FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-59-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-59-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188215
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC6880.3655394097176102No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG6680.35491326408628426No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA6550.34800626942592244No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT5800.30815822330845044No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA5690.30231384321122123No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT5420.2879685466089313No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT5250.2789363228223043No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT5230.2778737082591717No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG5020.2667162553462795No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT4800.25502749515182105No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT4680.24865180777302553No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA4670.24812050049145923No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC4670.24812050049145923No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT4260.22633690194724118No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC4250.22580559466567487No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC4230.22474298010254232No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC4160.22102382913157825No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA4000.21252291262651757No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA3920.2082724543739872No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA3780.20083415243205907No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT3740.1987089233057939No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG3700.19658369417952876No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC3650.19392715777169725No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG3610.1918019286454321No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA3590.1907393140822995No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA3480.18489493398507026No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3460.18383231942193767No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA3450.1833010121403714No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA3440.1827697048588051No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA3420.1817070902956725No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT3420.1817070902956725No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA3410.1811757830141062No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT3410.1811757830141062No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3400.18064447573253992No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA3310.17586271019844327No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA3300.175331402916877No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA3290.17480009563531068No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA3190.16948702281964773No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA3140.16683048641181628No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT3120.1657678718486837No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT3100.1647052572855511No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG3100.1647052572855511No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT3010.15992349175145446No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT2990.15886087718832187No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2990.15886087718832187No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2990.15886087718832187No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG2980.15832956990675556No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2880.15301649709109263No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA2870.15248518980952633No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2860.15195388252796005No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC2860.15195388252796005No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2840.15089126796482746No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA2830.15035996068326118No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT2830.15035996068326118No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT2810.14929734612012857No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC2770.1471721169938634No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA2770.1471721169938634No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA2750.14610950243073081No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2730.14504688786759823No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC2650.14079642961506786No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT2650.14079642961506786No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2620.139202507770369No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT2620.139202507770369No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA2620.139202507770369No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA2600.1381398932072364No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC2570.13654597136253752No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT2560.13601466408097124No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2550.13548335679940493No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG2530.13442074223627234No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT2530.13442074223627234No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA2520.13388943495470607No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG2500.13282682039157345No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2480.13176420582844087No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA2460.1307015912653083No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG2450.130170283983742No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA2440.12963897670217572No Hit
GTTAGTAGCCTTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGT2430.12910766942060942No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2420.1285763621390431No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA2400.12751374757591052No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2400.12751374757591052No Hit
AAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTG2380.12645113301277794No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG2380.12645113301277794No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG2380.12645113301277794No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2350.12485721116807906No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC2340.12432590388651277No Hit
TATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCT2340.12432590388651277No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA2330.12379459660494646No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT2330.12379459660494646No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT2290.1216693674786813No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC2260.12007544563398241No Hit
CTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATT2250.11954413835241612No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG2230.11848152378928353No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC2220.11795021650771724No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2210.11741890922615093No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT2210.11741890922615093No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA2200.11688760194458465No Hit
CACCAGACTCATCAAATAAGTAGTATGTAGCCATACTCCACTCATCTAAA2190.11635629466301836No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC2190.11635629466301836No Hit
CATTTACTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTA2180.11582498738145205No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC2170.11529368009988578No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC2170.11529368009988578No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG2170.11529368009988578No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA2150.11423106553675319No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC2120.11263714369205431No Hit
GTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTAT2100.11157452912892171No Hit
ATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGAT2100.11157452912892171No Hit
CACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATC2100.11157452912892171No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2100.11157452912892171No Hit
CTTTAAGTGGTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATG2090.1110432218473554No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2090.1110432218473554No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC2090.1110432218473554No Hit
CATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTG2090.1110432218473554No Hit
TTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGCCACAACACAGG2070.10998060728422283No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2060.10944930000265653No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA2040.10838668543952394No Hit
TTTTTAATGTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATT2030.10785537815795765No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2030.10785537815795765No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA2020.10732407087639137No Hit
CATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTTCT2020.10732407087639137No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA2020.10732407087639137No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC2010.10679276359482506No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT2010.10679276359482506No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT1990.10573014903169248No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA1980.10519884175012618No Hit
GTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGA1980.10519884175012618No Hit
CACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAAC1970.1046675344685599No Hit
ATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACA1960.1041362271869936No Hit
ACTTTAAGTGGTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCAT1960.1041362271869936No Hit
TAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCT1950.1036049199054273No Hit
GCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACA1950.1036049199054273No Hit
GATATATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATG1950.1036049199054273No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC1920.10201099806072841No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA1920.10201099806072841No Hit
AAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAA1920.10201099806072841No Hit
CTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCT1910.10147969077916212No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG1910.10147969077916212No Hit
ACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAA1900.10094838349759584No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG1900.10094838349759584No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC1900.10094838349759584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATGC151.2284316E-4145.02
GCCTATG151.2284316E-4145.01
AAGGGTA202.1260676E-6145.02
GAGTTCC100.0070863212145.06
AGAGTTC100.0070863212145.05
AGAGAGC100.0070863212145.03
AGGTCTG100.0070863212145.01
CAGGCGG100.0070863212145.07
GTTAATT100.0070863212145.03
GAAGGGT202.1260676E-6145.01
GAAGACA750.0125.6666644
GAATATT750.0116.01
AATATTG800.0108.752
AAGACAG900.0104.722225
GGTAGTA607.2759576E-1296.6666642
TGAAGAC1000.094.253
GGGTAGT702.910383E-1182.857141
GACAGTG1150.081.956537
TGGTGAA1150.075.6521767
CATGAAG1250.075.41
Produced by FastQC (version 0.11.5)