FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-62-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-62-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44527
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAG12802.8746603184584636No Hit
CCCTAGCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGT8481.9044624609787322No Hit
CTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCT6551.4710175848361668No Hit
GTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAA4270.9589687156107531No Hit
GGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTC3910.8781188941541087No Hit
CATGAAGACAGTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTG3600.8084982145664428No Hit
GACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAA3430.7703191322119164No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT3100.6962067958766591No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG3090.6939609675028634No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3030.6804859972600894No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC2970.6670110270173154No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA2900.6512902284007457No Hit
GTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCC2500.5614570934489187No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC2230.5008197273564354No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC2220.4985738989826397No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2210.4963280706088441No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT2200.4940822422350484No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA2120.47611561524468304No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT2070.46488647337570466No Hit
CTCCCTAGCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTG1960.44018236126395227No Hit
CAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCA1940.4356907045163608No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1940.4356907045163608No Hit
GTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAA1940.4356907045163608No Hit
AGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATT1910.4289532193949739No Hit
CTTATGAAGCTTGGTTTGGCTGGATATGAAATTCTGGGTTGAAAATTATT1890.42446156264738255No Hit
GTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAAT1880.42221573427358683No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT1880.42221573427358683No Hit
TCCTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGG1800.4042491072832214No Hit
GACTAACAGCTGATCTCTCAGCAGGAACTCTACAAGCCAGAAGAGAGTGG1780.39975745053563005No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1770.39751162216183444No Hit
ATATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCC1750.3930199654142431No Hit
CCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAA1740.3907741370404474No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT1620.3638241965548993No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC1620.3638241965548993No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1610.3615783681811036No Hit
CATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGG1550.34810339793832956No Hit
TTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTG1540.3458575695645339No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT1520.34136591281694256No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC1510.33912008444314684No Hit
GCATTGATGATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGG1500.3368742560693512No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1450.32564511420037284No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1410.3166618007051901No Hit
CTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACAAAATCTCTCGGCA1390.3121701439575988No Hit
CTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTC1370.3076784872100074No Hit
GTTGAATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTTCCTGCTGAGA1340.3009410020886204No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1340.3009410020886204No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1320.29644934534102907No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG1290.289711860219642No Hit
TCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTT1280.28746603184584635No Hit
CTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAA1270.2852202034720507No Hit
GGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAGTATTT1190.2672535764816853No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC1160.26051609136029824No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG1160.26051609136029824No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA1140.2560244346127069No Hit
GTAATAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAA1140.2560244346127069No Hit
CCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATTTT1100.2470411211175242No Hit
CTTCTGGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATGGG1090.24479529274372852No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1070.2403036359961372No Hit
GTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATT1020.2290744941271588No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC1020.2290744941271588No Hit
GTGTGAGAGAGACAGGCAGAGAGAGACAGAGAGAAGGAAAGAGAGAGACA1010.22682866575336313No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1000.22458283737956747No Hit
GAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAAAATAATTTTCAA970.2178453522581804No Hit
CCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAACT960.21559952388438475No Hit
GGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAACTCTACAAG950.21335369551058908No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG950.21335369551058908No Hit
GCATTTGCTTGTCTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTT940.21110786713679341No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG940.21110786713679341No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG920.20661621038920208No Hit
GTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTA900.2021245536416107No Hit
TTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCT880.19763289689401936No Hit
TTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCTTTCCA880.19763289689401936No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG860.19314124014642803No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT850.19089541177263233No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA810.18191209827744964No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT800.17966626990365397No Hit
AAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTC770.17292878478226695No Hit
GGCCATTCCTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTG750.1684371280346756No Hit
ATAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACAT730.16394547128708425No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT730.16394547128708425No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA720.16169964291328856No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC720.16169964291328856No Hit
GATCTTTACAATTTGGCATGTTTTTGCAGTGGCTGGTACCGGTTGTTCCT720.16169964291328856No Hit
ATGTTAAGGGCAGCCAGAGAGAAAAGTCAGGTTACCCACAAGGGGAAGCC710.1594538145394929No Hit
ACATGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCA710.1594538145394929No Hit
CTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAATTCTGGGTTGA710.1594538145394929No Hit
CAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC710.1594538145394929No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA700.15720798616569723No Hit
CTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAAGAA690.15496215779190156No Hit
CACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAA680.15271632941810587No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC680.15271632941810587No Hit
GTTAAGGGCAGCCAGAGAGAAAAGTCAGGTTACCCACAAGGGGAAGCCCA670.1504705010443102No Hit
GGTTACCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAG670.1504705010443102No Hit
GAACTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCAACATTCTTAAA670.1504705010443102No Hit
CGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGC670.1504705010443102No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG660.14822467267051453No Hit
GGGCAGGCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAG660.14822467267051453No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC650.14597884429671884No Hit
TGAAGACAGTGTGGCCATTCCTCAAAGACCTAGACACATAATTGTCAGAT650.14597884429671884No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC650.14597884429671884No Hit
CCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAAT640.14373301592292317No Hit
ACCCACAAGGGGAAGCCCATCAGACTAACAGCTGATCTCTCAGCAGGAAC630.1414871875491275No Hit
GGCCAATATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCA620.1392413591753318No Hit
ATTCAACATTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCCAG620.1392413591753318No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC620.1392413591753318No Hit
GGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATGGGCTTCC610.13699553080153615No Hit
GGTACCGGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTT600.13474970242774048No Hit
GGTTGTTCCTTTCCATTTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGCA600.13474970242774048No Hit
GCCTGGTGGTGACAAAATCTCTCGGCATTTGCTTGTCTGTAAAGTATTTT600.13474970242774048No Hit
CTCATACACCCTTCACCACTCTGTGGTTGGCTTGCCCTTGGCAGGCATGG600.13474970242774048No Hit
ATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTTCCTGCTGAGAGATCA600.13474970242774048No Hit
TTCTTAAAGAAAATAATTTTCAACCCAGAATTTCATATCCAGCCAAACCA590.13250387405394481No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC590.13250387405394481No Hit
GATACATGGTGGGTGGGTAGATGGGTTGGTGGATGGGAAATTTTAAAGAA590.13250387405394481No Hit
GAGAAAAGTCAGGTTACCCACAAGGGGAAGCCCATCAGACTAACAGCTGA590.13250387405394481No Hit
GGTCTTTCTACCCATAGCACAAGTAGGTAGATGCCTCCATGATGAGATGA580.13025804568014912No Hit
CTTTAAGAATGTTGAATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTT580.13025804568014912No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA580.13025804568014912No Hit
CTCAAAGACCTAGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAG570.12801221730635345No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA560.1257663889325578No Hit
CTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTT560.1257663889325578No Hit
TCTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGG550.1235205605587621No Hit
CATGAAGACAGTGTGAGAGAGACAGGCAGAGAGAGACAGAGAGAAGGAAA540.12127473218496643No Hit
ATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTC540.12127473218496643No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG540.12127473218496643No Hit
GGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTG530.11902890381117076No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC530.11902890381117076No Hit
ATGTTGAATATTGGCCCCCACTCTCTTCTGGCTTGTAGAGTTCCTGCTGA530.11902890381117076No Hit
TGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTT520.11678307543737507No Hit
CTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGA510.1145372470635794No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG510.1145372470635794No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT500.11229141868978373No Hit
CATCAGACTAACAGCTGATCTCTCAGCAGGAACTCTACAAGCCAGAAGAG500.11229141868978373No Hit
AGACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTT500.11229141868978373No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT500.11229141868978373No Hit
ACACATAATTGTCAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAA490.11004559031598807No Hit
CTCTTCTGGCTTGTAGAGTTCCTGCTGAGAGATCAGCTGTTAGTCTGATG490.11004559031598807No Hit
AATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTT490.11004559031598807No Hit
CACTTATGAAGCTTGGTTTGGCTGGATATGAAATTCTGGGTTGAAAATTA490.11004559031598807No Hit
TCTCTCAGCAGGAACTCTACAAGCCAGAAGAGAGTGGGGGCCAATATTCA490.11004559031598807No Hit
GTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAAAGTCAGGT480.10779976194219237No Hit
TTTTTAGTGCTTCCTTCAGGGGCTCTTTTAGGGCAGGCCTGGTGGTGACA480.10779976194219237No Hit
CAGTGTGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTA480.10779976194219237No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG460.10330810519460104No Hit
TACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTG460.10330810519460104No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG460.10330810519460104No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA460.10330810519460104No Hit
ATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGT460.10330810519460104No Hit
ATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGT460.10330810519460104No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA450.10106227682080535No Hit
GTTGTTGTTGTTGTTGTTGTCGTCGTTGCTGTTGTCGTTGTCGTTGTTGT450.10106227682080535No Hit
CTTCATAAGTGAAGGAGAAATAAAATACTTTACAGACAAGCAAATGCCGA450.10106227682080535No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT450.10106227682080535No Hit
GTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTAC450.10106227682080535No Hit
TTGCTTGTCTGTAAAGTATTTTATTTCTCCTTCACTTATGAAGCTTGGTT450.10106227682080535No Hit
ATTTTATTTCTCCTTCACTTATGAAGCTTGGTTTGGCTGGATATGAAATT450.10106227682080535No Hit
GTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCAT450.10106227682080535No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTT151.2221833E-4145.02
TGCGCAT100.0070682745144.999984
CATCTTA100.0070682745144.999986
TGGGGGA100.0070682745144.999984
GGCTCAA100.0070682745144.999981
GGTAGTA202.108076E-6144.999982
GCCATTG100.0070682745144.999981
GCGCATC100.0070682745144.999985
TTGCGCA100.0070682745144.999983
CGCATCT100.0070682745144.999986
GCTCAAT100.0070682745144.999982
TTTGCGC100.0070682745144.999982
TAGGCAG100.0070682745144.999985
CATGAAG2650.0125.849061
ATGAAGA2750.0121.272732
GGGTAGT301.0843178E-7120.8333361
TGAAGAC2800.0119.107143
ACAGTGT3100.0107.580658
CCTGTTG501.891749E-10101.51
GACAGTG3400.0100.220597
Produced by FastQC (version 0.11.5)