FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-67-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-67-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102531
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG18441.7984804595683257No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG18071.7623938126030174No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC17501.7068008699807864No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG10341.0084754854629332No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT8820.8602276384703162No Hit
GATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCA6560.6398064975470834No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC5920.5773863514449289No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC5560.5422750192624669No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG4890.476928928811774No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT4710.4593732627205431No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA4200.40963220879538864No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG4060.39597780183554243No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTG4040.3940271722698501No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA4030.39305185748700394No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC3900.3803727653100038No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT3580.34916269225892654No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA3450.33648360008192646No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA3450.33648360008192646No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA3270.3189279339906955No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG3200.31210073051077236No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3180.31015010094508005No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2970.28966849050531057No Hit
GTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTGCACTTACACCGCA2830.2760140835454643No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG2720.2652856209341565No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2670.2604090470199257No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2600.25358184354000257No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC2570.2506558991914641No Hit
CCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTT2250.2194458261403868No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2240.21847051135754064No Hit
GTACAGACTGTGTTTTTAAGTGTAAAACCCACAGGGTCATTAGCACAAGT2240.21847051135754064No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA2200.21456925222615597No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG2180.21261862266046366No Hit
GCCATAACCTTTCCACATACCGCAGACGGTACAGACTGTGTTTTTAAGTG2050.19993953048346355No Hit
GGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGC2050.19993953048346355No Hit
GTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGA2040.1989642157006174No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC1910.18628512352361726No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA1890.18433449395792492No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC1890.18433449395792492No Hit
GTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGCAACAGGACTAAGC1880.18335917917507877No Hit
GTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAAC1850.18043323482654028No Hit
GTTATTGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATT1840.1794579200436941No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA1830.17848260526084794No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA1820.1775072904780018No Hit
CACTTACACCGCAAACCCGTTTAAAAACGATTGTGCATCAGCTGACTGAA1800.17555666091230945No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT1800.17555666091230945No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA1800.17555666091230945No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG1790.17458134612946327No Hit
GATATATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATG1770.17263071656377096No Hit
GTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTA1760.17165540178092478No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT1750.17068008699807863No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT1740.16970477221523247No Hit
GTGCCGCACGGTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAA1740.16970477221523247No Hit
ATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTATGACA1720.16775414264954014No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA1710.16677882786669396No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC1710.16677882786669396No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT1700.1658035130838478No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA1690.16482819830100165No Hit
GTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTAC1690.16482819830100165No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA1680.16385288351815547No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA1660.16190225395246316No Hit
CCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTAC1660.16190225395246316No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC1650.16092693916961698No Hit
GTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAAC1630.15897630960392467No Hit
GTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCA1620.15800099482107852No Hit
TATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTA1610.15702568003823233No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC1610.15702568003823233No Hit
CCTACAAGGTGGTTCCAGTTCTGTATAGATAGTACCAGTTCCATCACTCT1600.15605036525538618No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA1600.15605036525538618No Hit
GTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAAT1590.15507505047254003No Hit
ACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGAC1590.15507505047254003No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC1570.1531244209068477No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT1560.15214910612400154No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT1560.15214910612400154No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA1550.15117379134115536No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATA1530.14922316177546302No Hit
CAACAGGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTT1530.14922316177546302No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC1530.14922316177546302No Hit
GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA1520.14824784699261687No Hit
CGGTACAGACTGTGTTTTTAAGTGTAAAACCCACAGGGTCATTAGCACAA1500.14629721742692453No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC1490.14532190264407838No Hit
GTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTT1470.14337127307838604No Hit
CATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTC1460.1423959582955399No Hit
GTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGG1460.1423959582955399No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1460.1423959582955399No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA1440.14044532872984755No Hit
GGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTG1440.14044532872984755No Hit
CCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATA1430.1394700139470014No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC1430.1394700139470014No Hit
GTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGC1410.13751938438130906No Hit
CTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATAG1410.13751938438130906No Hit
GGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCAC1410.13751938438130906No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT1400.1365440695984629No Hit
CAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAA1390.13556875481561673No Hit
CTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGA1370.1336181252499244No Hit
AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT1360.13264281046707824No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA1350.13166749568423208No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC1350.13166749568423208No Hit
ATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCT1350.13166749568423208No Hit
ATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCA1340.13069218090138593No Hit
ATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGT1340.13069218090138593No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA1340.13069218090138593No Hit
TTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC1340.13069218090138593No Hit
TCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA1340.13069218090138593No Hit
GTCTGTAACAAACCTACAAGGTGGTTCCAGTTCTGTATAGATAGTACCAG1340.13069218090138593No Hit
GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAAT1330.12971686611853978No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG1320.1287415513356936No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC1310.12776623655284744No Hit
CAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTA1310.12776623655284744No Hit
GAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGAT1300.12679092177000129No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCT1300.12679092177000129No Hit
TCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACAT1300.12679092177000129No Hit
GTTCTGTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCAT1290.1258156069871551No Hit
CATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTGCACTTACA1280.12484029220430895No Hit
ATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAA1260.12288966263861661No Hit
AGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGG1260.12288966263861661No Hit
TCTTAACACAATTAGTGATTGGTTGTCCCCCACTAGCTAGATAATCTTTG1260.12288966263861661No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA1260.12288966263861661No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG1250.12191434785577046No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA1250.12191434785577046No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTC1250.12191434785577046No Hit
ATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCC1240.12093903307292429No Hit
GATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAAATCCT1240.12093903307292429No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC1220.11898840350723196No Hit
GGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGT1210.11801308872438579No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT1210.11801308872438579No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC1200.11703777394153964No Hit
CCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCAT1180.1150871443758473No Hit
GACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGT1170.11411182959300115No Hit
ATATTGGCTTCCGGTGTAACTGTTATTGCCTGACCAGTACCAGTGTGTGT1170.11411182959300115No Hit
GTACAGACAACACGATGCACCACCAAAGGATTCTTGATCCATATTGGCTT1170.11411182959300115No Hit
CTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACAT1160.11313651481015498No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT1160.11313651481015498No Hit
GTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCCCCCACTAG1160.11313651481015498No Hit
GTACAAACCTACCTCCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCA1160.11313651481015498No Hit
TACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATA1150.11216120002730881No Hit
CACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAAC1150.11216120002730881No Hit
GTACCATACCTCTATTTAGGTTGTTTAATCCTTTAATAAAGTATAAATAC1150.11216120002730881No Hit
GCACAAGACATCTGTCGTAGTGCAACAGGACTAAGCTCATTATTCTGTAA1150.11216120002730881No Hit
CAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCG1150.11216120002730881No Hit
GAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTG1140.11118588524446264No Hit
CCATTAGTTTGGCTGCTGTTGTAAGAGGTATTATGTTCAAGGGAACACAA1140.11118588524446264No Hit
GCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATC1140.11118588524446264No Hit
ATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGT1140.11118588524446264No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC1130.11021057046161647No Hit
GTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCA1130.11021057046161647No Hit
GTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAA1130.11021057046161647No Hit
ATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTAAG1120.10923525567877032No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC1120.10923525567877032No Hit
AGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACT1090.10630931133023183No Hit
AGATAATACAGTTGAATTGGCAGGCACTTCTGTTGCATTACCAGCTTGTA1090.10630931133023183No Hit
CATGAAGACAGTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC1080.10533399654738566No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA1080.10533399654738566No Hit
ACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTAC1080.10533399654738566No Hit
AGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAA1070.1043586817645395No Hit
GGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGT1070.1043586817645395No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT1060.10338336698169333No Hit
AGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAAC1060.10338336698169333No Hit
GTGCAAGTACAAACCTACCTCCCTTTGTTGTGTTGTAGTAAGCTAACGCA1050.10240805219884716No Hit
TCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCA1050.10240805219884716No Hit
AACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGAT1050.10240805219884716No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA1050.10240805219884716No Hit
TCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAAC1040.10143273741600102No Hit
GTTACAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTC1040.10143273741600102No Hit
TCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTAT1030.10045742263315487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG100.007081647145.01
ACATGAC100.007081647145.0145
ACTCCTT100.007081647145.08
TAGCATT151.2268119E-4145.04
TAGCAAG100.007081647145.06
TGAAGAC253.6874553E-8145.03
GTTAGGA100.007081647145.03
GTGGCCA100.007081647145.03
CCTAGCA151.2268119E-4145.02
GCTCAAT100.007081647145.02
CCCTAGC151.2268119E-4145.01
GGTAATT100.007081647145.07
TGGCCAT100.007081647145.04
GCAAGAT203.856057E-4108.758
CTAGCAT203.856057E-4108.753
CATGAAG406.078135E-790.6251
GACAGTG406.078135E-790.6257
GTGTGGC259.362556E-487.01
TTGATTC1100.079.090904145
GACGGGC3950.078.924057
Produced by FastQC (version 0.11.5)