FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-64-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-64-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189990
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG178749.407863571766935No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC150057.897784093899679No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT148237.801989578398863No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG144977.630401600084215No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG82844.360229485762408No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA74493.920732670140534No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA69483.657034580767409No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTG67443.5496605084478134No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA60393.178588346755092No Hit
GATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCA45102.3738091478498866No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG42542.2390652139586296No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA35801.8843097005105531No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA31151.6395599768408864No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA30261.592715406074004No Hit
TCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA27831.464813937575662No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATA25831.359545239223117No Hit
TTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC25671.3511237433549135No Hit
TCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCA23841.254802884362335No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT22991.2100636875625033No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCT21571.1353229117321963No Hit
GAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGAT20261.0663719143112795No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC18380.9674193378598874No Hit
TGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAA16940.8916258750460551No Hit
CTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC16840.8863624401284279No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGT16830.8858360966366651No Hit
CACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATA15240.8021474814463919No Hit
GTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAG14600.7684614979735775No Hit
CATGAAGACAGTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC13750.723722301173746No Hit
CAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCG13380.7042475919785252No Hit
GCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC12970.6826675088162535No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGA11340.5968735196589294No Hit
AACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT11310.5952944891836413No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCAT10280.5410811095320807No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGT9900.5210800568450971No Hit
GTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGG9630.5068687825675036No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCT9540.502131691141639No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATT8460.44528659403126486No Hit
GCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG7720.40633717564082317No Hit
CGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA7650.4026527711984841No Hit
CCATGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC6420.337912521711669No Hit
TGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCG5920.3115953471235328No Hit
GTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC5830.3068582556976683No Hit
ACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAA5530.29106795094478655No Hit
CCAATATTCCTGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT5210.2742249592083794No Hit
CAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGG5050.2658034633401758No Hit
GGTTCATGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG4990.26264540238959944No Hit
ACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTT4220.2221169535238697No Hit
CGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTG4130.21737986209800514No Hit
CTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGC3950.20790567924627612No Hit
ATTCCAGGCATCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG3070.16158745197115637No Hit
CACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGC3020.15895573451234277No Hit
ACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGC2800.14737617769356282No Hit
CCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAAT2670.1405337123006474No Hit
GGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGT2670.1405337123006474No Hit
TATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG2640.13895468182535922No Hit
AGACAGTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTG2490.13105952944891835No Hit
CCTGTTTGCGTATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTG2390.12579609453129112No Hit
ATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAA2260.1189536291383757No Hit
GCCTGGAATACTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA2150.11316385072898574No Hit
CGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCT2110.11105847676193485No Hit
GTTCACCAATTTTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA2030.10684772882783304No Hit
CACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGA1920.10105795041844307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAT100.007086373145.07
CTGAATG100.007086373145.04
ATAGATA100.007086373145.04
CCTAGCA100.007086373145.02
TATAGAT100.007086373145.03
CCCTAGC100.007086373145.01
TAGATAA100.007086373145.05
CATGAAG1500.0135.333331
ATGAAGA1550.0130.967742
TGAAGAC1800.0112.777783
GAAGACA1950.0107.820524
GACAGTG2050.0102.5609747
AGACAGT2050.0102.5609746
AAGACAG2100.0100.119055
ACAGTGT2150.097.79078
CAGTGTT2150.097.79079
TACTGGT554.3752152E-879.090919
ATACTGG554.3752152E-879.090918
GAATACT607.996641E-872.56
GCGTAAA300.001933320872.5145
Produced by FastQC (version 0.11.5)