FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-70-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-70-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64288
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCGTCGGCAGCGTCAGCACTTCAATTTCCATTTGACTCCTGGGGGAG12681.9723743155798903No Hit
CTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCT7221.1230711796913888No Hit
ATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAG6390.9939646590343455No Hit
CCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG4480.6968641114982579No Hit
GCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAGT4430.6890866102538576No Hit
CGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTA3840.5973120955699354No Hit
CTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGAT3260.5070930811348929No Hit
ACTTTCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG3240.5039820806371329No Hit
GCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACC3110.48376057740169237No Hit
GTAGTGTTCCTTAGGTAGGCGATCTCCTGTCATGGTTAGTGGGTGATCCC2990.46509457441513186No Hit
ACTTTCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGG2880.44798407167745147No Hit
GTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCA2780.43242906918865104No Hit
GGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCA2620.4075410652065704No Hit
CTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAAT2450.38109756097560976No Hit
TAACAAAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGA2340.3639870582379293No Hit
GTTCCTAGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACA2250.34998755599800896No Hit
GCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGAC2190.3406545545047287No Hit
ATCGTCGGCAGCGTCAGCACTTCAATTTCCATTTGACTCCTGGGGGAGTG2190.3406545545047287No Hit
CCCTAATTATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGA2180.3390990542558487No Hit
CCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTT2180.3390990542558487No Hit
CCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGG2070.32198855151816824No Hit
GTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCA1950.3033225485316078No Hit
CCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAA1920.2986560477849677No Hit
ACAAAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAA1850.2877675460428074No Hit
GTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTC1820.28310104529616725No Hit
AGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCA1790.27843454454952715No Hit
CCATCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGG1660.25821304131408657No Hit
CCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGG1640.25510204081632654No Hit
GGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACA1640.25510204081632654No Hit
CCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGT1570.24421353907416624No Hit
ACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAG1570.24421353907416624No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAA1490.23176953708312592No Hit
GTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGAC1480.23021403683424588No Hit
GGTATATGCTGCTCAAGCACAAGTCCCTCTGAGAAACATGCCATTCTGTG1440.22399203583872573No Hit
TGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACT1420.22088103534096565No Hit
GTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCA1400.21777003484320556No Hit
CTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGATGGT1320.20532603285216525No Hit
GTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAA1180.1835490293678447No Hit
ATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAA1170.18199352911896466No Hit
AAGCACTCGTGGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTG1150.17888252862120457No Hit
GTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGT1110.17266052762568443No Hit
GCTTAACCCTAATTATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGA1090.16954952712792434No Hit
CACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGAGTGA1090.16954952712792434No Hit
GATCACGCACTATCGTCGGCAGCGTCAGCACTTCAATTTCCATTTGACTC1060.16488302638128421No Hit
ACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCATCCAC1040.16177202588352416No Hit
GGTATATGCCACCACACTCAGCTAATTTTGGCATTTTTAGTAGAGACAGG1020.15866102538576407No Hit
CTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTC1010.15710552513688403No Hit
ACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGG1000.15555002488800398No Hit
GTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGTGGTGTCAC990.15399452463912394No Hit
CCTAGTAACCATAATAGGAGATTGCCTAGTAACCATAACAGGAGATCACC980.1524390243902439No Hit
ATCACGCACTATCGTCGGCAGCGTCAGCACTTCAATTTCCATTTGACTCC920.14310602289696367No Hit
CCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGGATGGTG920.14310602289696367No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT900.13999502239920358No Hit
GTGCAGGTATATGCCACCACACTCAGCTAATTTTGGCATTTTTAGTAGAG890.13843952215032354No Hit
ACTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAA880.13688402190144353No Hit
GCGTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCAATTTCC850.1322175211548034No Hit
TGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAG840.13066202090592335No Hit
GAGTGACACCACCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTG830.12910652065704328No Hit
GGGGGAGTGACACCACCATCATCCACGAGTGCTTTGTTAAGCGTCAATTT820.12755102040816327No Hit
CACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAACAAAGCACTCGTGG820.12755102040816327No Hit
TCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG810.12599552015928323No Hit
CAAACCAACCAACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCA780.1213290194126431No Hit
TTCCTAGGTAGTAGAAATCAATTTCCATTTGACTCCTGGGGGAGTGACAC770.11977351916376305No Hit
CTTTCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGGA750.11666251866600298No Hit
GTAATGGTCATTGTAGTGGTGAAGATGATGGTGGTAGTGGTGATGGTGGT730.1135515181682429No Hit
CTCGTGGATGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGAC720.11199601791936287No Hit
ATTTCCATTTGACTCCTGGGGGAGTGACACCACCATCCACGAGTGCTTTG720.11199601791936287No Hit
CTCGTGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCTTAAC690.10732951717272274No Hit
CTCCTGGGGGAGTGACACCACCATCATCCACGAGTGCTTTGTTAAGCGTC690.10732951717272274No Hit
GGGGGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGGGAGTGACA690.10732951717272274No Hit
CTCCCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCCCCCAGGAG680.10577401692384271No Hit
CCAACCAACTTTCAATTTCCATTTGACTCCTGGGGGAGTGACACCACCAT660.10266301642608262No Hit
AACTCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCCTGGGGG660.10266301642608262No Hit
CCACCATCATCCACGAGTGCTTTGTTAAGCGTCAATTTCCATTTGACTCC650.10110751617720258No Hit
GAAATTGATGGTGGTGTCACTCCCCCAGGAGTCAAATGGAAATTGACGCT650.10110751617720258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC100.0070755375145.08
ACGCACA151.2246968E-4145.05
GTAGAGC100.0070755375145.03
GGGAACG151.2246968E-4145.07
GGAACGT151.2246968E-4145.08
GTTCCTA400.0145.01
GACATGC100.0070755375145.01
CGTCGGT100.0070755375145.05
CGCACTG100.0070755375145.06
CGGTAGC100.0070755375145.08
TAAGCAG100.0070755375145.05
GCAGGTA301.0899021E-7120.8333363
CTATCGT1950.0115.256411
TATCGTC2000.0112.3752
GAACGTG203.8494327E-4108.759
AATAGGG203.8494327E-4108.753
TCGTCGG2250.099.888894
ATCGTCG2300.097.7173843
TATGCTG607.2759576E-1296.6666645
ACTTTCC750.096.6666641
Produced by FastQC (version 0.11.5)