATGTATTTTTA730.17065245342123103No Hit
CTTCAGGACCATGTCCCATGAGTAAAATGCAGTCATGCTTCATGAATCTC720.1683147485798443No Hit
TCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTCAGGAC720.1683147485798443No Hit
TTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGAT710.1659770437384576No Hit
GCCCTTGGGGCTGCTCTGTCTATGGAGTAGCCATTCTTTTATTCCTTTAC710.1659770437384576No Hit
CTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGTGATAATCCCCTTTAT700.16363933889707086No Hit
ATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTCAGGA700.16363933889707086No Hit
GGTCCAGGATGAAGCAGTCGCCATTGTTGAAGCTCTCCCAGGACACAGGT670.15662622437291066No Hit
GTGTAGTCAGTGGGTGAAATTTATAATTTTAGAAGTTCCACACATGGAAA660.15428851953152395No Hit
TGCTAATACTGGTGCTGAAAAACATCCTGGAAATCAGTAACTTTGGAAAA640.1496131098487505No Hit
CATGAAGACAGTGTTTTGAGGTCCATTTAGGAAAAAAATCTACTAACTAC640.1496131098487505No Hit
CAGTGTAGCTGAGGAACGGAAATCTGCAGTATGACCTCCACTACTGGCTG630.14727540500736377No Hit
GTATGAGAGTTAATGCTGATAGAATTAAAAAGTATGAGTAAAGCAGAAAT610.14259999532459033No Hit
CCTGTAACAATATGTATTTTTAAACCTAATCCATCTTTCCCTAGCATGGG610.14259999532459033No Hit
ACTCTATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTT600.1402622904832036No Hit
GAGTTAATGCTGATAGAATTAAAAAGTATGAGTAAAGCAGAAATACAGCA590.13792458564181687No Hit
GTGTATGATTCTACTTATATGAAATATCTAGAAAAGGCATATTCATAGAG590.13792458564181687No Hit
ACTAAGATGAGAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCA590.13792458564181687No Hit
GTTTAAATCATATATTGGTGACATACTTATCACGAGGACAAGGGGGAAAA580.13558688080043013No Hit
TGTTTATAGTATTCTCTGTTGGTAGTTTGTATTTCTCTGGGATCAGTGGT570.1332491759590434No Hit
GGGTAACAGAGATTCATGAAGCATGACTGCATTTTACTCATGGGACATGG570.1332491759590434No Hit
GATGAGAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAAT560.13091147111765666No Hit
GTCTAAGGATAGAGTATGCGGAATGCACACTCAGAACACAGACACGTGCC560.13091147111765666No Hit
GTCCAACACCATGTCTGGCCTAGGGTAATTGTTCTTTCTAGTCTTTATTC550.12857376627626996No Hit
GAGCAAAACTCCATCTCAAAAAAACTGGAGAAGCAAGAGTAAACAAATTC550.12857376627626996No Hit
CCATATAGGAGCTCACTTCTTTTAATCCATAAGGCCATTTTCATAGGTTG540.12623606143488322No Hit
GCCTGTAGCTTTCAAAATATCACCTAAGTGTGCTAATACTGGTGCTGAAA540.12623606143488322No Hit
GTGGTGATAATCCCCTTTATCATTTTTTATTGTGTCTGTTTGATTCTTCT520.12156065175210977No Hit
GAGTAAACAAATTCAAAAGCTAGCAGAAGACAAGAAATAACTAAGATGAG510.11922294691072305No Hit
GCTCTGTCTATGGAGTAGCCATTCTTTTATTCCTTTACTTTCTTAATAAA490.11454753722794961No Hit
GTTCTGAATTATGTAAGTTCTTCAGGACCATGTCCCATGAGTAAAATGCA480.11220983238656287No Hit
GAAATAACTAAGATGAGAGCAGAACTGAAGGAGATAGAGACACGAAAAAC480.11220983238656287No Hit
CATGAAGACAGTGTAGGAATATGTTCACATAGTCACCCAAATGGAGCATC470.10987212754517614No Hit
GTCCTGAAGAACTTACATAATTCAGAACAGATTATTCCATAGTCTAAGGA470.10987212754517614No Hit
CTCTATCCTTAGACTATGGAATAATCTGTTCTGAATTATGTAAGTTCTTC470.10987212754517614No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG470.10987212754517614No Hit
GGATGAAGCAGTCGCCATTGTTGAAGCTCTCCCAGGACACAGGTACCTCG470.10987212754517614No Hit
GAGGTGTGGTGTGAAGTGGAATGGATTGGAATGGAATCAGATGTAATGGA460.1075344227037894No Hit
GTGTTCTGAGTGTGCATTCCGCATACTCTATCCTTAGACTATGGAATAAT460.1075344227037894No Hit
CTTTTATTCCTTTACTTTCTTAATAAACTTGCTTTCACTTTGCACTGCGG460.1075344227037894No Hit
CTCTTGGACACTGTGTCTAGATTTGTTCTTGCAGCTCTTCTGAAACACAC450.1051967178624027No Hit
ATACTAAACTGCTAAACAACTAGATGTAATCAGGTTATCAAAGGCAAGTA450.1051967178624027No Hit
ATATAAAACAGATAGTTGATGCACGATTAGGGAAAAATAAGGCAACCTAT450.1051967178624027No Hit
CCTATAGCCTTTTCTACCTGAAGATACTCTGTGTTTTAAAGCTAAGCTTA440.10285901302101597No Hit
GAGCAGAACTGAAGGAGATAGAGACACGAAAAACCCTTCAAAAATCAATG440.10285901302101597No Hit
CATTTAGAGACAAAGACAAACAAGAGAAATAGAGTTTTATTTTCTTAGCT430.10052130817962925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCC100.0070673055145.04
ATCTAAC100.0070673055145.01
GAGTATA100.0070673055145.01
ATAGAGC100.0070673055145.03
CCCCGTT100.0070673055145.07
GCAGGGT100.0070673055145.09
ACCCCGT100.0070673055145.06
TGAAGAC800.0126.8753
GATGTAG352.716406E-7103.571431
CAGTGTA850.0102.352949
ACAGTGT1150.088.260878
GTGTAGG259.3249616E-487.01
GACAGTG1600.072.57
GTAGGAA300.001923047572.53
AGACAGT1500.072.56
AAGACAG1650.070.303035
CAAGTTT350.003543200262.142857145
GGTATTA350.003543200262.142857145
AGATTGC350.003543200262.1428576
GATTGCT350.003543200262.1428577
Produced by FastQC (version 0.11.5)