FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-60-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-60-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189120
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC22691.1997673434856175No Hit
CCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACT14790.7820431472081218No Hit
CCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT10220.5403976311336717No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG9290.49122250423011843No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA8570.4531514382402707No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC5940.31408629441624364No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG5580.2950507614213198No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA5440.2876480541455161No Hit
TCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA5200.27495769881556686No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4910.25962351945854484No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGT4770.2522208121827411No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC4720.24957698815566837No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT4690.2479906937394247No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG4670.24693316412859562No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT4630.24481810490693737No Hit
GTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA4440.23477157360406092No Hit
GTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGT4330.22895516074450084No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA4310.22789763113367176No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT4030.21309221658206431No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT4020.21256345177664976No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG3980.21044839255499154No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA3870.20463197969543145No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA3840.20304568527918782No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC3790.20040186125211507No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA3710.19617174280879865No Hit
GTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCA3510.18559644670050762No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC3480.18401015228426396No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT3460.18295262267343484No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA3450.18242385786802032No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA3450.18242385786802032No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA3380.17872250423011843No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA3380.17872250423011843No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA3370.17819373942470387No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC3330.17607868020304568No Hit
CAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTG3290.17396362098138748No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT3240.17131979695431473No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3210.16973350253807107No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA3190.16867597292724196No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT3100.163917089678511No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA3100.163917089678511No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA3020.15968697123519457No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT3000.15862944162436549No Hit
AGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA2940.15545685279187818No Hit
CAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGT2930.15492808798646363No Hit
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTC2930.15492808798646363No Hit
ATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC2920.15439932318104907No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA2910.15387055837563451No Hit
ATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATT2900.15334179357021996No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA2890.15281302876480543No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA2880.15228426395939085No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2820.14911167512690354No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT2820.14911167512690354No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT2770.1464678510998308No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2760.14593908629441624No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG2760.14593908629441624No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT2730.14435279187817257No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT2730.14435279187817257No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG2690.1422377326565144No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG2690.1422377326565144No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC2660.14065143824027074No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2650.14012267343485618No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT2640.13959390862944163No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA2600.13747884940778343No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2590.13695008460236888No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2580.13642131979695432No Hit
GATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGC2570.13589255499153977No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA2540.13430626057529613No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT2500.1321912013536379No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA2480.1311336717428088No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC2470.13060490693739424No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT2440.1290186125211506No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG2430.12848984771573604No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2410.12743231810490693No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT2390.12637478849407785No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA2370.1253172588832487No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC2350.12425972927241963No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA2320.12267343485617598No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2300.12161590524534688No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA2290.12108714043993231No Hit
CCTGTAATGTAGGCCATTACAACTAGATTGTTAGTAGCCAAATCAGATGT2280.12055837563451778No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT2270.12002961082910321No Hit
GCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC2270.12002961082910321No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA2260.11950084602368866No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2260.11950084602368866No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2250.11897208121827413No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2210.1168570219966159No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC2190.11579949238578681No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGA2190.11579949238578681No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT2180.11527072758037225No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2170.1147419627749577No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAA2160.11421319796954316No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2160.11421319796954316No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA2150.1136844331641286No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2140.11315566835871403No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA2130.1126269035532995No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2130.1126269035532995No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC2100.11104060913705585No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG2090.11051184433164128No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2080.10998307952622673No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2080.10998307952622673No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2070.10945431472081218No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTG2060.10892554991539763No Hit
ACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGT2040.10786802030456853No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2040.10786802030456853No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2030.10733925549915398No Hit
CATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTTCT2010.10628172588832488No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2010.10628172588832488No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG1990.10522419627749577No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT1990.10522419627749577No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG1960.1036379018612521No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1960.1036379018612521No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT1960.1036379018612521No Hit
TAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCT1950.10310913705583757No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA1950.10310913705583757No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC1950.10310913705583757No Hit
GTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTT1950.10310913705583757No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA1940.102580372250423No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT1930.10205160744500845No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG1920.10152284263959391No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT1900.1004653130287648No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAT100.007086348145.05
AGGGCTC100.007086348145.05
AAGGGTA151.2284408E-4145.02
GGGCTCT100.007086348145.06
GACAGTG600.0132.916677
GAAGACA650.0122.692314
AAGACAG650.0122.692315
AGACAGT650.0122.692316
GCTCAAT301.09821485E-7120.8333362
GTGAATC256.4512187E-6116.06
CAGTGTT650.0111.538469
GGACATT203.8611572E-4108.756
GAAGGGT203.8611572E-4108.751
TCAATGT352.7530223E-7103.571434
CTCAATG352.7530223E-7103.571433
TCCCTAG301.5960908E-596.6666642
CCTAGCA651.4551915E-1189.230772
CCCTAGC651.4551915E-1189.230771
GTCTCAA259.374897E-487.01
CTGCCAT259.374897E-487.03
Produced by FastQC (version 0.11.5)