FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-60-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-60-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239662
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA23580.9838856389415093No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA13020.5432650983468384No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT11000.45897972978611545No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG8490.35424890053492003No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA8390.35007635753686445No Hit
CTTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG8090.33755872854269764No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC7380.30793367325650295No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT7180.2995885872603917No Hit
CCCTTTACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA7180.2995885872603917No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG6950.28999173836486386No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA6710.27997763516953045No Hit
GCTTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT6670.2783086179703082No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT6130.2557768857808079No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC5980.24951807128372458No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAA5830.24325925678664118No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG5690.23741769658936338No Hit
CCCTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTC5610.23407966219091886No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC5560.23199339069189107No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC5550.23157613639208552No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT5510.2299071191928633No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT5330.2223965417963632No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA5300.22114477889694653No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG5250.21905850739791874No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT5230.21822399879830762No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA5190.2165549815990854No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC5030.20987891280219642No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT4970.20737538700336308No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT4910.2048718612045297No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC4700.19610952090861294No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC4620.19277148651016848No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC4510.18818168921230732No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA4500.18776443491250178No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA4440.1852609091136684No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG4420.18442640051405731No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA4420.18442640051405731No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT4370.1823401290150295No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT4350.18150562041541837No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT4340.18108836611561283No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG4340.18108836611561283No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT4330.18067111181580725No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA4070.16982250002086272No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG4020.16773622852183492No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA3980.16606721132261268No Hit
CCCTTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATC3980.16606721132261268No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA3920.1635636855237793No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3920.1635636855237793No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT3890.16231192262436264No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG3880.16189466832455707No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT3810.15897388822591818No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA3790.15813937962630703No Hit
GCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTT3630.1514633108294181No Hit
GTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAA3530.1472907678313625No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG3530.1472907678313625No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG3500.14603900493194583No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC3490.14562175063214028No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT3490.14562175063214028No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT3470.14478724203252916No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT3450.14395273343291803No Hit
CTTGTAGATCTGTTCTCTAAACGAACAATGTTTGTTTTTCTTGTTTTATT3400.14186646193389021No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA3390.14144920763408467No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC3350.13978019043486245No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT3320.13852842753544575No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA3290.13727666463602906No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC3270.13644215603641796No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG3180.13268686733816792No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA3170.13226961303836235No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA3150.13143510443875123No Hit
ATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCT3070.12809707004030677No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG3060.1276798157405012No Hit
CTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTC3040.1268453071408901No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT3010.1255935442414734No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC2990.12475903564186229No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2970.12392452704225118No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA2920.12183825554322336No Hit
GATAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTC2910.12142100124341781No Hit
ATCTAAAGTAGTACCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAG2860.11933472974439002No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA2850.11891747544458446No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG2810.11724845824536222No Hit
GAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGC2810.11724845824536222No Hit
CCTTAGAAACTACAGATAAATCTTGGGATTTTATTATTTCAACAAAATCA2800.11683120394555666No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2780.11599669534594553No Hit
GAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGTA2780.11599669534594553No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC2730.11391042384691775No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT2690.1122414066476955No Hit
GCTTATTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGAT2660.11098964374827883No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT2640.1101551351486677No Hit
GTTTTCCTTCAAGGTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTG2610.10890337224925103No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT2570.10723435505002879No Hit
AGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCACTAGAATA2520.105148083551001No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT2510.10473082925119544No Hit
CTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTA2490.10389632065158433No Hit
GTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGA2480.10347906635177877No Hit
GTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAAGTAGTACCAA2480.10347906635177877No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG2440.10181004915255651No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA2440.10181004915255651No Hit
CATCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGT2400.10014103195333428No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT2400.10014103195333428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA100.007087522145.02
GTCAACT100.007087522145.01
AAATATC100.007087522145.05
ACGTATA100.007087522145.04
GACGGCT100.007087522145.09
CGGACGG100.007087522145.07
AACCCCC100.007087522145.07
TCGGACG100.007087522145.06
GTATAGA100.007087522145.01
CGTATAG100.007087522145.05
AATATCG100.007087522145.06
TATAGAA100.007087522145.02
GGACGGC100.007087522145.08
TTCTATA151.2288478E-4144.999982
CCCTTTA1300.0122.6923141
TCTATAC203.8624316E-4108.753
GTTCCTA352.755296E-7103.571431
TTTTATA1150.0100.869572
AACCCTA301.5969668E-596.666665
TCCTAGG406.1032006E-790.6253
Produced by FastQC (version 0.11.5)