FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-61-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-61-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238602
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACT19880.833186645543625No Hit
GGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA16970.711226226100368No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTG13780.5775307834804402No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA11080.4643716314196864No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA10390.4354531814486048No Hit
CACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA10040.42078440247776633No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC9850.412821351036454No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG8990.37677806556525095No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA8830.37007233803572476No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC8670.3633666105061986No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC8460.35456534312369553No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA8380.35121247935893246No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATAT8130.34073478009404784No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA7690.3222940293878509No Hit
CCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATA7580.3176838417113017No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT7570.3172647337407063No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC7570.3172647337407063No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG7490.3139118699759432No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA7470.31307365403475246No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT7460.31265454606415705No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC6960.2916991475343878No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC6820.28583163594605243No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT6740.28247877218128936No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA6530.2736775047987863No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC6520.27325839682819086No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA6510.2728392888575955No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA6350.26613356132806937No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA6280.26319980553390165No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA6150.25775140191616164No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACC6090.25523675409258934No Hit
GTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG6080.2548176461219939No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT5870.2460163787394909No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA5760.24140619106294164No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC5660.23721511135698778No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA5550.23260492368043859No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA5220.21877436065079084No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA5210.21835525268019546No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG5170.21667882079781392No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT5020.21039220123888316No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG4860.203686473709357No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT4660.19530431429744932No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT4480.1877603708267324No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4400.1844075070619693No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG4290.17979731938542007No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA4290.17979731938542007No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA4250.17812088750303853No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG4190.17560623967946623No Hit
GGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAAC4130.17309159185589393No Hit
GTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCT4110.17225337591470316No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA4090.17141515997351237No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT4090.17141515997351237No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT4080.17099605200291698No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA4050.16973872809113083No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCAC3990.16722408026755853No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG3960.16596675635577238No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTG3930.16470943244398623No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA3780.15842281288505544No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA3780.15842281288505544No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA3670.15381262520850622No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG3650.15297440926731545No Hit
ATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATG3600.1508788694143385No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC3560.149202437531957No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3520.14752600564957544No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG3500.14668778970838467No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT3430.14375403391421698No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA3400.14249671000243083No Hit
GTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAAT3400.14249671000243083No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT3400.14249671000243083No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT3390.14207760203183542No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA3390.14207760203183542No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA3340.13998206217885853No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT3330.13956295420826315No Hit
GTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCAC3280.1374674143552862No Hit
AAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTG3220.1349527665317139No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT3200.13411455059052313No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT3200.13411455059052313No Hit
GGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCAT3160.1324381187081416No Hit
CTGTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCT3150.1320190107375462No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC3130.13118079479635544No Hit
GTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTT3120.13076168682576006No Hit
CGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTA3110.13034257885516468No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA3080.1290852549433785No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA3070.1286661469727831No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA3050.12782793103159235No Hit
ATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGC3020.12657060711980622No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT3020.12657060711980622No Hit
GTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTAT3000.12573239117861543No Hit
CAATCACCTTCTTCTTCATCCTCATCTGGAGGGTAGAAAGAACAATACAT2990.12531328320802004No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT2970.12447506726682928No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2970.12447506726682928No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT2950.12363685132563851No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC2950.12363685132563851No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2920.12237952741385234No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2910.12196041944325696No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT2910.12196041944325696No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC2910.12196041944325696No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT2900.12154131147266159No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA2890.1211222035020662No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG2880.12070309553147082No Hit
GGTTTACCTTGGTAATCATCTTCAGTACCATACTCATATTGAGTTGATGG2860.11986487959028005No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT2860.11986487959028005No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT2860.11986487959028005No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT2840.11902666364908927No Hit
GAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAATCCCT2830.11860755567849389No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2820.1181884477078985No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA2820.1181884477078985No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC2810.11776933973730312No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2810.11776933973730312No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2800.11735023176670774No Hit
AACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACTGA2750.1152546919137308No Hit
CTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCT2740.11483558394313542No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2730.11441647597254005No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2720.11399736800194467No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2710.11357826003134928No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2710.11357826003134928No Hit
CATCTGGAGGGTAGAAAGAACAATACATATGTGAAGCCAATTTAAACTCA2700.1131591520607539No Hit
ACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAA2690.1127400440901585No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2690.1127400440901585No Hit
GTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTT2680.11232093611956312No Hit
ATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGAT2670.11190182814896774No Hit
ATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATT2670.11190182814896774No Hit
GCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACA2650.11106361220777697No Hit
CTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTT2620.10980628829599082No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT2620.10980628829599082No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCC2600.10896807235480004No Hit
CTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACA2600.10896807235480004No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2600.10896807235480004No Hit
CTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATT2600.10896807235480004No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT2600.10896807235480004No Hit
GTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGA2580.10812985641360927No Hit
CCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAAC2570.10771074844301388No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT2560.10729164047241851No Hit
GAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAGTTCAAAAGT2550.10687253250182313No Hit
CATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTTCT2540.10645342453122775No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA2540.10645342453122775No Hit
CATTTACTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTA2530.10603431656063234No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2520.10561520859003695No Hit
GGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGC2520.10561520859003695No Hit
GTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGA2500.1047769926488462No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC2470.10351966873706005No Hit
CCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTT2470.10351966873706005No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2460.10310056076646466No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC2450.10268145279586927No Hit
GAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTAC2440.10226234482527388No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC2420.10142412888408311No Hit
CATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTG2420.10142412888408311No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2390.10016680497229698No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGA100.0070875026145.09
AAGAGCG100.0070875026145.06
TCCCTAG100.0070875026145.02
GAGCGTG100.0070875026145.08
GCTAGTC100.0070875026145.06
AAGGGCT100.0070875026145.06
GACGATT100.0070875026145.09
AGAGCGT100.0070875026145.07
GTCTCAA203.8624104E-4108.751
CCCTAGC301.5969517E-596.666681
GTACAAG300.001933929772.500013
ATATTGT300.001933929772.500014
CGTATAC1100.065.909093
AAGACAG554.052141E-665.909095
GTCGTAT1100.065.909091
CAGTGTT554.052141E-665.909099
TCGTATA1100.065.909092
CAGGGCT1150.063.0434729
GACAGTG606.7897745E-660.416677
AGACAGT606.7897745E-660.416676
Produced by FastQC (version 0.11.5)