FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-63-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-63-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243303
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA18970.7796862348594139No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA15290.6284345034792008No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT14540.59760874300769No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA14470.5947316720303489No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG10760.4422469102312754No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA10740.44142488995203516No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC10530.4327936770200121No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA9650.3966247847334394No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT9380.3855275109636955No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG9290.3818284197071142No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC9100.37401922705433144No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT8220.3378503347677587No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT8130.3341512435111774No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC8080.3320961928130767No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT7790.3201768987640925No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA7650.3144227568094105No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA7590.3119566959716896No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC7230.2971603309453644No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA7150.29387224982840326No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT7110.29222820926992266No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC6990.28729608759448094No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT6810.27989790508131834No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG6590.2708556820096752No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA6480.26633457047385356No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA6290.2585253778210709No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT6270.25770335754183055No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT6190.2544152764248694No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC6120.25153820544752836No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT6010.24701709391170681No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA5970.2453730533532262No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT5880.2416739620966449No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT5820.23920790125892405No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC5570.22893264776842046No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG5540.22769961734956No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG5450.2240005260929787No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA5220.21454729288171542No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT5190.21331426246285495No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA5140.21125921176475423No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC5030.20673810022893263No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG4650.19111971492336716No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA4420.18166648171210384No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA4370.17961143101400312No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA4330.17796739045552254No Hit
GTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTAC4320.17755638031590237No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG4310.17714537017628224No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG4310.17714537017628224No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG4250.17467930933856138No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT4170.17139122822160022No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC4100.1685141572442592No Hit
TAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCC4000.16440405584805778No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT3970.16317102542919734No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC3960.16276001528957718No Hit
CATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGT3950.16234900514995704No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC3940.1619379950103369No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC3920.16111597473109662No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC3900.16029395445185635No Hit
CTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTAT3870.15906092403299588No Hit
CACTAGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTG3860.15864991389337574No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3810.15659486319527502No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA3800.15618385305565488No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC3770.15495082263679447No Hit
TTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGT3740.153717792217934No Hit
CTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTC3690.1516627415198333No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT3660.15042971110097286No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3660.15042971110097286No Hit
CAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACA3620.14878567054249228No Hit
ATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTA3620.14878567054249228No Hit
ATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAA3610.14837466040287214No Hit
ATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAA3590.14755264012363184No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT3550.1459085995651513No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA3550.1459085995651513No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT3540.14549758942553115No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT3530.14508657928591098No Hit
TGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTG3520.14467556914629082No Hit
GTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTG3480.14303152858781026No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC3460.14220950830857No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3440.14138748802932968No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT3430.14097647788970954No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG3420.1405654677500894No Hit
CAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAG3270.13440031565578722No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT3260.13398930551616708No Hit
GATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACATAACAGCAGT3250.13357829537654695No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC3250.13357829537654695No Hit
GTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATA3230.13275627509730664No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA3210.13193425481806637No Hit
TTACAAAATTGAAATTCACAGACTTTAATAACAACATTAGTAGCGTTATT3200.13152324467844623No Hit
GAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTT3180.13070122439920595No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT3170.13029021425958578No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA3130.1286461737011052No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT3110.12782415342186493No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA3100.1274131432822448No Hit
ACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAA3080.12659112300300449No Hit
ATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTT3010.12371405202566348No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC3000.12330304188604332No Hit
GTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATT2990.12289203174642319No Hit
ACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCA2970.1220700114671829No Hit
AAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGA2970.1220700114671829No Hit
GTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAG2960.12165900132756276No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT2960.12165900132756276No Hit
TTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTT2950.12124799118794262No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT2930.12042597090870232No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC2930.12042597090870232No Hit
GTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCC2920.12001496076908216No Hit
AGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATG2900.11919294048984189No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT2870.11795991007098146No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA2860.1175488999313613No Hit
GATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACA2860.1175488999313613No Hit
TTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTC2830.11631586951250086No Hit
CACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCA2830.11631586951250086No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT2810.11549384923326057No Hit
GTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCTT2790.1146718289540203No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG2780.11426081881440014No Hit
ATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCC2780.11426081881440014No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA2770.11384980867478No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC2760.11343879853515987No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2750.11302778839553972No Hit
GTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTT2690.11056172755781885No Hit
ACTAAGAAGAGATAAAATCATATCATTGATTTGACCTTCTTTTAAAGACA2690.11056172755781885No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC2670.10973970727857855No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2670.10973970727857855No Hit
CCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATT2660.10932869713895842No Hit
CCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTT2640.10850667685971813No Hit
GTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTG2640.10850667685971813No Hit
AGCTTATAAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCAC2630.10809566672009799No Hit
TTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAAT2630.10809566672009799No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG2630.10809566672009799No Hit
GATAATTACATCCAATTAAAAATGCTTCAGATGATGACGCATTCACATTA2620.10768465658047785No Hit
TAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAAC2600.10686263630123756No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA2590.1064516261616174No Hit
CCATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTG2580.10604061602199726No Hit
GATTTGACCTTCTTTTAAAGACATAACAGCAGTACCCCTTAATTTAAGGG2550.10480758560313683No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT2540.10439657546351669No Hit
GTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGT2540.10439657546351669No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT2520.1035745551842764No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT2510.10316354504465626No Hit
CTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTT2500.10275253490503611No Hit
GACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTT2490.10234152476541597No Hit
AATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCA2480.1019305146257958No Hit
ACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAA2480.1019305146257958No Hit
GGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACATGTATAGCATG2460.10110849434655553No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA2450.10069748420693538No Hit
TCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAGGACAGGGTTA2450.10069748420693538No Hit
GACATATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCAC2450.10069748420693538No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT2440.10028647406731524No Hit
ACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTC2440.10028647406731524No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATC100.007087588145.01
CAACTTA100.007087588145.06
TAAGATA202.1273336E-6145.06
AGGTTGG100.007087588145.08
GATCACG100.007087588145.01
ATCACGC100.007087588145.02
AGTTCCA100.007087588145.08
GTAGTTC100.007087588145.06
AGATAAG202.1273336E-6145.08
CAGCTTA100.007087588145.06
GTTCCTA351.897206E-9124.285711
AACCCTA203.862503E-4108.755
CTTAACC203.862503E-4108.752
CCCTAAT203.862503E-4108.757
ATAAGAT301.5970156E-596.6666645
TCCTAGG451.0881195E-896.6666643
CCTAATT259.3781506E-487.08
TTAACCC259.3781506E-487.03
CCTAGGT502.2613676E-887.04
CCGTTCA608.258212E-1084.583336145
Produced by FastQC (version 0.11.5)