FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-65-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-65-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210659
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTCAT20730.9840547994626386No Hit
GTATTACCCTGATGCCAAAATCAGAAAAAAACACATCAAAACAAAAAACA19010.9024062584556083No Hit
GTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTTTTTT11630.5520770534370713No Hit
TTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTG11060.5250191067079973No Hit
CTACCTAGGAACTGGGCCACAGAGTGAGACTCTGTCTCAAAAAAAAAAAA10800.5126768853929811No Hit
ATCATACACTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTT10270.48751774194314035No Hit
TCCATAGACAATGACAGAAGGGAGACGGACAAAGAAAGAGGCTGAGGGAG10020.4756502214479324No Hit
CCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCA9420.4471681722594335No Hit
ACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAAATTGATT9130.43340184848499236No Hit
GCTTAACCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCATT8410.39922338945879365No Hit
GTGCTGGGTGGTTTCCATTTTATTCCTCATCCTGAAGATAGCCCTGAAAG8160.3873558689635857No Hit
GTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTCATCCTGAAGATA7380.35032920501853704No Hit
CTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGAGGA6570.3118784386140635No Hit
GACATGGAATGATCTCTCAGATGTGGCAAATGAAAAAAAGCAATGTATGT6470.3071314304159803No Hit
GTATAAAAATACGTCAGCAGATTATACAACCAACAAAAATCACGAAAAAA6040.28671929516422273No Hit
TTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTACCAA6000.2848204918849895No Hit
GCTCTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGA5800.27532647548882316No Hit
TGACTACACTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATT5800.27532647548882316No Hit
ATCCCCTACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAA5790.2748517746690149No Hit
TGCTTAACCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCAT5040.23924921318339118No Hit
GTTAAATAGCTTGCCTGGTATCACAAAGCTAACATGTGATTGGCTCAGAT5020.23829981154377453No Hit
CTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATCATACA5000.2373504099041579No Hit
ATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTA4990.2368757090843496No Hit
TCCGTGGACCGTGAAGTTGGAAAACCCTCAATTGAATCGTTCCTTCCAGC4960.23545160662492465No Hit
CTATAGGGGGTCGTGGATTGTGTCCTGGACACAATCCCCATTGGGGCTCT4960.23545160662492465No Hit
AGAAATAAGAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTA4950.23497690580511632No Hit
TGTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAA4930.2340275041654997No Hit
GTACTAATTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTG4770.22643229104856666No Hit
GATTTACTTGTGTATGCTGAACCATCCTTGGATCTCTCAGACACATCCCC4700.22310938530990843No Hit
CTTACAAACTCATTCTACAAGGCCAGTATTACCCTGATGCCAAAATCAGA4670.22168528285048347No Hit
ACCCCACACTGCCCTTCCAGCAAAGGTAGCATCCATGTGGAGGCAAGAAT4520.21456477055335874No Hit
CTATAAACACATCTACGCAAATAAACTAGAAAATCTAGACGAAATGGATA4480.2126659672741255No Hit
CACTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTT4420.2098177623552756No Hit
CTTTAATTTACTGGAAATTGATTTTTTAAAAAATGACTACACTGTAACAA4340.20602015579680907No Hit
AAATAAGAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAA4340.20602015579680907No Hit
GCAACAGCCTGCTCTACAAGATGCACCTGTAGTCCCAGCTACTCAGGAGG4180.19842494267987598No Hit
TATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTCCACATGGATG4050.1922538320223679No Hit
GTATATGCCAGCGTGAATGGTTGAGACATGTTAAGGAATCAATTAAGTTA4050.1922538320223679No Hit
CTACTCTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATC4030.19130443038275127No Hit
ACATAACTCCCTTCCCTTTCTGTGTAACAAAGGTCTCTCTGTTATCTCTT3980.1889309262837097No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA3960.18798152464409307No Hit
ACCTAGGAACTGGGCCACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAAA3900.18513331972524316No Hit
GATTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTACC3860.18323451644600988No Hit
ATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGGTCACAAGACACT3850.18275981562620158No Hit
TTTTTAAGTAGTTCAAATTCTGAAACTGTGATTTAAAAATTTTTTACAGT3830.18181041398658496No Hit
GTGTGGGGTAGTGGGAAGGACATAGCATCGGGGTCAGACAGAGGCAGGTT3800.18038631152716No Hit
GTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAAG3800.18038631152716No Hit
GATCTAGGGGGTCACAAGACACTCCCAAATCTAGGGGATCATACACTATC3790.17991161070735168No Hit
CATGAGGGCAGGCCATGGGGGAGATAAGAACCACCAACCTTGGAGAACTT3700.17563930332907685No Hit
ATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAAAACCCT3680.17468990168946022No Hit
TTCTGAAACTGTGATTTAAAAATTTTTTACAGTCAAGCATTCTGATTTTG3550.1685187910319521No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC3410.16187297955463567No Hit
ATTTTGTGCTGACATGGAATGATCTCTCAGATGTGGCAAATGAAAAAAAG3390.16092357791501907No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3370.15997417627540242No Hit
CTCCCAAATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTC3360.15949947545559412No Hit
CTACACTTGAGTATAAAAATACGTCAGCAGATTATACAACCAACAAAAAT3340.15855007381597747No Hit
AATTATATGCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGG3310.1571259713565525No Hit
CCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGAGGAATTGCTTGAACC3260.15475246725751096No Hit
AAACTACACTTGAGTATAAAAATACGTCAGCAGATTATACAACCAACAAA3240.1538030656178943No Hit
AATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG3160.1500054590594278No Hit
TTTTTAATGTGTTGTTGAATTTTGTTTGCTAGTATTTTGTTGAGGATTTC3130.14858135660000285No Hit
CCCTAATTATGAAAAATAGAGGAAGGAATACTTACAAACTCATTCTACAA3110.14763195496038622No Hit
CTACAAGGCCAGTATTACCCTGATGCCAAAATCAGAAAAAAACACATCAA3110.14763195496038622No Hit
ATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTCCGTGGAC3090.14668255332076957No Hit
CCCAAATCTAGGGGATCATACACTATCCACGCAAGCCAGATGGTTGTCCG3080.14620785250096127No Hit
GCCTTAATGCTACTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCC3060.14525845086134465No Hit
GACATGGGACTGGGGAAACAAGACGAGGGAGAGAGGAAAGGAATGAATGC3040.14430904922172802No Hit
GTTATTAATTGATTCATTGGCTTAAAAGGAATAAGATACATTTGCTTTTT3030.1438343484019197No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA3020.14335964758211137No Hit
GGGTAGAGGTTTGCCCCACCGTACCTCAGAACAGTACCCTAGAGCTGCAG2990.1419355451226864No Hit
ATGTTAAATAGCTTGCCTGGTATCACAAAGCTAACATGTGATTGGCTCAG2980.1414608443028781No Hit
GAGCAGGTGGTGTCACTGAATCCCCTACTATGCACTTATCAAGATTTTAC2970.14098614348306981No Hit
ATGCTGAACCATCCTTGGATCTCTCAGACACATCCCCCTTGGTCATGATT2950.14003674184345316No Hit
CATAACTCCCTTCCCTTTCTGTGTAACAAAGGTCTCTCTGTTATCTCTTA2930.13908734020383653No Hit
GCTATGTCCTTCCCACTACCCCACACTGCCCTTCCAGCAAAGGTAGCATC2910.1381379385642199No Hit
CTGTTTATGTGTGTCTGGCACTGTGCTGGGTGGTTTCCATTTTATTCCTC2910.1381379385642199No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2900.13766323774441158No Hit
CCTGAAGATAGCCCTGAAAGATATTGTTTATCTTGATTTTTCAGATGAGG2860.1357644344651783No Hit
GACAAGGGACTGGGGAAACAAGACGAGGGAGAGAGGAAAGGAATGAATGC2850.13528973364537No Hit
CTCCCCAACATATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTC2830.13434033200575338No Hit
AAATAGAGGAAGGAATACTTACAAACTCATTCTACAAGGCCAGTATTACC2830.13434033200575338No Hit
AGATTATACAACCAACAAAAATCACGAAAAAAAAATTAAGATTAAATTAC2750.13054272544728684No Hit
CTGTAACAAGGGAAGGGATCTGGGTTTTTTTGTTGTTTTATTCTTGTTTT2730.12959332380767022No Hit
GTTTTATTCTTGTTTTTTTTAAGTAGTTCAAATTCTGAAACTGTGATTTA2710.1286439221680536No Hit
TCTTTAATGTGTAGTGTTTGTTCTTTATGAGCTCAAAGTGGCTGCTGTAG2690.12769452052843697No Hit
CCCCAACATATCTGCACTTCCTGCAAATTGGAGCCCAATTCTTGCCTCCA2690.12769452052843697No Hit
GACAATGACAGAAGGGAGACGGACAAAGAAAGAGGCTGAGGGAGATGTAA2660.126270418069012No Hit
GGCATAGAGAGGCTTAAAAACTTGTCTAAGATTCTCTAGCCTCTGCCCAA2660.126270418069012No Hit
ATATTGTTTATCTTGATTTTTCAGATGAGGAATCAGGGCTCAGAGATGTT2650.1257957172492037No Hit
GGGTAATAATGACTTGTTGGTGATTGTAGATATTGGGCTGTTAATTGTCA2640.12532101642939536No Hit
CTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAAAACC2630.12484631560958706No Hit
GAGTTGACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAACATCCA2610.12389691396997042No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2600.12342221315016211No Hit
CCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGGTCACA2580.12247281151054547No Hit
AACTAATACTGATACAGAAATCCTCAACAAAATACTAGCAAACAAAATTC2570.12199811069073715No Hit
GACCTAATCTTCAAACTTTTAGGAGAACAGAGAGGTAAACATCCATAGAC2570.12199811069073715No Hit
CTATAGAAGGCAAAGGTTAAAGAGAAGGGTGTCAGGAGAGATGTTGAGTG2560.12152340987092886No Hit
TACACTATCCACGCAAGCCAGATGGTTGTCCGTGGACCGTGAAGTTGGAA2560.12152340987092886No Hit
GTATGTTATTAATTGATTCATTGGCTTAAAAGGAATAAGATACATTTGCT2550.12104870905112054No Hit
GTATTAGTTGGGGATACTGAACTGTAGTTTTTTTTGGTTTGTTTTTTGTT2540.12057400823131222No Hit
CACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCTAGGGGG2540.12057400823131222No Hit
CTGATATCCTGAGTCTCTCCCCAACATATCTGCACTTCCTGCAAATTGGA2500.11867520495207895No Hit
GGACATAGCATCGGGGTCAGACAGAGGCAGGTTTAGAAATCTGAGCCAAT2490.11820050413227064No Hit
CTCAGACACATCCCCCTTGGTCATGATTAATAACTTTTTTAATGTGTTGT2480.11772580331246232No Hit
CAATTGAATCGTTCCTTCCAGCTCTGATATCCTGAGTCTCTCCCCAACAT2480.11772580331246232No Hit
CTACTATGCACTTATCAAGATTTTACTTACTTTAATTTACTGGAAATTGA2460.11677640167284568No Hit
GAAAGATATTGTTTATCTTGATTTTTCAGATGAGGAATCAGGGCTCAGAG2460.11677640167284568No Hit
GCTAACATGTGATTGGCTCAGATTTCTAAACCTGCCTCTGTCTGACCCCG2450.11630170085303738No Hit
CACTAATGCCACAGAAATACAAACTACCATCAGCGAATACTATAAACACA2440.11582700003322904No Hit
GATCACGCACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATC2440.11582700003322904No Hit
GTGTCACTGAATCCCCTACTATGCACTTATCAAGATTTTACTTACTTTAA2430.11535229921342074No Hit
GTCAAGCATTCTGATTTTGAACATAACTCCCTTCCCTTTCTGTGTAACAA2420.11487759839361243No Hit
ATTTTGTGCTGACATGAGGGCAGGCCATGGGGGAGATAAGAACCACCAAC2420.11487759839361243No Hit
ACTATAAACACATCTACGCAAATAAACTAGAAAATCTAGACGAAATGGAT2400.11392819675399579No Hit
TCCTTGGATCTCTCAGACACATCCCCCTTGGTCATGATTAATAACTTTTT2380.11297879511437917No Hit
TATCAAGATTTTACTTACTTTAATTTACTGGAAATTGATTTTTTAAAAAA2370.11250409429457084No Hit
TTGTTACACAGAAAGGGAAGGGAGTTATGTTCAAAATCAGAATGCTTGAC2350.11155469265495421No Hit
TTTCTAAACCTGCCTCTGTCTGACCCCGATGCTATGTCCTTCCCACTACC2320.11013059019552927No Hit
GTCTGACCCCGATGCTATGTCCTTCCCACTACCCCACACTGCCCTTCCAG2310.10965588937572096No Hit
ATGTATGTAGTGAGTATGTTATTAATTGATTCATTGGCTTAAAAGGAATA2310.10965588937572096No Hit
ATGAAAAATAGAGGAAGGAATACTTACAAACTCATTCTACAAGGCCAGTA2290.10870648773610432No Hit
ATTTTATTCCTCATCCTGAAGATAGCCCTGAAAGATATTGTTTATCTTGA2290.10870648773610432No Hit
AGATAACAGAGAGACCTTTGTTACACAGAAAGGGAAGGGAGTTATGTTCA2230.10585828281725441No Hit
GAAAGAGGCTGAGGGAGATGTAACAAAATTTAAGAGATAACAGAGAGACC2220.1053835819974461No Hit
GTACATATACCATGTTTTCCTTATCTATTCACCTGTCGATGGACACTTAG2210.1049088811776378No Hit
ACACACTACTCTCCAGATCTAGGGGGTCACAAGACACTCCCAAATCTAGG2190.10395947953802116No Hit
GAACTTAACATTGTAAATTAGGAATAAGTCTGAACAAGTAAGCACCTAAA2180.10348477871821285No Hit
CTGTTATCTCTTAAATTTTGTTACATCTCCCTCAGCCTCTTTCTTTGTCC2160.10253537707859621No Hit
ATCACGCACTACCCCTGAATCCAGGGGGACACACACTACTCTCCAGATCT2150.1020606762587879No Hit
GTGTAACAAAGGTCTCTCTGTTATCTCTTAAATTTTGTTACATCTCCCTC2150.1020606762587879No Hit
GTCCTTCCCACTACCCCACACTGCCCTTCCAGCAAAGGTAGCATCCATGT2130.10111127461917126No Hit
GGATCTGGGTTTTTTTGTTGTTTTATTCTTGTTTTTTTTAAGTAGTTCAA2120.10063657379936294No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAT100.0070869173145.04
CGGCAAT151.2286383E-4145.08
GGATCCC100.0070869173145.06
TTATCGG151.2286383E-4145.04
TCGGCAA151.2286383E-4145.07
TATCGGC151.2286383E-4145.05
AATGTAG100.0070869173145.04
GGCAATT203.861775E-4108.759
ACTTTAC203.861775E-4108.751
CGGCCGG301.5965157E-596.666664145
GTTCACA301.5965157E-596.6666649
GCTGACC554.1472958E-1092.2727368
TACCTAA406.1006176E-790.6252
GAACTTA502.2599124E-887.01
ACTCGTA259.376391E-487.02
TATGCAT353.430969E-582.857145
TTTGTGC1950.081.794863
TTGTGCT2150.074.186044
GACATGG2250.074.111111
CCAGCGT1100.072.500018
Produced by FastQC (version 0.11.5)