FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-68-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-68-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235123
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA31341.3329193656086389No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT25351.0781590911990746No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA23691.0075577463710483No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA19020.8089383003789505No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT18160.7723617000463587No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG17580.7476937602871688No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG16780.7136690157917345No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA16480.7009097366059467No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC15880.6753911782343709No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC13870.5899040076895923No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC12690.5397175095588266No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT12570.5346137978845115No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT12410.5278088489854247No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA11700.49761188824572666No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT11370.48357668114136004No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA11330.4818754439165883No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT11060.47039209264937926No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT10840.4610352879131348No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC10480.4457241528901894No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG9960.4236080689681571No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA9950.4231827596619641No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA9730.4138259549257197No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT9710.41297533631333383No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT9400.39979074782135304No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC9150.38915801516652987No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG9060.3853302314107935No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC8740.37172033361261975No Hit
CTTGTAAATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT8690.36959378708165513No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG8610.3661913126321117No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT8560.3640647661011471No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA8550.36363945679495413No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT8270.3517307962215521No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC8160.3470523938534299No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG8070.34322461009769356No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC7770.3304653309119057No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC7640.3249363099313976No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA7610.3236603820128188No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA7590.32280976340043294No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA7440.316430123807539No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC7310.310901102827031No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT7240.3079239376836804No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA6890.2930381119669279No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA6720.2858078537616482No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA6490.2760257397192108No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA6480.27560043041301785No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA6220.26454238845200173No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG6190.2632664605334229No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG5900.250932490653828No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT5890.25050718134763506No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT5810.24710470689809166No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT5740.24412754175474113No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT5690.2420009952237765No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA5680.24157568591758358No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT5660.24072506730519772No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG5560.23647197424326843No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT5500.23392011840611082No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC5420.2305176439565674No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT5290.22498862297605934No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT5250.2232873857512876No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT5130.21818367407697245No Hit
AAATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGT5050.21478119962742903No Hit
CACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCA5040.21435589032123611No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA5000.21265465309646442No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT4980.21180403448407856No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG4890.2079762507283422No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC4870.20712563211595633No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA4730.20117130182925533No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT4660.19819413668590485No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT4620.1964928994611331No Hit
ATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTT4490.19096387848062504No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT4490.19096387848062504No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA4400.18713609472488868No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT4370.18586016680630987No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA4330.18415892958153818No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT4320.18373362027534523No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA4270.1816070737443806No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA4260.18118176443818768No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC4220.17948052721341595No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG4200.1786299086010301No Hit
TTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTC4180.17777928998864423No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA4160.1769286713762584No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG4020.17097434108955736No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTA3990.1696984131709786No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA3980.16927310386478567No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG3900.16587062941524222No Hit
TTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTG3890.1654453201090493No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT3880.1650200108028564No Hit
ATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCC3870.16459470149666344No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA3780.16076691774092708No Hit
ATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTG3730.15864037120996244No Hit
GTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTT3590.15268604092326143No Hit
CATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCA3550.1509848036984897No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG3530.15013418508610388No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC3520.14970887577991093No Hit
CTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTG3380.14375454549320993No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3340.14205330826843823No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG3310.14077738034985945No Hit
CTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGT3280.13950145243128065No Hit
GACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCAC3250.13822552451270187No Hit
GTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCT3240.13780021520650892No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC3240.13780021520650892No Hit
GAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTAT3230.137374905900316No Hit
CTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACG3220.13694959659412306No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC3220.13694959659412306No Hit
GGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTA3200.1360989779817372No Hit
CCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACC3190.13567366867554428No Hit
ATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAA3170.13482305006315842No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3170.13482305006315842No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT3150.13397243145077256No Hit
GGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCT3110.13227119422600087No Hit
ACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTAATAAAC3100.13184588491980792No Hit
ACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATT3100.13184588491980792No Hit
GTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATT3040.12929402908265036No Hit
CTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTT3030.12886871977645742No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2970.12631686393929983No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2930.12461562671452814No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT2920.12419031740833521No Hit
ACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTC2900.12333969879594936No Hit
GTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAAT2900.12333969879594936No Hit
GTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGC2890.12291438948975643No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC2860.12163846157117764No Hit
TGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTT2840.12078784295879179No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA2830.12036253365259886No Hit
GTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCCATCCAACT2830.12036253365259886No Hit
ACCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCT2830.12036253365259886No Hit
TAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCT2810.119511915040213No Hit
GTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGA2800.11908660573402005No Hit
GCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACC2790.11866129642782715No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA2770.11781067781544129No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT2750.11696005920305541No Hit
GTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGG2750.11696005920305541No Hit
AAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATA2740.11653474989686248No Hit
GATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACT2720.11568413128447665No Hit
CTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTA2700.11483351267209077No Hit
GAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTT2680.11398289405970491No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2670.11355758475351199No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2660.11313227544731906No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA2650.11270696614112613No Hit
TAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAG2650.11270696614112613No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2640.1122816568349332No Hit
AATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTA2640.1122816568349332No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT2640.1122816568349332No Hit
ACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTT2600.11058041961016149No Hit
AATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCG2590.11015511030396856No Hit
ATGTAAAACTGAGGATCTGAAAACTTTGTCAGGGTAATAAACACCACGTG2580.10972980099777563No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA2580.10972980099777563No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTTTCGATTCAGATCT2570.1093044916915827No Hit
ATAAACACCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTAC2540.10802856377300392No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC2540.10802856377300392No Hit
ATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAG2520.10717794516061806No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC2520.10717794516061806No Hit
ATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTG2510.10675263585442513No Hit
GTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTT2490.10590201724203928No Hit
GTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGT2490.10590201724203928No Hit
ACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAG2490.10590201724203928No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2480.10547670793584635No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2470.10505139862965342No Hit
ACCCTGTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAA2470.10505139862965342No Hit
GTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACATTGGA2430.10335016140488171No Hit
GTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAG2420.10292485209868878No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2410.10249954279249585No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA2390.10164892418011No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2380.10122361487391707No Hit
GAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGTTGAAT2370.10079830556772414No Hit
CAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTG2360.10037299626153119No Hit
CCATCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGT2360.10037299626153119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT351.0913936E-11145.04
TAGCCTG151.2288181E-4145.0145
ATCCGTT100.007087438145.08
ACTACAT100.007087438145.09
TAAGATA151.2288181E-4145.06
CCTTGCA202.1271826E-6145.09
TCTATTA100.007087438145.04
GTCAGAC301.0990698E-7120.8333369
TAGAGCA256.454511E-6116.02
GTAGAGC256.454511E-6116.01
CCCTTGC256.454511E-6116.08
TTCCTAG203.86234E-4108.751
CTAGCCG203.86234E-4108.759
CTTGTAA1250.0104.41
AGGTATA501.9463187E-10101.55
CCCCTTG301.596903E-596.6666647
GCAGGTA554.1472958E-1092.2727363
CCCTAAT1000.087.01
TCCTAGG259.377755E-487.02
ATAAGAT259.377755E-487.05
Produced by FastQC (version 0.11.5)