FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-61-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-61-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176859
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA10050.5682492833273963No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA9950.5625950616027456No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA6270.3545197021355995No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT5790.32737943785727613No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG5690.32172521613262545No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG5370.30363170661374317No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA4620.26122504367886284No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT4610.26065962150639777No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT4580.2589633549890025No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA4480.2533091332643518No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG4470.25274371109188676No Hit
TATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT4410.24935117805709633No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4240.23973900112519014No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC4160.23521562374546953No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC3990.22560344681356337No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG3980.2250380246410983No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC3870.2188183807439825No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT3760.21259873684686673No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA3750.21203331467440165No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC3630.20524824860482077No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC3540.20015944905263516No Hit
GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA3470.19620149384537966No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3380.19111269429319402No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA3310.18715473908593852No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT3290.18602389474100836No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC3220.18206593953375289No Hit
ATGCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTC3190.18036967301635765No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3120.17641171780910217No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT3110.1758462956366371No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT3030.17132291825691653No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT3000.16962665173952132No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT2980.16849580739459116No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC2980.16849580739459116No Hit
GCTCTACAAGATGCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCA2940.1662341187047309No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT2940.1662341187047309No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC2930.16566869653226582No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2910.16453785218733566No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG2790.1577527861177548No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC2780.15718736394528973No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA2780.15718736394528973No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT2760.1560565196003596No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2690.15209856439310412No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA2680.15153314222063904No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA2590.1464443426684534No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA2530.14305180963366299No Hit
GGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACG2520.1424863874611979No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC2470.13965927659887253No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG2450.1385284322539424No Hit
GCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGA2440.13796301008147735No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA2420.1368321657365472No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT2420.1368321657365472No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT2420.1368321657365472No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG2410.13626674356408214No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2400.13570132139161706No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT2390.13513589921915198No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT2350.1328742105292917No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG2310.13061252183943142No Hit
TCATTAAATGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCC2300.13004709966696634No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT2260.12778541097710608No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA2260.12778541097710608No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC2250.12721998880464097No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG2240.1266545666321759No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC2200.12439287794231563No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC2190.12382745576985056No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT2160.12213118925245535No Hit
GTTTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT2120.11986950056259507No Hit
CTACAAGATGCTTATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACC2120.11986950056259507No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG2100.11873865621766491No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT2090.11817323404519985No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA2090.11817323404519985No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA2090.11817323404519985No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG2080.11760781187273477No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG2070.1170423897002697No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA2050.11591154535533957No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT1920.10856105711329364No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA1900.1074302127683635No Hit
TGGTAGGACAGGGTTATCAAACCTCTTAGTACCATTGGTCCCAGAGACAT1890.10686479059589843No Hit
GTATTGATTTCTGTTCACCAATATTCCAGGCACCTTTTTTAGCTTTTCCT1880.10629936842343336No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT1880.10629936842343336No Hit
GTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGA1870.1057339462509683No Hit
CAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCT1860.10516852407850322No Hit
CAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATA1850.10460310190603815No Hit
AGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAAAAATCCAGCCTCT1820.10290683538864293No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT1820.10290683538864293No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG1810.10234141321617786No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT1800.10177599104371278No Hit
ACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGC1790.10121056887124771No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA1780.10064514669878265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGT100.0070859636145.07
TTAAGGC100.0070859636145.05
AGGTCTA202.1257092E-6145.07
TCCGTTC100.0070859636145.09
AGATCCG100.0070859636145.06
TAGATCC100.0070859636145.05
GTTCCTA607.2759576E-1296.6666641
CTTAACC259.373883E-487.02
TTAACCC259.373883E-487.03
ACTTATC353.4294426E-582.857142
AAATTAG2100.075.952385
AACCCTA300.001933099472.55
CTTATCG406.648045E-572.53
TTCCTAG754.82396E-967.6666642
TTATCGG451.1911532E-464.444444
TATCGGC451.1911532E-464.444445
ATCGGCA451.1911532E-464.444446
TCCTAGG808.034476E-963.43753
CCTAGGT808.034476E-963.43754
TATAAAT2600.061.3461532
Produced by FastQC (version 0.11.5)