FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-69-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-69-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178626
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA37712.1111148433038864No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT26011.4561150112525612No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA24391.3654227268146855No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA21701.214828748334509No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG20701.1588458567061906No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC15930.8918074636391118No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG15790.8839698588111473No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA14110.7899186008755724No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC13090.7328160514146876No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT13000.727777591168139No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT12450.6969870007725639No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA12010.6723545284561038No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC11930.6678758971258384No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT10840.6068545452509713No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG10840.6068545452509713No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC10660.596777624757874No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT10330.578303270520529No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG9370.5245596945573433No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG9100.5094443138176973No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT8690.48649132825008673No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA8180.45794005351964434No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA7180.401957161891326No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG7090.3969187016447774No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA6970.39020075464937914No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT6890.38572212331911365No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT6880.3851622944028305No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT6860.38404263657026416No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT6860.38404263657026416No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG6750.37788451849114907No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA6730.3767648606585827No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG6630.3711665714957509No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC6580.36836742691433494No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC6460.3616494799189368No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC6450.3610896510026536No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT6340.3549315329235386No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA6280.3515725594258395No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT6280.3515725594258395No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC6070.3398161521838926No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT5860.32805974494194573No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT5740.32134179794654755No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT5690.3185426533651316No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA5680.3179828244488484No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC5610.3140640220348661No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC5450.3051067593743352No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC5420.30342727262548563No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG5380.3011879569603529No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT5370.3006281280440697No Hit
AAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGT5260.2944700099649547No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTA5200.2911110364672556No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA5030.2815939448904415No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT4760.2664785641507955No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC4690.2625597617368132No Hit
CATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCA4610.2580811304065478No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT4470.25024352557858315No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA4420.24744438099716728No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA4310.24128626291805225No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA4240.23736746050406995No Hit
ATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTG4220.23624780267150358No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT4210.23568797375522038No Hit
GTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGC4170.23344865809008766No Hit
GTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCT4040.2261708821784063No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA3980.22281190868070716No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3950.22113242193185761No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA3920.2194529351830081No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA3900.2183332773504417No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT3900.2183332773504417No Hit
CTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGT3790.21217515927132669No Hit
TTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTG3740.20937601468991074No Hit
CCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGT3710.20769652794106122No Hit
GTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGG3670.20545721227592847No Hit
GTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTT3600.20153840986194618No Hit
CCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC3570.1998589231130966No Hit
ATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAA3520.19705977853168075No Hit
CTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACG3490.19538029178283117No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA3480.19482046286654797No Hit
GAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTAT3460.19370080503398163No Hit
GTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGA3450.19314097611769843No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC3380.18922217370371613No Hit
GGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTA3370.18866234478743296No Hit
GATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACT3350.1875426869548666No Hit
GCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACC3340.18698285803858342No Hit
TAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCT3330.18642302912230022No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT3280.1836238845408843No Hit
ATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTAC3270.18306405562460112No Hit
GTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAAT3230.1808247399594684No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT3190.17858542429433566No Hit
GGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATA3170.1774657664617693No Hit
TCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACAC3110.1741067929640702No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA3110.1741067929640702No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG3070.17186747729893745No Hit
CTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTT2910.16291021463840652No Hit
GCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCAT2840.1589914122244242No Hit
TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTT2830.15843158330814103No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC2820.15787175439185785No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2810.15731192547557465No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2800.15675209655929148No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2790.1561922676430083No Hit
AGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACAC2760.15451278089415874No Hit
GAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAAT2740.1533931230615924No Hit
GTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGA2700.15115380739645964No Hit
AATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTA2630.14723500498247735No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC2610.14611534714991098No Hit
TATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAAC2600.1455555182336278No Hit
ATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTG2580.14443586040106143No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTC2570.14387603148477826No Hit
CTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTG2570.14387603148477826No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG2560.14331620256849506No Hit
ATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTG2560.14331620256849506No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT2530.14163671581964551No Hit
GTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT2510.14051705798707914No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTT2480.1388375712382296No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG2460.13771791340566322No Hit
ACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT2410.1349187688242473No Hit
CTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTA2380.13323928207539776No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC2370.13267945315911459No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA2360.13211962424283138No Hit
GTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGA2320.12988030857769864No Hit
TTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGA2320.12988030857769864No Hit
AATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAGTT2290.12820082182884912No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2280.12764099291256592No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG2270.12708116399628272No Hit
CCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAA2250.12596150616371637No Hit
ATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAA2230.12484184833115No Hit
GAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAG2220.12428201941486683No Hit
GTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCC2160.12092304591716772No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA2160.12092304591716772No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA2160.12092304591716772No Hit
TTACATTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAA2150.12036321700088454No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG2150.12036321700088454No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2130.11924355916831815No Hit
ACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGT2120.11868373025203498No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2110.11812390133575179No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA2100.11756407241946862No Hit
TAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTG2070.11588458567061904No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT2050.11476492783805269No Hit
CTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACAT2030.11364527000548633No Hit
CTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTA2000.11196578325663677No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT1990.11140595434035358No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG1990.11140595434035358No Hit
CTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATAC1990.11140595434035358No Hit
GGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATAC1990.11140595434035358No Hit
ATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAG1970.11028629650778722No Hit
AAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAATGTAAA1940.10860680975893766No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA1930.10804698084265447No Hit
ACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATT1920.1074871519263713No Hit
CGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCA1920.1074871519263713No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC1890.10580766517752176No Hit
TTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGT1890.10580766517752176No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA1880.10524783626123856No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT1820.10188886276353945No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAATGTT1820.10188886276353945No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC1810.10132903384725628No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTT100.007086022145.0145
GTTCCTA306.475602E-10145.01
GCAGCGG100.007086022145.07
AGCGGGG100.007086022145.09
GATCCGT202.1257674E-6145.07
TAATATC100.007086022145.06
TCCGTTC202.1257674E-6145.09
TGGAACT100.007086022145.0145
ATCCGTT202.1257674E-6145.08
CAGCGGG100.007086022145.08
AGATCCG202.1257674E-6145.06
TAGATCC202.1257674E-6145.05
TCCTAGG404.793037E-9108.753
GCAGGTA203.860803E-4108.753
GTAAGCC203.860803E-4108.75145
AGGTATA203.860803E-4108.755
CAGGTAT203.860803E-4108.754
CTAGGTA502.2579115E-887.05
TGCAGGT259.374037E-487.02
CCTAGGT502.2579115E-887.04
Produced by FastQC (version 0.11.5)