FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-67-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-67-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218131
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA15750.7220431758897177No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA11870.5441684125594254No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT10180.46669203368617945No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA9830.45064662977751907No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG9470.4341427857571826No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA8890.407553259279974No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG8340.3823390531377933No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC8130.37271181079259713No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC7990.36629364922913293No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG7920.3630845684474009No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC7900.3621676882240488No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA7510.3442885238686844No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC7390.3387872425285723No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT7360.3374119221935442No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC6800.31173927593968764No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT6650.30486267426454744No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT6250.28652506979750697No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA6180.2833159890157749No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT6090.2791900280106908No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC6030.2764393873406348No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA5830.26727058510711454No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT5540.2539758218685102No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT5470.2507667410867781No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC5280.2420563789649339No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT5230.23976417840655384No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT5170.2370135377364978No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC5170.2370135377364978No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT5160.23655509762482177No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC5130.23517977728979375No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC5130.23517977728979375No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC5120.23472133717811774No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG5010.22967849594968162No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA5010.22967849594968162No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA4970.22784473550297757No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA4930.22601097505627354No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA4840.22188501405118943No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG4750.21775905304610532No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT4730.2168421728227533No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT4620.21179933159431716No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT4520.20721493047755707No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT4470.204922729919177No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT4250.19483704746230476No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG4170.19116952656889666No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT4090.18750200567548858No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4050.18566824522878453No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG4020.1842929248937565No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA4010.18383448478208048No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG3890.17833320344196835No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC3880.17787476333029237No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC3860.17695788310694033No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA3850.17649944299526432No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC3690.16916440120844814No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA3660.1677890808734201No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT3530.16182935942163196No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC3500.1604540390866039No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA3470.1590787187515759No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA3450.15816183852822388No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT3410.15632807808151983No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT3370.15449431763481578No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG3320.15220211707643572No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT3320.15220211707643572No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3260.14945147640637965No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3230.1480761560713516No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT3170.14532551540129554No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA3090.14165799450788746No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG3090.14165799450788746No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT3080.14119955439621146No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG3060.14028267417285945No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC3040.1393657939495074No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT3040.1393657939495074No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA3040.1393657939495074No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT2960.1356982730560993No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG2920.13386451260939528No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC2900.13294763238604324No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA2890.13248919227436723No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT2880.13203075216269122No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG2870.13157231205101522No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA2830.12973855160431116No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT2820.12928011149263516No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC2810.12882167138095915No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2800.12836323126928312No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2800.12836323126928312No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT2740.12561259059922705No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT2690.12332039004084702No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2690.12332039004084702No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT2640.12102818948246695No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA2630.12056974937079096No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA2620.12011130925911494No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA2620.12011130925911494No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2600.11919442903576291No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG2580.11827754881241088No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT2570.11781910870073489No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA2570.11781910870073489No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA2570.11781910870073489No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC2550.11690222847738285No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA2540.11644378836570685No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG2530.11598534825403083No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT2500.11461002791900281No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT2470.11323470758397476No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2400.11002562680224269No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT2390.10956718669056667No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA2380.10910874657889066No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG2360.10819186635553865No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT2350.10773342624386263No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2320.1063581059088346No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT2300.10544122568548259No Hit
GTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAAC2280.10452434546213056No Hit
CTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTG2280.10452434546213056No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2260.10360746523877853No Hit
AAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTC2240.10269058501542652No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA2240.10269058501542652No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT2230.1022321449037505No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA2230.1022321449037505No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2230.1022321449037505No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA2220.1017737047920745No Hit
TTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCTCCAAGAGCTAG2210.10131526468039848No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG2210.10131526468039848No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2190.10039838445704645No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG2190.10039838445704645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG100.007087088145.01
GTTCCTA750.0125.666671
CCGTTCA301.0987969E-7120.833336145
TTCCTAG850.0110.8823552
TCCTAGG900.0104.722223
CCTAGGT1000.094.254
CAAATGG406.101345E-790.6253
TGGAAAT451.2296441E-680.555567
AATGGAA451.2296441E-680.555565
CTAGGTA1100.079.0909045
AAATTAG2400.072.55
ACCAACC608.00228E-872.53
GTAGATC4000.070.68754
TAGGTAG1250.069.66
TATAAAT2500.069.62
TGTAGAT4200.069.047623
GGCGTGT851.6916601E-1068.23538
TTGTAGA4400.065.909092
TAGATCT4300.064.069765
GAGCAGG350.00356276762.1428531
Produced by FastQC (version 0.11.5)