FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-66-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-66-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73237
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAAGACAGTGTGGGGAGAAAGCCAGACTCAGGGTGTTTATGTTTGTG2820.3850512718980843No Hit
GTGTAGACCTGCCAACAGCGTGATTTCAGACTTCTGACTTCCAGAAGCGT2430.3317995002526046No Hit
GTGTATGTGTGTGCACATGTGATACATGAGATACACTCACAACCGCATAG2140.2922020290290427No Hit
GTGTATAATAAAGTGCTAACGTAACAATAAGGTTAATAAGTTAACCCTCG1900.2594317080164398No Hit
GTATACACCCAAAGGATTATAAATCATTCTAGTATAAAGGCACATGCATA1680.22939224708822042No Hit
GTGTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCAATGCCTCCTG1680.22939224708822042No Hit
GTGTGGGGAGAAAGCCAGACTCAGGGTGTTTATGTTTGTGGGTAATGACA1640.2239305269194533No Hit
GCCAATGCTAGGGAGAGCTGCCACTAACCACACAGAGAGGAAGGAAAATT1440.19662192607561751No Hit
CTCTTAGGACTCTGCCTTATGAGCTACTGATCTCTTGAGCACTCACCAAA1400.19116020590685037No Hit
GTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCAATGCCTCCTGTA1300.17750590548493247No Hit
GCCTAAGGAGGCACTATGGAGCAGCAAGTTGGATCCCCCACTAGATGGCG1220.16658246514739816No Hit
ATTATACACTGATCCGAAACTTATATCTAAAATATTTGTACCAAAATCTT1180.16112074497863102No Hit
CATGAAGACAGTGTACTATGGGGGTATTAGTGAGGATGGAAAGAAATTCA1170.15975531493643924No Hit
GTGTTTATGTTTGTGGGTAATGACAGTCAGCTCTGGGCTCCAGATGATGC1160.15838988489424746No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1140.15565902480986388No Hit
CATGAAGACAGTGTGGTGATTCCTCAAGGATCTAGAACTAGAAATACCAC1130.15429359476767207No Hit
GTATAATAAAGTGCTAACGTAACAATAAGGTTAATAAGTTAACCCTCGAA1050.1433701544301378No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1010.13790843426137062No Hit
GTATATACCCAAAGGATTATAAATCATTCTACTATAAAGACACATGCACA1000.1365430042191788No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA970.13244671409260347No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA970.13244671409260347No Hit
CATGAAGACAGTGTAGACCTGCCAACAGCGTGATTTCAGACTTCTGACTT970.13244671409260347No Hit
GTGTACACTGACTACATACACAGTCTCTGAATGGGCAGAAGGCCAGACTA950.1297158540082199No Hit
CTACATACACAGTCTCTGAATGGGCAGAAGGCCAGACTAGTCTATGCCTT910.12425413383945275No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA880.12015784371287737No Hit
GTCTCTGAATGGGCAGAAGGCCAGACTAGTCTATGCCTTTTAAACACTTC880.12015784371287737No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG880.12015784371287737No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC830.11333069350191842No Hit
CATATACACCATGGAATACTGTGCAGCCATAAAAAAGGATGAGTTCATGT810.11059983341753486No Hit
ACTTATGAGTGAGAACATGTGGTGTTTGGTTTTCTGTTCTTGTGATAGTT810.11059983341753486No Hit
GTGTATATGTGCCACATTTTCTTTATCCAGTCTATTATTGATGGACATTT790.10786897333315128No Hit
GCCAATGCTAGGGAGGCTGCCACTAACCACACAGAGAGGAAGGAAAATTC790.10786897333315128No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT780.10650354329095948No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC760.1037726832065759No Hit
TCTTAGGACTCTGCCTTATGAGCTACTGATCTCTTGAGCACTCACCAAAT750.10240725316438412No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT740.10104182312219233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATA100.007077538145.000026
GTAGTAG306.4210326E-10145.03
CATGAAG2300.0138.695661
GGTAGTA351.877197E-9124.285712
TGAAGAC2750.0116.03
ATGAAGA2750.0116.02
GCCAATG256.424614E-6116.01
AGACAGT2850.0114.473696
GGATGCA203.8516015E-4108.750018
GGGTAGT404.7530193E-9108.750011
AGCGGTT203.8516015E-4108.750019
ACAGTGT3200.099.687518
GAAGACA3200.099.687514
GACCTGC607.2759576E-1296.6666646
CAGCGGT259.351782E-487.08
TTGTTTA259.351782E-487.01
CAGTGTG2350.086.382989
AAGACAG3850.082.857155
CCAATGC353.415995E-582.857142
GTGTACT353.415995E-582.857141
Produced by FastQC (version 0.11.5)