FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-66-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-66-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134788
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG15411.1432768495711785No Hit
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT11500.8531916787844614No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC11400.8457726207080749No Hit
CTTGTAGATCTGTTCTCTAAACGAACTAAATATTATATTAGTTTTTCTGT9470.7025847998338131No Hit
CGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATT9360.6944238359497877No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT8950.6640056978366027No Hit
CAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAG7640.5668160370359379No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA7450.5527198266908033No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC7160.5312045582692821No Hit
CTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACA6730.49930260854081965No Hit
CACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCA6650.4933673620797103No Hit
CTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACC6300.4674006588123572No Hit
CTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGAC6030.4473692020061133No Hit
ATGTTTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT5750.4265958393922307No Hit
ATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTTCG5490.40730628839362554No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC5360.39766151289432294No Hit
ATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGAC5170.38356530254918836No Hit
GTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAA4960.36798528058877644No Hit
GTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCC4950.3672433747811378No Hit
ATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACA4850.3598243167047512No Hit
GATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAAC4700.3486957295901712No Hit
AAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATG4560.33830904828323No Hit
GCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTG4440.329406178591566No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT4440.329406178591566No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC4340.3219871205151794No Hit
CATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCT3930.29156898240199425No Hit
GAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGA3840.28489183013324626No Hit
AAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAATTA3670.272279431403389No Hit
AGCTAGCAGAAGGCAAGAAATTAAGATCAGAGCAGAACTGAAAGAGACAG3580.2656022791346411No Hit
ATTAAGATCAGAGCAGAACTGAAAGAGACAGAGACACAAAAAAACCTTCA3550.2633765617117251No Hit
GCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACCAGCTCCTGGC3480.2581832210582545No Hit
CGTGAGCGCTTAACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGC3470.25744131525061575No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG3280.2433451049054812No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG3210.23815176425201057No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC3160.23444223521381724No Hit
CACTTATACCTGTACCTGCTTGATAAAAACATAATGGAAAGGGAAGCCAG3150.2337003294061786No Hit
GGTATATGCAAACCTACCCAGAAAGGCCAAGGAAGTTGGAAAGCTGAAGA3130.23221651779090127No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC3080.22850698875270795No Hit
TTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTT2970.22034602486868268No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC2970.22034602486868268No Hit
CGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAG2970.22034602486868268No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC2950.21886221325340535No Hit
AGATCACGCACTAACATCACAATTAAAAGAACTAAAGAAGCAAGAGCAAA2940.21812030744576666No Hit
TAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTT2860.2121850609846574No Hit
CGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTT2820.20921743775410276No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC2800.20773362613882543No Hit
CTACCAGTAAGTATTTCAGAGCCCAAAACGCGAAAACAAATGGAAGAAGT2780.2062498145235481No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC2760.20476600290827077No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG2740.20328219129299346No Hit
GCTATATACAGGATACTTTCTAGGCAGGGCGCGGTGGCTCACTCCTGTAA2700.2003145680624388No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG2580.19141169837077485No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA2550.18918598094785885No Hit
GGTATATGCAGAGGGATGGGCAGAGCTTAGAGACTGGCTGTAGATAGTAA2530.18770216933258155No Hit
CTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGAT2470.18325073448674956No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACTAAATATTATATTAGTTTTTCT2460.1825088286791109No Hit
CTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAAT2420.17954120544855626No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT2420.17954120544855626No Hit
CTTTTAAAGAAACCAGCTCCTGGCTTCATTGAATTTTGAAGGTTTTTTTG2340.17360595898744693No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA2330.17286405317980827No Hit
GAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTA2280.169154524141615No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG2210.16396118348814434No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG2190.16247737187286704No Hit
GAGCAGAACTGAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATG2180.16173546606522837No Hit
GCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGA2170.1609935602575897No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA2100.15580021960411905No Hit
CTTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGCTCACGAGTGACACCA2100.15580021960411905No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG1990.14763925572009376No Hit
GTTTGGAACTTTAATTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATT1960.1454135382971778No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT1940.14392972668190046No Hit
GGTCAGGAGTTTGAGACCAGCCGGGCCAACATGGTGAAACCCTCTCTCTA1930.1431878208742618No Hit
ATTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAA1910.14170400925898446No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG1890.14022019764370713No Hit
ACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTA1870.13873638602842983No Hit
CTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGTCTATCAATT1860.13799448022079117No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCAC1860.13799448022079117No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG1860.13799448022079117No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG1850.13725257441315253No Hit
GTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGACAGCTAGCGGT1850.13725257441315253No Hit
GGTATATGCTCAGCTTCTTCTGAGGCATCAGGAAGTTTACAATCATGGCA1840.13651066860551386No Hit
GTCTATCAATTTTGTTGATCTTTTAAAGAAACCAGCTCCTGGCTTCATTG1830.1357687627978752No Hit
GTGCAGGTATATGCAAACCTACCCAGAAAGGCCAAGGAAGTTGGAAAGCT1830.1357687627978752No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT1820.13502685699023653No Hit
AACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGAGTAT1800.1335430453749592No Hit
TTATATGCCTTACTCCCAGATGACAGGAGTTTACAATCCGAAGACCGTCA1780.13205923375968187No Hit
GTTTTTCTGTTTGGAACTTTAATTTTAGAGTATCATGACGTTCGTGTTGT1770.1313173279520432No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC1750.12983351633676588No Hit
ATTAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAA1740.12909161052912724No Hit
GGTATATGCTTTGGGAAAACAAGTCCTGTTTTGAGATTTCCAGCTGTCCT1710.12686589310621124No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC1710.12686589310621124No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA1710.12686589310621124No Hit
CTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATT1700.12612398729857258No Hit
ACAAAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCAC1700.12612398729857258No Hit
TTATATGCCTTTTCCCTAAAAGCTTGCTATATACAGGATACTTTCTAGGC1690.1253820814909339No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTTTGATAATGGAC1680.12464017568329525No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC1670.12389826987565658No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG1650.12241445826037926No Hit
TAAAAGAACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGG1610.11944683502982462No Hit
GAACTGAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATGAAGCC1590.11796302341454729No Hit
CTATCAATTTTGTTGATCTTTTAAAGAAACCAGCTCCTGGCTTCATTGAA1580.11722111760690862No Hit
GTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAA1570.11647921179926995No Hit
AACTAAAGAAGCAAGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAA1550.11499540018399264No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG1550.11499540018399264No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA1530.11351158856871531No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC1530.11351158856871531No Hit
AGAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAATTAAGATCAGAG1520.11276968276107664No Hit
CATTTATTATTTGTAATAAAAACATTCACTGTTTTCTGTGTGTGTTTATT1510.11202777695343798No Hit
AAGCACTCGTGGATGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCG1510.11202777695343798No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT1500.11128587114579933No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC1500.11128587114579933No Hit
GAAAGAGACAGAGACACAAAAAAACCTTCAAAATTCAATGAAGCCAGGAG1490.11054396533816066No Hit
GAGCAAACAGATTCACAAGCTAGCAGAAGGCAAGAAATTAAGATCAGAGC1460.10831824791524469No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC1450.10757634210760603No Hit
AACATGCACTGTCCGTTAGGTTCCTGTACTCCTAAAATTGGAGGATTGCA1450.10757634210760603No Hit
GAGCAGGTGGTGTCACTCGTGAGCGCTTAACAAAGCACTCGTGGATGGTG1440.10683443629996736No Hit
CGTGGACAGCTAGCGGTCTATCAATTTTGTTGATCTTTTAAAGAAACCAG1430.1060925304923287No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA1420.10535062468469004No Hit
GTTCTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTA1420.10535062468469004No Hit
ATTCACAAGCTAGCAGAAGGCAAGAAATTAAGATCAGAGCAGAACTGAAA1400.10386681306941271No Hit
GGTTATTAGTTCACCCTTATGACCTGTTTTGATTACCAAAGGTGAAAGTC1400.10386681306941271No Hit
TTGTAGATCTGTTCTCTAAACGAACTAAATATTATATTAGTTTTTCTGTT1380.10238300145413538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATG100.0070841033145.05
GATCCGT100.0070841033145.07
GATGCAA100.0070841033145.09
TAGATCC100.0070841033145.05
TTGTCTA404.783942E-9108.752
AACCCTA406.088612E-790.6255
TTAACCC406.088612E-790.6253
GGTATAT2500.089.91
GCCTTAC259.3690056E-487.07
AGTACTA259.3690056E-487.09
TCAAATG502.2537279E-887.02
ATATGCT1400.082.857144
CTACTTA451.2270848E-680.555569
ATGCTAC451.2270848E-680.555566
TATGCTG451.2270848E-680.555565
TAACCCT451.2270848E-680.555564
ATACTAC554.3655746E-879.090912
TACTACT554.3655746E-879.090913
AGGTATA1200.078.5416645
CAGGTAT1200.078.5416644
Produced by FastQC (version 0.11.5)